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Mulin, Manorama; Bellio-Spataru, A.

doi:10.1023/a:1006345401325

Cited by 19 publications

(5 citation statements)

References 10 publications

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“…The results are shown in Figure , and the corresponding melting points are 211 °C, 209.7 °C, 206 °C, and 201.9 °C, respectively. Among them, the measured melting point at the heating rate of 2 °C·min –1 is in agreement with that proposed in the literature [(205 to 206) °C]. − The deviations measured at higher heating rate can be explained by the simultaneous occurrence of decomposition during the melting process, which lowers the accuracy of the measured melting point. For example, Figure shows the TG-DSC pattern of TA, and it can be clearly noticed that the decomposition occurred during the melting process at the heating rate of 10 °C·min –1 .…”

Section: Materials and Proceduressupporting

confidence: 89%

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Solid–Liquid Equilibrium of d- and l-Tartaric Acid and Their Importance for Enantioseparation

et al. 2012

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The solid–liquid phase equilibrium of d- and l-tartaric acid (TA) was investigated in this work. Based on the solubility data, the dissolution enthalpy and entropy of the monohydrate racemic tartaric acid (race-TA) and enantiomer were calculated using van't Hoff plots. Binary melting point phase diagrams of d- and l-TA and ternary phase diagrams of d-TA/water/l-TA were also described. The results show that TA exhibits a racemic compound behavior. Furthermore, the influence of temperature on the eutectic points between enantiomer and racemic compounds was investigated. Finally, the importance of solid–liquid equilibrium on the purification and separation of enantiomers was discussed.

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Section: Materials and Proceduressupporting

confidence: 89%

“…Solubility of l -TA in water ●, ref ; ■, ref ; ○, measurement by the static method; ▽, measurement by parallel synthesis.…”

Section: Resultsmentioning

confidence: 99%

Solid–Liquid Equilibrium of d- and l-Tartaric Acid and Their Importance for Enantioseparation

et al. 2012

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“…Since first report by Tran Thanh Van (1973), thin cell layers (TCLs) of plant tissues such as shoots, stem nodes, hypocotyls, etc. have been successfully used as explants for plant regeneration in several plant species such as Lilium longiflorum (Bui et al 1999), Lupinus species (Mulin and Bellio-Spataru 2000), Oryza sativa L. (Nhut et al 2000), Spinacia oleracea (Le'guillon et al 2003) or Brassica napus (Ghnaya et al 2008). The TCL system, consists of explants of a small size (0.1-5 mm) excised from different plant organs either longitudinally or transversally Rout et al 2006), has been applied to shoot regeneration and somatic embryogenesis (Nhut et al 2000;Zhao et al 2007), and was proved to be more efficient than other conventional in vitro culture methods with regard to the total output of plantlets (Lakshmanan et al 1995).…”

Section: Introductionmentioning

confidence: 99%

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

et al. 2009

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An efficient and improved in vitro propagation system for Spilanthes acmella L. using transverse thin cell layer (tTCL) culture system was established. The frequency of shoot regeneration from tTCL nodal segments was affected by concentrations of plant growth regulators and orientation of the explant. MS (Murashige and Skoog in Physiol Plant 15:473-497, 1962) medium with 5.0 mg dm -3 BAP was optimal for shoot regeneration. Upon this medium, the explant inoculated in the upright orientation exhibited a high frequency of shoot regeneration (about 97%), and the highest number of shoots (31.5) per explant. The intact node (1.0-1.5 cm) cultured on the same medium had significantly lower shoot multiplication ability with only 4.5 shoots per responsive explant. As compared to BAP alone, the combination of BAP and Kin or NAA did not have positive effects on shoot multiplication from tTCL nodal segments. Rooting of shoots was achieved on growth regulator free full-strength MS medium. Plantlets were transplanted into soil with 90-100% survival rate.

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“…Through tTCLs technology, micropropagation could be obtained in recalcitrant (Mulin and Bellio-Spataru 2000;Leguillon et al 2003) medicinal (Ahn et al 1996) and other crop plant species (Baskaran et al 2006;Ben Ghnaya et al 2008) in rapid multiplication. Moreover, tissue culture technology provides means of mass propagation of a species of interest with profound potential economic spin-offs (Teixeira da Silva 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture

Baskaran

Jayabalan

2009

Acta Physiol Plant

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An effective protocol was developed for in vitro propagation of Psoralea corylifolia via somatic embryogenesis in cell suspension culture. Embryogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 6 lM naphthaleneacetic acid (NAA) and 30 lM glutamine from transverse TCLs from 10-day-old hypocotyl explants with a 96.4% frequency. Embryogenic callus produced a higher number of somatic embryos (123.7 ± 1.24 per gram fresh weight callus) on MS medium containing 30 g l -1 sucrose, 1 lM NAA, 4 lM benzyladenine (BA), 15 lM glutamine and 2 lM abscisic acid (ABA) after 4 weeks of culture. Somatic embryos successfully germinated (97.6%) on MS medium containing 20 g l -1 sucrose, 8 g l -1 agar and supplemented with 2 lM BA, 1 lM ABA and 2 lM gibberellic acid (GA 3 ) within 2 weeks of culture. Somatic embryos developed into normal plants, which hardened with 100% efficiency in soil in a growth chamber. Plants were successfully transferred to greenhouse and subsequently established in the field. Plant survival percentage in the field differed with seasonal variations. Average psoralen content of 12.9 lg g -1 DW was measured in different stages of somatic embryo development by high-performance liquid chromatography (HPLC). This protocol will be helpful for efficient propagation of elite clones on a mass scale, conservation efforts of this species and for secondary metabolites production studies.

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Cited by 19 publications

References 10 publications

Solid–Liquid Equilibrium of d- and l-Tartaric Acid and Their Importance for Enantioseparation

Solid–Liquid Equilibrium of d- and l-Tartaric Acid and Their Importance for Enantioseparation

An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture

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