Mixing of Shiga toxin-producing and enteropathogenic Escherichia coli in a wastewater treatment plant receiving city and slaughterhouse wastewater

Bibbal, Delphine; Um, Maryse Michèle; Diallo, Alpha; Kérourédan, Monique; Dupouy, Véronique; Toutain, Pierre‐Louis; Bousquet‐mélou, Alain; Oswald, Éric; Brugère, Hubert

doi:10.1016/j.ijheh.2017.12.009

Cited by 11 publications

(11 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there were significant differences among all the genes. Most of the isolates possessed aadA (22.86%), strA (19.85%), and strB (19.38%) genes that code for resistance to streptomycin and similar observations have been reported in other studies (Srinivasan et al, 2007; Shahrani et al, 2014; Bibbal et al, 2017). Despite the fact that tet genes are commonly found in E. coli from cattle due to the high usage of tetracycline in dairy and feedlot cattle, the occurrence of tet resistance genes in E. coli O177 isolates was low.…”

Section: Discussionsupporting

confidence: 86%

“…This therefore creates a knowledge gap on the virulence profiles of aEPEC strains in the area. Despite the fact that aEPEC strains were known to be less pathogenic when compared to EHEC counterparts, data from some recent studies have revealed the presence of virulence determinants in aEPEC strains thus making them to start receiving attention as pathogens of severe clinical significance in humans (Beutin et al, 2005; Bibbal et al, 2017) as well as in food-producing animals, especially sheep and wildlife (Otero et al, 2013; Álvarez-Suárez et al, 2016; Martins et al, 2016). In this study, we expand our previous investigations on aEPEC in the area by determining the occurrence of aEPEC O177 strains in cattle and also provide an overview of the putative virulence and antimicrobial resistance profiles of the isolates.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we expand our previous investigations on aEPEC in the area by determining the occurrence of aEPEC O177 strains in cattle and also provide an overview of the putative virulence and antimicrobial resistance profiles of the isolates. This was motivated from the fact that cattle are the primary reservoir of both EHEC ( stx + ) and aEPEC ( stx − ) strains, thus providing opportunities for horizontal transfer of virulence and antibiotic resistant genes between species and/or strains living in the same ecological niches (Bibbal et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The First Isolation and Molecular Characterization of Shiga Toxin-Producing Virulent Multi-Drug Resistant Atypical Enteropathogenic Escherichia coli O177 Serogroup From South African Cattle

Montso

Mlambo

Ateba

2019

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Atypical enteropathogenic E. coli (aEPEC) is a group of diarrhoeagenic Escherichia coli with high diversity of serogroups, which lack the bundle-forming pili (BFP) and genes encoding for shiga toxins. The aim of this study was to isolate, identify and determine virulence and antibiotic resistance profiles of aEPEC O177 strains from cattle feces. A total of 780 samples were collected from beef and dairy cattle and analyzed for the presence of E. coli O177. One thousand two hundred and seventy-two (1272) presumptive isolates were obtained and 915 were confirmed as E. coli species. Three hundred and seventy-six isolates were positively confirmed as E. coli O177 through amplification of rmlB and wzy gene sequences using multiplex PCR. None of these isolates harbored bfpA gene. A larger proportion (12.74%) of the isolates harbored hlyA gene while 11.20, 9.07, 7.25, 2.60, and 0.63% possessed stx2, stx1, eaeA, stx2a, and stx2d, respectively. Most of E. coli O177 isolates carried stx2/hlyA (9.74%). Furthermore, 7.40% of the isolates harbored stx1/stx2 while 7.09% possessed stx1/stx2/hlyA genes. Only one isolate harbored stx1/stx2/hly/eaeA/stx2a/stx2d while 5.11% of the isolates harbored all the four major virulence genes stx1/stx2/hlyA/eaeA, simultaneously. Further analysis revealed that the isolates displayed varied antimicrobial resistance to erythromycin (63.84%), ampicillin (21.54%), tetracycline (13.37%), streptomycin (17.01%), kanamycin (2.42%), chloramphenicol (1.97%), and norfloxacin (1.40%). Moreover, 20.7% of the isolates exhibited different phenotypic multi-drug resistance patterns. All 73 isolates harbored at least one antimicrobial resistance gene. The aadA, streA, streB, erm, and tetA resistance genes were detected separately and/or concurrently. In conclusion, our findings indicate that environmental isolates of aEPEC O177 strains obtained from cattle in South Africa harbored virulence and antimicrobial resistance gene determinants similar to those reported in other shiga-toxin producing E. coli strains and suggest that these determinants may contribute to the virulence of the isolates.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The First Isolation and Molecular Characterization of Shiga Toxin-Producing Virulent Multi-Drug Resistant Atypical Enteropathogenic Escherichia coli O177 Serogroup From South African Cattle

