MKL1/miR-5100/CAAP1 loop regulates autophagy and apoptosis in gastric cancer cells

Zhang, Huimin; Li, Hui; Wang, Gen-Xin; Wang, Jun; Yuan, Xiang; Huang, Yingwei; Shen, Chao; Dai, Zhou-Tong; Li, Jiapeng; Zhang, Tongcun; Liao, Xing–Hua

doi:10.1016/j.neo.2020.03.001

Cited by 48 publications

(27 citation statements)

References 44 publications

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“…This study shows that miR-5100 was significantly downregulated in OSCC patients. Zhang et al have suggested that miR-5100 serves as a tumor suppressor in gastric cancer cells [ 47 ]. Similarly, Chijiiwa et al have reported that miR-5100 exerts an inhibitory effect on the occurrence and metastasis of pancreatic cancer [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis

Nakamura

Hiyake

Hamada

et al. 2021

Cancers

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A lack of reliable biomarkers for oral squamous cell carcinoma (OSCC) poses a major clinical issue. The sensitivity and specificity of classical serum tumor markers, such as the squamous cell carcinoma antigen (SCC-Ag), are quite poor, especially for early detection. This study aimed to identify specific serum miRNAs potentially serving as OSCC biomarkers. The expression levels of candidate miRNAs in serum samples from 40 OSCC patients and 40 healthy controls were quantitatively analyzed via microarray and reverse transcription PCR (RT-PCR) analyses. To enhance the accuracy of detection, we used Fisher’s linear discriminant analysis to establish a diagnostic model that incorporated a combination of selected miRNAs. Consequently, miR-19a and miR-20a were significantly upregulated in the patient group (p = 0.014 and 0.036, respectively), whereas miR-5100 was downregulated (p = 0.001). We found that a combination of six miRNAs (miR-24, miR-20a, miR-122, miR-150, miR-4419a, and miR-5100) could distinguish between OSCC and the control group with a higher degree of accuracy (Area Under the Curve, AUC: 0.844, sensitivity: 55%, and specificity: 92.5%). Furthermore, compared to serum SCC antigen, the 6-miRNA panel could accurately detect the presence of OSCC. The present specific miRNAs panel may serve as a novel candidate biomarker of oral cancer.

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Section: Discussionmentioning

confidence: 99%

Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis

Nakamura

Hiyake

Hamada

et al. 2021

Cancers

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“…Autophagy is another programmed cell death that is activated by chrysin in cancer therapy. As autophagy has a dual role in cancer, meaning it may suppress cancer progression, or may function as a pro-survival factor in promoting the proliferation of cancer cells [ 330 , 331 , 332 , 333 ], much attention should be directed towards the regulation of autophagy by chrysin in cancer therapy. It has been reported that chrysin induces autophagy in cancer therapy, showing the anti-tumor role of autophagy.…”

Section: Conclusion and Remarksmentioning

confidence: 99%

Broad-Spectrum Preclinical Antitumor Activity of Chrysin: Current Trends and Future Perspectives

et al. 2020

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Pharmacological profile of phytochemicals has attracted much attention to their use in disease therapy. Since cancer is a major problem for public health with high mortality and morbidity worldwide, experiments have focused on revealing the anti-tumor activity of natural products. Flavonoids comprise a large family of natural products with different categories. Chrysin is a hydroxylated flavonoid belonging to the flavone category. Chrysin has demonstrated great potential in treating different disorders, due to possessing biological and therapeutic activities, such as antioxidant, anti-inflammatory, hepatoprotective, neuroprotective, etc. Over recent years, the anti-tumor activity of chrysin has been investigated, and in the present review, we provide a mechanistic discussion of the inhibitory effect of chrysin on proliferation and invasion of different cancer cells. Molecular pathways, such as Notch1, microRNAs, signal transducer and activator of transcription 3 (STAT3), nuclear factor-kappaB (NF-κB), PI3K/Akt, MAPK, etc., as targets of chrysin are discussed. The efficiency of chrysin in promoting anti-tumor activity of chemotherapeutic agents and suppressing drug resistance is described. Moreover, poor bioavailability, as one of the drawbacks of chrysin, is improved using various nanocarriers, such as micelles, polymeric nanoparticles, etc. This updated review will provide a direction for further studies in evaluating the anti-tumor activity of chrysin.

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“…The expression plasmid pcDNA3.1-STAT3 encoding human STAT3 was constructed in our laboratory and described previously [22]. The promoter areas of miR-15a-5p (-300 to +30) and CX3CL1 (-992 to +19) were augmented by conventional PCR replaced by cloning into pGL3 luciferase reporter vector.…”

Section: Structure Of Luciferase Plasmids and Reporter Assaymentioning

confidence: 99%

“…IL-6 was obtained from Peprotech Inc (Rocky Hill, NJ). The specific steps of the western blotting experiment are as described previously [22,23]. The membranes were visualized by GelDoc XR+ Imaging System (Bio-Rad), examination of the image was examined using the ImageJ software.…”

Section: Antibodies Western Blottingmentioning

confidence: 99%

STAT3/miR-15a-5p/CX3CL1 Loop Regulates Proliferation and Migration of Vascular Endothelial Cells in Atherosclerosis

Li¹,

Zhang²,

Fan³

et al. 2021

Int. J. Med. Sci.

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Endothelial cell proliferation disorder caused by vascular injury seems to be one of the causes of atherosclerosis, which is the pathological basis of coronary heart disease. The role of STAT3 in the regulation of microRNAs and endothelial dysfunction in atherosclerosis is unclear. STAT3 can be activated by cytokine IL-6 and up regulate the expression of CX3CL1. In addition, microRNA-15a-5p (miR-15a-5p) inhibited the transcription of CX3CL1, the proliferation of vascular endothelial cells and the proliferation of STAT3 regulated vascular endothelial cells. STAT3 positively regulates the expression of CX3CL1, and then down-regulates the inhibition of CX3CL1 by over-expression of miR-15a-5p, thus forming an elimination feedback loop to control the proliferation of HUVECs and affect the progression of atherosclerosis. In conclusion, miR-15a-5p may be the therapeutic target of the pathological basis of coronary atherosclerosis.

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MKL1/miR-5100/CAAP1 loop regulates autophagy and apoptosis in gastric cancer cells

Cited by 48 publications

References 44 publications

Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis

Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis

Broad-Spectrum Preclinical Antitumor Activity of Chrysin: Current Trends and Future Perspectives

STAT3/miR-15a-5p/CX3CL1 Loop Regulates Proliferation and Migration of Vascular Endothelial Cells in Atherosclerosis

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