Mixtures of benzo(a)pyrene, dichlorodiphenyltrichloroethane and tributyltin are more toxic to neotropical fish Rhamdia quelen than isolated exposures

Oliveira, Heloísa Helena Paro de; Liebel, Samuel; Rossi, Stéfani Cibele; Azevedo, A.C.B.; Barrera, Ellie A.L.; Garcia, Juan Ramón Esquivel; Grötzner, Sônia Regina; Neto, Francisco Filipak; Randi, Marco Antônio Ferreira; Ribeiro, C.B.

doi:10.1016/j.ecoenv.2015.07.023

Cited by 31 publications

(15 citation statements)

References 73 publications

(82 reference statements)

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“…This result indicates that the BaP concentration used here does not result in measurable neurotoxicity in adult individuals; however, it affects the developing brains of F1 offspring. Acute BaP neurotoxicity in the adult brain manifested as anxiolytic-like behavioral responses in adult zebrafish (waterborne, 7 days; 4 µg/l) [ 94 ] and as increased AChE activity in adult catfish ( Rhamdia quelen ; intraperitoneal injection; 0.3 mg/kg) and adult pale chub ( Zacco platypus ; waterborne; 5 µg/l; 14 days) [ 95 , 96 ]. The aforementioned results indicate that the exposure protocol used in our study is below the threshold to impact neuronal and brain homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish

Wan

et al. 2022

Environmental Epigenetics

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Previous studies have revealed that DNA methylation changes could serve as potential genomic markers for environmental benzo[a]pyrene (BaP) exposure and intergenerational inheritance of various physiological impairments (e.g., obesity and reproductive pathologies). As a typical aromatic hydrocarbon pollutant, direct BaP exposure has been shown to induce neurotoxicity. To unravel the inheritance mechanisms of the BaP-induced bone phenotype in freshwater medaka, we conducted whole-genome bisulfite sequencing of F1 sperm and identified 776 differentially-methylated genes (DMGs). Ingenuity Pathway Analysis (IPA) revealed DMGs were significantly enriched in pathways associated with neuronal development and function. Therefore, it was hypothesized that parental BaP exposure (1 μg/L, 21 days) causes offspring neurotoxicity. Furthermore, the possibility for sperm methylation as an indicator for a neurotoxic phenotype was investigated. The F0 adult brains and F1 larvae were analyzed for BaP-induced direct and inherited toxicity. Acetylcholinesterase activity was significantly reduced in the larvae, together with decreased swimming velocity. Molecular analysis revealed that the marker genes associated with neuron development and growth (alpha1-tubulin, mbp, syn2a, shh, and gap43) as well as brain development (dlx2, otx2, and krox-20) were universally downregulated in the F1 larvae (3-days post-hatching). While parental BaP exposure at an environmentally relevant concentration could induce neurotoxicity in the developing larvae, the brain function of the exposed F0 adults was unaffected. This indicates that developmental neurotoxicity in larvae may result from impaired neuronal development and differentiation, causing delayed brain growth. The present study demonstrates that the possible adverse health effects of BaP in the environment are more extensive than currently understood. Thus, the possibility of multigenerational BaP toxicity should be included in environmental risk assessments.

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Section: Discussionmentioning

confidence: 99%

Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish

Wan

et al. 2022

Environmental Epigenetics

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“…[35,36] Liver histology is a useful tool for the evaluation of the relationship between damage in fish and environmental pollution. [27,[37][38][39] Liver is a key organ for the determination of pollutant actions as it is very sensitive to environmental contaminants, as well as detoxification of endogenous and exogenous products such as pesticides. The hepatocytes are considered prime targets for toxicity of a substance.…”

Section: Discussionmentioning

confidence: 99%

“…[45] The alterations observed here have been detected in other studies with other insecticides, such as fenvalerate in Cirrhinusm rigala, [46] endosulfan in Cichlasoma dimerus, [47] deltamethrin in Oreochromis niloticus, [48] and cypermethrin in Oreochromis mossambicus. [49] Effects of the mix of benzo-apyrene (BaP), dichlorodiphenyltrichloroethane (DDT), and tributyltin (TBT) were evaluated in Rhamdia quelen, [38] suggesting that the presence of these compounds may affect metabolic processes and produce pathological lesions in liver, which is the central organ of detoxification.…”

Section: Discussionmentioning

confidence: 99%

“…Many of these studies related liver damage to increased biochemical biomarkers related to oxidative stress. [38,50,51] Free radicals are important components in the toxic effects of pesticides and other environmental chemicals that induce oxidative stress by interacting with biomolecules. [52] In addition, some cells were found with corresponding lipid storage vacuolization ( Fig.…”

Section: Discussionmentioning

confidence: 99%

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Liver alterations inOreochromis niloticus(Pisces) induced by insecticide imidacloprid: Histopathology and heat shock proteinin situlocalization

Ansoar-Rodríguez

Christofoletti

Correia

et al. 2016

Journal of Environmental Science and Health, Part B

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Liver is very sensitive to environmental contaminants such as pesticides, it being the first target of toxicity of a substance. The objective of this study was to investigate the possible effects of the insecticide imidacloprid (IMI) on the liver of Oreochromis niloticus according concentrations used for growing sugarcane. A semi-quantitative analysis of histopathological alterations of IMI on liver was performed by light microscopy and cellular labeling of heat shock proteins (HSP70) by immunohistochemistry. The most common changes in liver at all concentrations of IMI were hydropic degeneration, pyknotic nuclei, and loss of cell limits. Steatosis and increased levels of HSP70 were detected in hepatocytes with the highest concentration of IMI. In conclusion, the tested concentrations of IMI induced histopathological changes in the liver of O. niloticus and active defence mechanisms to maintain the morphophysiological integrity of the liver. This insecticide has a toxicity potential for these fish, which is a non-target organism of its action.

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“…A ação inibitória de pesticidas organofosforados e carbamatos sobre a atividade desta enzima tem sido reportada por diversos estudos (Galloway et al, 2002;Lionetto et al, 2013;Oliveira et al, 2015).…”

Section: Discussionunclassified

Efeitos biológicos da contaminação sobre bivalves filtradores de regiões do litoral de São Paulo, com ênfase na Baixada Santista

Camargo¹

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Mixtures of benzo(a)pyrene, dichlorodiphenyltrichloroethane and tributyltin are more toxic to neotropical fish Rhamdia quelen than isolated exposures

Cited by 31 publications

References 73 publications

Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish

Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish

Liver alterations inOreochromis niloticus(Pisces) induced by insecticide imidacloprid: Histopathology and heat shock proteinin situlocalization

Efeitos biológicos da contaminação sobre bivalves filtradores de regiões do litoral de São Paulo, com ênfase na Baixada Santista

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