Montso

Mlambo

Ateba

2019

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Antibiotic-resistant [15,[18][19][20][21][22] Escherichia coli N/A [13,23,24] Echinococcus multilocularis N/A [25] Enterococcus faecium Vancomycin-resistant [21] Klebsiella spp. Antibiotic-resistant [15,21,26] Enterobacter cloacae Antibiotic-resistant [21] Citrobacter spp.…”

Section: Escherichia Colimentioning

confidence: 99%

Characterization of Slaughterhouse Wastewater and Development of Treatment Techniques: A Review

Dalhatou

Wilson

et al. 2022

Processes

View full text Add to dashboard Cite

Commercialization in the meat-processing industry has emerged as one of the major agrobusiness challenges due to the large volume of wastewater produced during slaughtering and cleaning of slaughtering facilities. Slaughterhouse wastewater (SWW) contains proteins, fats, high organic contents, microbes, and other emerging pollutants (pharmaceutical and veterinary residues). It is important to first characterize the wastewater so that adequate treatment techniques can be employed so that discharge of this wastewater does not negatively impact the environment. Conventional characterization bulk parameters of slaughterhouse wastewater include pH, color, turbidity, biochemical oxygen demand (BOD), chemical oxygen demand (COD), total organic carbon (TOC), total suspended solids (TSS), total nitrogen (TN), total phosphorus (TP), and coliform counts. Characterization studies conducted have revealed the effects of the pollutants on microbial activity of SWW through identification of toxicity of antibiotic-resistant strains of bacteria. Due to the high-strength characteristics and complex recalcitrant pollutants, treatment techniques through combined processes such as anaerobic digestion coupled with advanced oxidation process were found to be more effective than stand-alone methods. Hence, there is need to explore and evaluate innovative treatments and techniques to provide a comprehensive summary of processes that can reduce the toxicity of slaughterhouse wastewater to the environment. This work presents a review of recent studies on the characterization of SWW, innovative treatments and technologies, and critical assessment for future research.

show abstract

“…E. coli, an opportunistic pathogenic bacteria (Kaper et al 2004) causes gastrointestinal infections and other diseases in humans through contact and ingestion. It is widely distributed, and sewage discharge may introduce antibiotic resistance genes (ARGs) and drug-resistant E. coli into the environment (Bibbal et al 2018; Igwaran et al 2018). Thus exploring its abundance and in uencing factors for the treatment of sewage discharge from outlets is of great signi cance (Jang et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Pathogenic bacteria may affectthe bacterial community structure in the sea adjacent to the sewage outlet

Fan

et al. 2022

Preprint

View full text Add to dashboard Cite

Although previous studies have analyzed bacterial diversity and community composition in marine ecosystems, some focused on the relationship between pathogenic bacteria and the composition of bacterial communities in sewage outfalls. The interactions between four pathogenic bacteria (E. coli, Enterococcus, Staphylococcus aureus, fecal coliforms) isolated from the sewage outfall and the bacterial diversity were investigated in the sea adjacent to the Xinghai sewage outlet, China to illustrate the influence of pathogenic bacteria on the bacterial community structure. Higher bacterial diversity was observed in the sewage outlet, and the abundance of pathogenic bacteria and bacterial diversity generally decreased with the increase in the distance from the sewage outlet. Bacterial community structure was mainly influenced by water temperature, conductivity, and dissolved oxygen in summer and winter, while pH, Oxidation-Reduction Potential and salinity were the main factors in autumn. The bacterial abundance had the most significant correlation with E. coli, followed by Enterococcus and fecal coliforms, and the least with Staphylococcus aureus. E. coli (-0.76 < r < 0.79) and Enterococcus (-0.88 < r < 0.77) had similar correlations to the same bacteria, in contrast to Staphylococcus aureus (-0.62 < r < 0.63). The above suggested that pathogenic bacteria may affect the bacterial community structure in the sea adjacent to the sewage outlet besides environmental factors.

show abstract

Mixing of Shiga toxin-producing and enteropathogenic Escherichia coli in a wastewater treatment plant receiving city and slaughterhouse wastewater

Cited by 11 publications

References 54 publications

The First Isolation and Molecular Characterization of Shiga Toxin-Producing Virulent Multi-Drug Resistant Atypical Enteropathogenic Escherichia coli O177 Serogroup From South African Cattle

The First Isolation and Molecular Characterization of Shiga Toxin-Producing Virulent Multi-Drug Resistant Atypical Enteropathogenic Escherichia coli O177 Serogroup From South African Cattle

Characterization of Slaughterhouse Wastewater and Development of Treatment Techniques: A Review

Pathogenic bacteria may affectthe bacterial community structure in the sea adjacent to the sewage outlet

Contact Info

Product

Resources

About