MMP-3 and MMP-8 in rat mandibular condylar cartilage associated with dietary loading, estrogen level, and aging

Yu, Jia; Mursu, Eerika; Typpö, Matleena; Laaksonen, Sakari; Voipio, Hanna‐Marja; Pesonen, Paula; Raustia, Aune; Pirttiniemi, Pertti

doi:10.1016/j.archoralbio.2018.10.037

Cited by 12 publications

(15 citation statements)

References 46 publications

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“…Studies have confirmed that MMP-3 is the most important hydrolase, which has a strong ability to activate various MMP precursors, gelatinase, and collagenase to degrade the extracellular matrix of cartilage and bone [30], and its increasing level is positively correlated with the degree of cartilage destruction [31,32]. In our study, LIPUS effectively reduced MMP-3 expression levels in damaged condylar cartilage caused by CSD treatment.…”

Section: Discussionsupporting

confidence: 73%

“…The LIPUS 30, 45, and 60 group rats received the LIPUS intervention at the corresponding intensity for 20 min daily [15] under isoflurane gas anesthesia while simultaneously undergoing CSD. The bilateral temporomandibular joint intervention was performed using the OSTEOTRON IV ultrasound equipment (Ito Ultrawave Co., Ltd., Tokyo, Japan), the ultrasound intensity of which includes three levels (30,45, and 60 mW/cm 2 ): the ultrasound frequency was 1.0 MHz, the pulse width was 200 μs, and the repetition rate was 1 kHz.…”

Section: Csd and Lipusmentioning

confidence: 99%

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The Optimal Regimen for the Treatment of Temporomandibular Joint Injury Using Low-Intensity Pulsed Ultrasound in Rats with Chronic Sleep Deprivation

Liang

Yang

et al. 2020

BioMed Research International

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Low-intensity pulsed ultrasound (LIPUS) is an emerging physical therapy for the treatment of early temporomandibular joint injury and has a good effect on promoting cartilage and subchondral bone tissue repair. However, the best LIPUS intensity and treatment duration remain unclear. This study is aimed at observing the preventive and therapeutic effects of different modes of LIPUS and at identifying the optimal LIPUS treatment regimen for temporomandibular joint injury. In the present study, rat models of temporomandibular joint injury were established using a chronic sleep deprivation (CSD) method, and the effect of LIPUS as intensities of 30, 45, and 60 mW/cm2 was observed at 7, 14, and 21 days. After CSD, the condylar cartilage of the rats demonstrated variable degrees of surface roughening, collagen fiber disarrangement or even partial exfoliation, decreased proteoglycan synthesis and cartilage thickness, decreased chondrocyte proliferation, decreased type 2 collagen (COL-2) expression, and increased matrix metalloproteinase- (MMP-) 3 expression at all three time points. When the rats with CSD received different intensities of LIPUS treatment, the pathological changes were alleviated to various extents. The groups receiving 45 mW/cm2 LIPUS showed the most significant relief of cartilage damage, and this significant effect was observed on days 14 and 21. These results demonstrated that LIPUS can effectively inhibit CSD-induced condylar cartilage damage in rats, and LIPUS treatment at an intensity of 45 mW/cm2 for at least 2 weeks is the optimal regimen for temporomandibular joint injury.

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Section: Discussionsupporting

confidence: 73%

Section: Csd and Lipusmentioning

confidence: 99%

The Optimal Regimen for the Treatment of Temporomandibular Joint Injury Using Low-Intensity Pulsed Ultrasound in Rats with Chronic Sleep Deprivation

Liang

Yang

et al. 2020

BioMed Research International

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“…The thickness of MCC has been demonstrated to be relied on the mechanical loading, which had a great effect on proliferation. 15 - 17 Compared with the normal diet group, MCC harvested from the soft diet group was thinner. The significant difference was mainly found in anterior and posterior part of MCC, which is similar to Rusu et al’s 18 findings.…”

Section: Discussionmentioning

confidence: 97%

“…The anterior part of MCC was suggested to be sensitive to mechanical loading. 17 , 19 Even though the thickness of middle part was growing, we considered that the stress sensibility of middle part was not as strong as the other two parts, because of the smaller number of BrdU-positive cells.…”

Section: Discussionmentioning

confidence: 99%

The effect induced by alternated mechanical loading on Notch-1 in mandibular condylar cartilage of growing rabbits

