Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as organic carbon source

Abstract

Growth parameters and biochemical composition of the green microalga Chlorella vulgaris cultivated under different mixotrophic conditions were determined and compared to those obtained from a photoautotrophic control culture. Mixotrophic microalgae showed higher specific growth rate, final biomass concentration and productivities of lipids, starch and proteins than microalgae cultivated under photoautotrophic conditions. Moreover, supplementation of the inorganic culture medium with hydrolyzed cheese whey powder solution led to a significant improvement in microalgal biomass production and carbohydrate utilization when compared with the culture enriched with a mixture of pure glucose and galactose, due to the presence of growth promoting nutrients in cheese whey. Mixotrophic cultivation of C. vulgaris using the main dairy industry by-product could be considered a feasible alternative to reduce the costs of microalgal biomass production, since it does not require the addition of expensive carbohydrates to the culture medium.

Evaluation of autotrophic and mixotrophic regimen Chlorella pyrenoidosa cells in various wastes water for its biochemical composition and biomass production

“…Hence the result showed that there was some growth promoting factors in dairy waste so as to find higher biomass production than medium containing easily utilizable glucose as sole carbon source. Abreu (Abreu et al, 2012) reported the similar results in which the highest values of X max (3.58 g/L) and P max (0.43 g/L/d) using dairywater as cultivation medium. The results obtained above using the mixotrophic culture where hydrolyzed cheese whey powder solution is used which is comparable to our results.…”

“…Organic carbon sources (as hexoses and pentoses) in dairy wastewater were used to facilitate the sustainability of biofuel industries (Abreu et al, 2012;Kothari et al, 2012;Prajapati et al, 2014;Srinivasan & Subramaniam, 2009;Wang et al, 2009). This capacitates the dairy industry to provide a technology to conserve water, an efficient and simultaneously aggrandize cost of waste water treatment of dairy industries .…”

Effects of Sodium Nitrate and Mixotrophic Culture on Biomass and Lipid Production in Hypersaline Microalgae Dunaliella Viridis Teod

“…In Chlorella vulgaris, mixotrophic culture with 1.0 percent glucose showed the highest lipid productivity compared to other growth conditions (Liang et al 2009). Abreu et al (2012) showed that mixotrophic cultivation of Chlorella vulgaris had higher specific growth rate, final biomass production and also higher lipid productivity. The biomass and lipid production of tested strains in mixotrophic culture were higher compared with other methods of culture especially with marine Chlorella sp.…”

Microalgae as a Feedstock for Biofuel Precursors and Value-Added Products: Green Fuels and Golden Opportunities

“…Supplementation with organic carbon is mostly investigated and applied to boost algal lipid content. But a few studies documented enhancement of carbohydrate or starch content in Chlorella species under mixotrophy or heterotrophy (Abreu et al 2012;Yeh and Chang 2012;Li et al 2015a). In contrast, switching from photoautotrophy did not benefit starch accumulation in C. reinhardtii without stressing the cells (Ball et al 1990).…”