Mixing studies in an unbaffled bioreactor using a computational model corroborated with in-situ Raman and imaging analyses

Vasudevan, Vivek; Eka, Fitriani Nur; Chew, Wee

doi:10.1016/j.ceja.2021.100232

Cited by 5 publications

(9 citation statements)

References 51 publications

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“…The biggest number of previously published papers, which included the colourimetric method for determination of mixing time, regarded stirred tank reactors and bioreactors, including, in some cases, single-use bioreactors [34][35][36]. The investigations included baffled and unbaffled classical tanks agitated with various equipment: Rushton turbines [3,10,[15][16][17]19,24,25,27,28,[31][32][33] and their variants (Scaba SRGT [15]), pitchedblade impellers [3,10,17,24,25,33], disk turbines [19], elephant ear [27,36] and marine blade impellers [27,34,35], hydrofoil [14], Maxblend™ [10,22,23], helical ribbon [13,18,29], a trapezoidal paddle [30], axial flow impellers [24] and planetary [18,21] and coaxial mixers [20]. Some papers contained results from experiments involving orbitally shaken devices, i.e., shake flasks [6], orbitally-shaken single-use bioreactors [37,38,…”

Section: Applications Of the Colourimetric Methods Across Types Of De...mentioning

confidence: 99%

“…Typically, the maximum volume of the vessel analysed using the colourimetric method was no larger than 1000 L. The volume of analysed vessels ranged from the millilitre scale [6,27,30,33,38,44], including vessels from parallel bioreactor systems [35], through bench-scale and laboratory-scale devices [16][17][18][20][21][22]24,[27][28][29]31,32,34,36,37], up to pilotscale systems in the hundred-litre range [14,15,19,22,24,25]. However, one has to note the examples of large-scale reactors, such as the 1500 L orbitally shaken single-use plastic filmbased bag-like container analysed by Tissot et al [37] or the 15 m 3 stirred tank investigated by Rosseburg et al [3].…”

Section: Applications Of the Colourimetric Methods Across Types Of De...mentioning

confidence: 99%

“…The dye does not change its chemical structure during mixing. Examples of dyes that have been used in studies with the colourimetric method are methylene blue [26], Cochineal Red [32], Purple Drimarene R 2 RL [43] and Patent Blue V E131 [45].…”

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confidence: 99%

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The Colourimetric Method for Mixing Time Measurement in Single-Use and Multi-Use Bioreactors—Methodology Overview and Practical Recommendations

Bartczak,

Pilarek

2023

Energies

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Mixing time is an important parameter for quantifying the mixing efficiency of a bioreactor system, essential for successful bioprocess development in various branches of the bioengineering sector (e.g., biopharma, biorefineries, food industry and bioreactor design). The colourimetric method is one of the ways of obtaining valuable quantitative data about the mixing process and the liquid flow inside a vessel. This review consists of a catalogue and a discussion of previously published data in which the colourimetric method has been utilised; a critical comparison between the colourimetric and other mixing time measurement methods; an explanation of practical considerations regarding the bioreactor setup and the choice of reagents; a practical guide for requirements for the acquisition of high-quality images of the mixing process; a thorough discussion of aspects connected to the computer image processing of the video material. This review is intended to thoroughly present the versatility of the colourimetric method for mixing time measurement in miscellaneous bioreactor systems, i.e., in classical tanks and modern single-use (disposable) plastic film-based bag-like containers, and to facilitate the implementation of the colourimetric method in new research setups by providing complete and valuable recommendations about each step of the methodology.

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Section: Applications Of the Colourimetric Methods Across Types Of De...mentioning

confidence: 99%

Section: Applications Of the Colourimetric Methods Across Types Of De...mentioning

confidence: 99%

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The Colourimetric Method for Mixing Time Measurement in Single-Use and Multi-Use Bioreactors—Methodology Overview and Practical Recommendations

Bartczak,

Pilarek

2023

Energies

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“…Working Group for Single-Use Technology are both suitable for validation [19]. However, these are not the only valid approaches, for example, Vivek et al [99] used a Raman spectroscopy probe to experimentally determine the mixing time to validate CFD-determined mixing times.…”

Section: Validationmentioning

confidence: 99%

Computational Fluid Dynamics for Advanced Characterisation of Bioreactors Used in the Biopharmaceutical Industry – Part I: Literature Review

Seidel¹,

Schirmer²,

Maschke³

et al. 2023

Computational Fluid Dynamics - Recent Advances, New Perspectives and Applications

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Computational fluid dynamics (CFD) is a widely used tool for investigating fluid flows in bioreactors. It has been used in the biopharmaceutical industry for years and has established itself as an important tool for process engineering characterisation. As a result, CFD simulations are increasingly being used to complement classical process engineering investigations in the laboratory with spatially and temporally resolved results, or even replace them when laboratory investigations are not possible. Parameters that can be determined include the specific power input, Kolmogorov length, hydrodynamic stress, mixing time, oxygen transfer rate, and for cultivations with microcarriers, the NS1 criterion. In the first part of this series, a literature review illustrates how these parameters can be determined using CFD and how they can be validated experimentally. In addition, an overview of the hardware and software typically used for bioreactor characterisation will also be provided, including process engineering parameter investigations from the literature. In the second part of this series, the authors’ research results will be used to show how the process engineering characterisation of mechanically driven bioreactors for the biopharmaceutical industry (stirred, orbitally shaken, and wave-mixed) can be determined and validated using CFD.

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“…Cells in microfluidic devices are categorically growing in environments different from typical scales in laboratory flasks and bioreactors. Larger cultures at pilot (10 2 -10 3 L) and commercial scales (10 3 -10 5 L) are even more disparate in comparison ( Flickinger, 2010 ; McConville and Kessler, 2010 ; Vivek et al, 2022 ). The metabolomics profiles and culture information acquired via microfluidics experimentation have to be understood within its respective environmental multi-physics considerations.…”

Section: Generation Of Metabolomics Datamentioning

confidence: 99%

Metabolomics and modelling approaches for systems metabolic engineering

Khanijou

Kulyk

Bergès

et al. 2022

Metabolic Engineering Communications

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Mixing studies in an unbaffled bioreactor using a computational model corroborated with in-situ Raman and imaging analyses

Cited by 5 publications

References 51 publications

The Colourimetric Method for Mixing Time Measurement in Single-Use and Multi-Use Bioreactors—Methodology Overview and Practical Recommendations

The Colourimetric Method for Mixing Time Measurement in Single-Use and Multi-Use Bioreactors—Methodology Overview and Practical Recommendations

Computational Fluid Dynamics for Advanced Characterisation of Bioreactors Used in the Biopharmaceutical Industry – Part I: Literature Review

Metabolomics and modelling approaches for systems metabolic engineering

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