“…Subsequently, the remaining red blood cells were lysed in lysis buffer and enumerated with trypan blue dead cell exclusion dye. Cells were stained with commercially available antibodies, including anti-CD11b Ϫ -allophycocyanin (APC) (clone M1/70, rat IgG2b; Miltenyi Biotec Inc., San Diego, CA, USA), anti-CD11b Ϫ -fluorescein isothiocyanate (clone M1/70, rat IgG2b; Miltenyi Biotec Inc., San Diego, CA, USA), anti-mouse CCR1-APC (clone 643854, rat IgG2b; R&D Systems, Minneapolis, MN, USA), anti-mouse CD195/CCR5-peridinin chlorophyll protein-Alexa Fluor 710 (clone 7A4, Armenian hamster IgG; eBioscience, Inc., San Diego, CA, USA), and anti-mouse CCR2-APC (clone 475301, rat IgG2b; R&D Systems, Minneapolis, MN, USA) following previously described methods (36). Prior to analysis, debris was gated out using forward scatter and side scatter plots, and dead cells were excluded using propidium iodide staining.…”