Mixture of enzyme-processed Panax ginseng and Gastrodia elata extract prevents UVB-induced decrease of procollagen type 1 and increase of MMP-1 and IL-6 in human dermal fibroblasts

Abstract

According to the previously described anti-photoaging effect of the enzyme-processed Panax ginseng extract and Gastrodia elata extract, we hypothesized that the combination of the two extracts would have superior effect to protect human skin from UVB radiation. Besides, the mixture of active components isolated from herbal extracts, ginsenoside F2, and α-gastrodin was investigated on the photo-protective capability. The expression of aging-related markers including matrix metalloproteinase-1 (MMP-1), interleukin-6 (IL-6), and procollagen type 1 was evaluated using ELISA kits. It was reported that the herbal extract at a Panax ginseng extract to Gastrodia elata extract ratio of 1:10 (w/w) and the compound mixture with equal proportion of ginsenoside F2 and α-gastrodin exhibited significant inhibition of MMP-1 and IL-6 production, and marked upregulation of procollagen type 1 formation. Thus, the combination of either the enzyme-processed herbal extracts or their active components would enhance the properties of prevention and treatment of UVB-induced skin damage.

Effect of anti-skin disorders of ginsenosides- A Systematic Review

“…At the cellular level, F2 significantly reduced MMP-1 expression by 64% and 48% at 1 μg/mL concentration and 10 μg/mL, respectively, in cultured human dermal fibroblasts. However, cytotoxicity was observed at a high dose of 10 μg/mL [ 46 ]. The mixture of 10 μg/mL ginsenoside F2 and 10 μg/mL α-gastrodin exhibited a more substantial anti-aging effect since the expression of MMP-1 and interleukin-6 (IL-6) was significantly inhibited by 18% and 65%, and promoted procollagen type I increased by 200% in UVB-irradiated human dermal keratinocytes [ 47 ].…”

Protective effect of Helianthus annuus seed byproduct extract on ultraviolet radiation‐induced injury in skin cells

“…However, several studies have assessed the effect of UVB radiation on HSFs. [15][16][17][18] In addition, most studies assessing the effect of UVR on HaCaTs have only focused on UVB radiation and not on the combined effect of UVA and UVB radiation. [19][20][21] In this regard, we believe that designing the correct UVR model according to different skin cells will help to obtain more accurate experimental results.…”

“…In contrast, UVA acts on both the epidermis and dermis, and the cells representing the dermis are human skin fibroblasts (HSFs). However, several studies have assessed the effect of UVB radiation on HSFs 15–18 . In addition, most studies assessing the effect of UVR on HaCaTs have only focused on UVB radiation and not on the combined effect of UVA and UVB radiation 19–21 .…”