Mixture toxicity of pyraclostrobine and metiram to the zebrafish (Danio rerio) and its potential mechanism

Wang, Yanhua; Gao, Zhongwen; Liu, Chuande; Mao, Liangang; Liu, Xinju; Ren, Jinjun; Lu, Zeqi; Yao, Jie; Li, Xuan

doi:10.1007/s11356-023-25518-3

Cited by 3 publications

(5 citation statements)

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“…These findings suggested a state of oxidative stress was brought on by acute MET exposure by raising the amount of ROS generation. Similar outcomes in zebrafish were documented [ 21 ]. Additionally, acute MET exposure induced various clinical signs (skin darkness, fin rot, and hemorrhages) and a reduction in fish survivability in a dose-dependent manner.…”

Section: Discussionsupporting

confidence: 83%

“…According to our findings, the 96-hour LC 50 of MET in Nile tilapia was 3.77 mg/L. Similarly, the 96-hour LC 50 of MET was 1.1 mg/L and 85 mg/L in rainbow trout and common carp [ 14 , 22 ], and 0.0025 mg/L in zebrafish [ 21 ]. Susceptibility and physiological differences between fish species might explain the observed variations in the MET 96-hour LC 50 values.…”

Section: Discussionmentioning

confidence: 76%

“…Metiram (MET) is one of many potentially toxic fungicides that enter the environment and endanger fish health. Despite the wide range of MET applications in agricultural practices, there is a lack of studies about the toxicological impacts of this fungicide on a widely and significant culture fish (Nile tilapia) [ 21 , 22 ]. Given this context, our study was designated to better understand the acute negative effects of MET by using Nile tilapia as a model.…”

Section: Discussionmentioning

confidence: 99%

“…Acute toxicity results of MET show that the lethal concentration 50 (LC 50 ) values are 333 µg/L to less than 20,000 µg/L for fish, 110 µg/L to less than 1,000 µg/L for aquatic invertebrates, and 63 µg/L to less than 1,000 µg/L for algae [ 14 ]. MET had severe toxic impacts on zebrafish ( Danio rerio ) embryos including immune-antioxidant alterations and endocrine disruption [ 21 ]. Also, several fish species, including rainbow trout ( Oncorhynchus mykiss ), common carp ( Cyprinus carpio ), sheepshead minnow ( Cyprinodon variegatus ), and bluegill ( Lepomis macrochirus ) were subjected to acute MET toxicological studies [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Toxicological insight of metiram: immuno-oxidative, neuro-behavioral, and hemato-biochemical changes during acute exposure of Nile tilapia (Oreochromis niloticus)

Shaalan,

Elbealy,

Darwish

et al. 2024

BMC Vet Res

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Background The inappropriate use of pesticides including fungicides creates severe biological hazards that can endanger fish health and impede sustainable aquaculture. Objective This study investigated the negative impacts of metiram (MET), a fungicide on the health status of Nile tilapia (Oreochromis niloticus) for a 96-hour duration as an acute exposure in a static renewal system. Methods Three hundred fish (average body weight: 37.50 ± 0.22 g) were assigned into six groups (50 fish/group) with five replicates (10 fish/replicate). Fish were exposed to various six concentrations (0, 1.5, 3, 4.5, 6, and 7.5 mg/L) of MET as a water exposure to for 96-hour without water exchange. The fish’s behavior, clinical signs, and mortalities were documented every day of the exposure period. Additionally, MET’s impact on blood profile, stress biomarkers, hepato-renal functions, immune-antioxidant status, and brain biomarker were closely monitored. Results The lethal concentration (LC50) of MET estimated using Finney’s probit technique was 3.77 mg/L. The fish’s behavior was severely impacted by acute MET exposure, as clear by an increase in surfacing, loss of equilibrium, unusual swimming, laterality, abnormal movement, and a decline in aggressive behaviors. The survivability and hematological indices (white and red blood cell count, differential white blood cell count, hematocrit value, and hemoglobin) were significantly reduced in a concentration-dependent manner following MET exposure. Acute exposure to MET (1.5–7.5 mg/L) incrementally increased stress biomarkers (nor-epinephrine, cortisol, and glucose), lipid peroxides (malondialdehyde), and brain oxidative DNA damage biomarker (8-hydroxy-2-deoxyguanosine). A hepato-renal dysfunction by MET exposure (4.5–7.5 mg/L) was evidenced by the significant increase in the alanine and aspartate aminotransferases and creatinine values. Moreover, a substantial decline in the immune parameters (lysozyme, complement 3, serum bactericidal activity, and antiprotease activity) and antioxidant variables (total antioxidant capacity, superoxide dismutase, and glutathione peroxidase) resulted from acute MET exposure. Conclusion According to these findings, the 96-hour LC50 of MET in Nile tilapia was 3.77 mg/L. MET exposure triggered toxicity in Nile tilapia, as seen by alterations in fish neuro-behaviors, immune-antioxidant status, hepato-renal functioning, and signifying physiological disturbances. This study emphasizes the potential ecological dangers provoked by MET as an environmental contaminant to aquatic systems. However, the long-term MET exposure is still needed to be investigated.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Toxicological insight of metiram: immuno-oxidative, neuro-behavioral, and hemato-biochemical changes during acute exposure of Nile tilapia (Oreochromis niloticus)

