Mixture of different Pseudomonas aeruginosa SD-1 strains in the efficient bioaugmentation for synthetic livestock wastewater treatment

Tang, Huiming; Zhang, Yunyun; Hu, Jingming; Li, Yue; Li, Na; Wang, Meizhen

doi:10.1016/j.chemosphere.2019.124455

Cited by 14 publications

(5 citation statements)

References 45 publications

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“…Dominant microbial phyla and classes in denitrifying systems are well documented, and the findings of this study largely agree, but little literature exists showing correlations between denitrification rates and the relative abundance of specific classes at different temperatures. Shan et al (2023) detailed how bioaugmentation of water resource recovery facilities at low operating temperatures with mixed microorganism consortia leads to the enrichment of functional taxa such as Proteobacteria , Bacteroidota , and Actinobacteria and may represent a potentially useful approach to nitrogen removal under low temperatures. Future work would benefit from an assessment of not only those microbes whose DNA or cellular material are present but also the subset of the microbial community that is active at the time of sampling, for example, through an RNA‐based (Freedman et al, 2015; Romanowicz et al, 2016) or stable isotope‐informed analysis (Piñeiro et al, 2024), which may enable further insight into the association between the abundance and composition of putative denitrifying microorganisms and their functions.…”

Section: Discussionmentioning

confidence: 99%

Impacts of vegetable processing and cheese making effluent on soil microbial functional diversity, community structure, and denitrification potential of land treatment systems

Siemering,

Arriaga,

Cagle

et al. 2024

Water Environment Research

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The cheese making and vegetable processing industries generate immense volumes of high‐nitrogen wastewater that is often treated at rural facilities using land applications. Laboratory incubation results showed denitrification decreased with temperature in industry facility soils but remained high in soils from agricultural sites (75% at 2.1°C). 16S rRNA, phospholipid fatty acid (PLFA), and soil respiration analyses were conducted to investigate potential soil microbiome impacts. Biotic and abiotic system factor correlations showed no clear patterns explaining the divergent denitrification rates. In all three soil types at the phylum level, Actinobacteria, Proteobacteria, and Acidobacteria dominated, whereas at the class level, Nitrososphaeria and Alphaproteobacteria dominated, similar to denitrifying systems such as wetlands, wastewater resource recovery facilities, and wastewater‐irrigated agricultural systems. Results show that potential denitrification drivers vary but lay the foundation to develop a better understanding of the key factors regulating denitrification in land application systems and protect local groundwater supplies.Practitioner Points Incubation study denitrification rates decreased as temperatures decreased, potentially leading to groundwater contamination issues during colder months. The three most dominant phyla for all systems are Actinobacteria, Proteobacteria, and Acidobacteria. The dominant class for all systems is Nitrosphaeria (phyla Crenarchaeota). No correlation patterns between denitrification rates and system biotic and abiotic factors were observed that explained system efficiency differences.

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Section: Discussionmentioning

confidence: 99%

Impacts of vegetable processing and cheese making effluent on soil microbial functional diversity, community structure, and denitrification potential of land treatment systems

Siemering,

Arriaga,

Cagle

et al. 2024

Water Environment Research

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“…In recent years, it has been found that Pseudomonas aeruginosa can reduce nitrate and chromium in wastewater [13]. Other studies have found that Pseudomonas aeruginosa SD-1 has a certain value in the treatment of livestock wastewater [22]. In addition, adding Pseudomonas aeruginosa S5 to coking wastewater can effectively promote the biodegradation of high-weight molecular polycyclic aromatic hydrocarbons in the sludge phase [23,24].…”

Section: Discussionmentioning

confidence: 99%

Genomic and Proteomic Analysis of Pseudomonas aeruginosa Isolated from Industrial Wastewater to Assess Its Resistance to Antibiotics

Wang,

Tian,

Sun

et al. 2023

Separations

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Industrial wastewater usually contains a large amount of organic and inorganic pollutants, and many microorganisms. However, the types of microorganism present in industrial wastewater are still unclear. The aim of this study was to analyze the physicochemical properties and drug resistance of Pseudomonas aeruginosa isolated from industrial wastewater containing high concentrations of sulfate compounds. Pseudomonas aeruginosa was isolated from industrial wastewater from industrial produce with high concentrations of sulfate and phosphate, and mass spectrometry identification, gene identification, biochemical analysis and genomic and proteomic property identification were carried out. According to the results of matrix-assisted flight mass spectrometry and 16S rDNA sequencing, the isolated bacterium was identified as Pseudomonas aeruginosa, and was positive for reactions of ONPG, ACE, GLU, MNE, etc. Through growth experiments, it can be seen that Pseudomonas aeruginosa had a significant growth rate in the LB medium. Antibiotic sensitivity tests showed that Pseudomonas aeruginosa was susceptible to most antibiotics and moderately resistant to Polymyxin B and Polymyxin E. The drug resistance gene experiment showed that Pseudomonas aeruginosa had the gyrB gene related to antibiotic resistance. Proteomic analysis revealed that six proteins were involved in antibiotic resistance. This experiment isolated Pseudomonas aeruginosa from industrial produce wastewater containing high concentrations of sulfate and phosphate ions, providing a new perspective for further research on the characteristics and drug resistance of microorganisms in industrial wastewater and their potential functions when using them to deal with environmental pollution.

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“…Samples were taken out at the designated time points to determine the residual amount of E2 and degradation efficiency. Next, synthetic livestock wastewater was prepared according to the method described by Tang et al [ 52 ]. Degradation tests were performed by adding different concentrations of E2 and concentrations of 4 μg/mL of protein to the wastewater.…”

Section: Methodsmentioning

confidence: 99%

Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

Hao

Pan

et al. 2023

Microb Cell Fact

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Background 17β-estradiol (E2) residues exhibit harmful effects both for human and animals and have got global attention of the scientific community. Microbial enzymes are considered as one of the effective strategies having great potential for removal E2 residues from the environment. However, limited literature is available on the removal of E2 from wastewater using short-chain dehydrogenase. Results In this study, 17β-estradiol degrading enzyme (17β-HSD-0095) was expressed and purified from Microbacterium sp. MZT7. The optimal pH and temperature for reaction was 7 and 40 °C, respectively. Molecular docking studies have shown that the ARG215 residue form a hydrogen bond with oxygen atom of the substrate E2. Likewise, the point mutation results have revealed that the ARG215 residue play an important role in the E2 degradation by 17β-HSD-0095. In addition, 17β-HSD-0095 could remediate E2 contamination in synthetic livestock wastewater. Conclusions These findings offer some fresh perspectives on the molecular process of E2 degradation and the creation of enzyme preparations that can degrade E2. Graphical Abstract

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Mixture of different Pseudomonas aeruginosa SD-1 strains in the efficient bioaugmentation for synthetic livestock wastewater treatment

Cited by 14 publications

References 45 publications

Impacts of vegetable processing and cheese making effluent on soil microbial functional diversity, community structure, and denitrification potential of land treatment systems

Impacts of vegetable processing and cheese making effluent on soil microbial functional diversity, community structure, and denitrification potential of land treatment systems

Genomic and Proteomic Analysis of Pseudomonas aeruginosa Isolated from Industrial Wastewater to Assess Its Resistance to Antibiotics

Characterization of 17β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater

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