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Abstract

Serotyping (heat stable antigens) was performed on 398 strains of Campylobacter jejuni from faeces of human enteritis cases in England. Strains isolated over 12 months at three locations were heterogeneous with 33 HS serotypes represented. HS1 and HS4 complex were the predominant types (34% of all strains). The monthly strain frequency distributions were similar at the three locations. The late spring peak appeared to be associated with a rise in miscellaneous serotypes rather than with the emergence of any characteristic predominant serotype. PFGE DNA restriction profiling provided evidence of a high degree of genomic diversity within the common HS1 and HS4 complex serotypes, irrespective of the geographical source, yet some subtypes were common to more than one location. The study showed that C. jejuni strain subtypes from human enteric infections in England were highly diverse, and that HS serotyping must be combined with a more discriminatory subtyping method such as PFGE DNA profiling to provide an accurate basis for epidemiological surveillance.

Campylobacter jejuniMultilocus Sequence Types in Humans, Northwest England, 2003––2004

“…No single sequence type was associated with the late spring seasonal rise in human campylobacteriosis, a finding consistent with that of a previous study in England that used serotyping ( 26 ). However, we have shown complex ST-45 to be significantly more prevalent during summer months in rural than in suburban areas.…”

“…MLST has also provided conceptual advances in understanding the population biology and epidemiology of C. jejuni ( 25 ). Previous studies that used phenotypic strain characterization methods failed to establish source/host associations for particular phenotypes ( 26 ), but recent studies with MLST, including a study in NW England, suggest that a measure of host association may be distinguishable when this system is used ( 27 ). …”

Comparative Genotyping of Campylobacter jejuni Strains from Patients with Guillain-Barré Syndrome in Bangladesh

“…Our PFGE and AFLP data also support those reported in a previous study carried out on Finnish C. jejuni strains [38]. The genetic diversity of C. jejuni is well recognised and is attributed to a number of distinct phenomena, including genomic rearrangements and horizontal gene transfer [39]. A study carried out in England by Owen et al [39] showed that C. jejuni strains from human strains were highly diverse by PFGE analysis.…”

“…The genetic diversity of C. jejuni is well recognised and is attributed to a number of distinct phenomena, including genomic rearrangements and horizontal gene transfer [39]. A study carried out in England by Owen et al [39] showed that C. jejuni strains from human strains were highly diverse by PFGE analysis. Previous studies also described that MLST, AFLP, PFGE and DNA microarrays could not identify GBS-specific genetic markers by comparing the genomes of C. jejuni strains [16], [21], [25], [29].…”