Shaalan,

Elbealy,

Darwish

et al. 2024

BMC Vet Res

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“…The toxicity of ZnO nanoparticles in zebra sh is in uenced by several factors, including size, shape, surface charge, and concentration of the nanoparticles. In general, the toxicity of ZnO nanoparticles in zebra sh is similar to that seen in other model organisms and in vitro studies, indicating that zebra sh may be a valuable tool for evaluating the safety of ZnO nanoparticles(Mao et al 2017). However, more research is needed to fully understand the mechanisms of toxicity and the factors that in uence the toxicity of ZnO nanoparticles in zebra sh.…”

mentioning

confidence: 58%

Depolymerization of PET wastes catalysed by Sn and Ag doped ZnO nanoparticles and evaluation of Embryonic Toxicity using Zebrafish

Vinitha¹,

Anbarasu²,

Priya³

et al. 2023

Preprint

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The present investigation focused on the catalytic evaluation of Sn and Ag doped ZnO for depolymerization of polyethylene terephthalate (PET) polyester wastes and embryotoxic evaluation of the doped ZnO nanoparticles using Zebrafish (Danio rerio) embryo model. Bimetallic ZnO NPs doped at 1 and 2 mol% of Sn and Ag synthesized by sol-gel method and characterized by XRD, UV-Vis, FT-IR, SEM-EDS and TEM. Diffraction studies confirmed doped ZnOs retained wurtzite structure as pristine ZnO. However, the electronic spectra showed that the optical bandgap reduced from 3.31 to 3.20 eV upon 2 mol% Sn and Ag doping. SEM-EDS study confirmed elemental composition of Ag and Sn doped ZnO as well as average crystallite size of 60–70 and 115–125 nm for 1 and 2 mol% doped ZnO NPs. Glycolytic and aminolytic depolymerization of PET wastes afforded 90% of bis(2-hydroxyethyl) terephthalate (BHET) and 95% of bis(2-hydroxyethyl) terephthalamide (BHETA) respectively catalysed by 2mol% Sn and Ag doped ZnO nanoparticles. The Sn doped ZnO NPs showed comparatively higher mortality than Ag doped ZnO without any developing deformity in zebrafish embryos.

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New Toxicological Endpoints of Pesticides: Perspectives on Metabolomics

Dong

2023

CAC

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Food safety has received increasing attention, and pesticide contamination is one of the primary issues. Many toxicological endpoints have been established to evaluate the hazards of pesticides. However, the sensitivity of toxic endpoints is dominated by analytical methods. The risks of pesticides may be underestimated when using insensitive analytical methods to establish the toxicological endpoints. Therefore, it is necessary to find highly sensitive analytical approaches to develop a toxicological endpoint. Recently, metabolomics has been widely applied for investigating the toxicological mechanism of environmental pollutants in animals and plants with higher sensitivity. However, metabolomics has not been utilized to establish toxicological endpoints. Herein, the potential of metabolomics for toxicological endpoint establishment is briefly discussed.

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Mixture toxicity of pyraclostrobine and metiram to the zebrafish (Danio rerio) and its potential mechanism

Cited by 3 publications

References 58 publications

Toxicological insight of metiram: immuno-oxidative, neuro-behavioral, and hemato-biochemical changes during acute exposure of Nile tilapia (Oreochromis niloticus)

Toxicological insight of metiram: immuno-oxidative, neuro-behavioral, and hemato-biochemical changes during acute exposure of Nile tilapia (Oreochromis niloticus)

Depolymerization of PET wastes catalysed by Sn and Ag doped ZnO nanoparticles and evaluation of Embryonic Toxicity using Zebrafish

New Toxicological Endpoints of Pesticides: Perspectives on Metabolomics

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