Mixing Characteristics for Scale‐up of an Orbital Shaken Bioreactor

Abstract

Two types of culture bags are typically used. Among the criteria for evaluating the bioreactor performance, mixing time and shear stress are important indicators. Experiments and numerical analyses are conducted to evaluate and predict the performance of an orbital shaken bioreactor by analyzing mixing time and shear stress. Reliability is ensured by performing numerical analysis under the same conditions using FLUENT. The error between the experimental and numerical analysis results is within the error range of the experiments. For two different working fluid volumes the mixing time and shear stress are predicted using validated numerical models.

Mass Transfer in a Liter-Scale Wave Mixed Single-Use Bioreactor: Influence of Viscosity and Antifoaming Agent

“…Despite the already substantial amount of experimental data regarding wave mixed single-use bioreactors, given the wide interest from researchers and broad applicability in modern areas of biopharmacy, bioengineering, and novel medical therapies, characterization of rocking bioreactors is still desired, as it has been discussed in reports about, e.g., stirred single-use and multi-use reactors (10 –1 to 10 0 dm 3 volume, − 10 1 to 10 2 dm 3 volume, − and 10 3 dm 3 volume − ). Similar to other bioreactor types, such as orbitally shaken single-use bioreactors − and shake flasks − or airlift bioreactors, − it is valuable and relevant to further supplement the base of experimental data about wave mixed devices, including those regarding the mass transfer. The data will subsequently enable researchers to derive empirical correlations for predicting values of important parameters (e.g., mixing time, volumetric mass transfer coefficient) as a function of process variables, such as those defining the wave mixing (rocking angle α, rocking frequency ω, headspace gas flow rate Q G ) or the properties of the liquid phase (temperature T , viscosity μ, volume of liquid phase V L ).…”