Fan

Feng

Yang

et al. 2021

Bone & Joint Research

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Aims The aim of our study is to investigate the effect induced by alternated mechanical loading on Notch-1 in mandibular condylar cartilage (MCC) of growing rabbits. Methods A total of 64 ten-day-old rabbits were randomly divided into two groups according to dietary hardness: normal diet group (pellet) and soft diet group (powder). In each group, the rabbits were further divided into four subgroups by feeding time: two weeks, four weeks, six weeks, and eight weeks. Animals would be injected 5-bromo-2′-deoxyuridine (BrdU) every day for one week before sacrificing. Histomorphometric analysis of MCC thickness was performed through haematoxylin and eosin (HE) staining. Immunochemical analysis was done to test BrdU and Notch-1. The quantitative real-time polymerase chain reaction (qRT-PCR) and western blot were used to measure expression of Notch-1, Jagged-1, and Delta-like 1 (Dll-1). Results The thickness of MCC in the soft diet group was thinner than the one in normal diet group. Notch-1 was restricted in fibrous layer, proliferative layer, and hypertrophic layer. The expression of Notch-1 increased from two weeks to six weeks and then fell down. Notch-1 in normal diet group was higher than that in soft diet group in anterior part of MCC. The statistical differences of Notch-1 were shown at two, four, and six weeks (p < 0.05). The result of western blot and quantitative real-time PCR (qRT-PCR) showed the expression of Dll-1 and Jagged-1 rose from two to four weeks and started to decrease at four weeks. BrdU distributed in all layers of cartilage and subchondral bone. The number of BrdU-positive cells, which were less in soft diet group, was decreasing along with the experiment period. The significant difference was found at four, six, and eight weeks in anterior and posterior parts (p < 0.05). Conclusion The structure and proliferation of MCC in rabbits were sensitive to dietary loading changes. The proper mechanical loading was essential for transduction of Notch signalling pathway and development of mandibular condylar cartilage. Cite this article: Bone Joint Res 2021;10(7):437–444.

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“…Various biomarkers of aging/disease have been associated with biological functions ( Supplementary Table S5 ). For example, MMP8, the top aging marker, is a collagenase with proteolytic activity against matrix proteins and is an important participant in the function of the vascular system and the development of atherosclerosis ( Laxton et al, 2009 ; Toba et al, 2017 ; Yu et al, 2019 ). Its expression in the heart increases with chronological age, enabling it to regulate signal transduction during the aging process by regulating the expression and activity of cytokines, chemokines, growth factors, hormones, and angiogenic factors ( Mota et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

The Inflamm-Aging Model Identifies Key Risk Factors in Atherosclerosis

Chen

Yao

et al. 2022

Front. Genet.

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Background: Atherosclerosis, one of the main threats to human life and health, is driven by abnormal inflammation (i.e., chronic inflammation or oxidative stress) during accelerated aging. Many studies have shown that inflamm-aging exerts a significant impact on the occurrence of atherosclerosis, particularly by inducing an immune homeostasis imbalance. However, the potential mechanism by which inflamm-aging induces atherosclerosis needs to be studied more thoroughly, and there is currently a lack of powerful prediction models.Methods: First, an improved inflamm-aging prediction model was constructed by integrating aging, inflammation, and disease markers with the help of machine learning methods; then, inflamm-aging scores were calculated. In addition, the causal relationship between aging and disease was identified using Mendelian randomization. A series of risk factors were also identified by causal analysis, sensitivity analysis, and network analysis.Results: Our results revealed an accelerated inflamm-aging pattern in atherosclerosis and suggested a causal relationship between inflamm-aging and atherosclerosis. Mechanisms involving inflammation, nutritional balance, vascular homeostasis, and oxidative stress were found to be driving factors of atherosclerosis in the context of inflamm-aging.Conclusion: In summary, we developed a model integrating crucial risk factors in inflamm-aging and atherosclerosis. Our computation pipeline could be used to explore potential mechanisms of related diseases.

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MMP-3 and MMP-8 in rat mandibular condylar cartilage associated with dietary loading, estrogen level, and aging

Cited by 12 publications

References 46 publications

The Optimal Regimen for the Treatment of Temporomandibular Joint Injury Using Low-Intensity Pulsed Ultrasound in Rats with Chronic Sleep Deprivation

The Optimal Regimen for the Treatment of Temporomandibular Joint Injury Using Low-Intensity Pulsed Ultrasound in Rats with Chronic Sleep Deprivation

The effect induced by alternated mechanical loading on Notch-1 in mandibular condylar cartilage of growing rabbits

The Inflamm-Aging Model Identifies Key Risk Factors in Atherosclerosis

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