MMP-8, MMP-9 and Neutrophil Elastase in Peripheral Blood and Exhaled Breath Condensate in COPD

Sng, JieHao Joshua; Prazáková, Silvie; Thomas, Paul S.; Herbert, Cristan

doi:10.1080/15412555.2016.1249790

Cited by 44 publications

(32 citation statements)

References 42 publications

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“…Reportedly, juvenile MMP8‐deficient mice exhibited increased mortality, which seemed to result from reduced bacterial clearance due to reduced phagocytosis and diminished NET efficiency . MMP8 is also known to play an important role in the transport of leucocytes in chronic and acute inflammation; for example, increased expression of MMP8 is detected in the PBMCs of COPD patients with acute exacerbation (AECOPD) . Recent studies have shown that the expression of MMP8 is upregulated in the blood of patients with sepsis through transcriptomic analysis .…”

Section: Discussionmentioning

confidence: 99%

“…30 MMP8 is also known to play an important role in the transport of leucocytes in chronic and acute inflammation; for example, increased expression of MMP8 is detected in the PBMCs of COPD patients with acute exacerbation (AECOPD). 31 Recent studies have shown that the expression of MMP8 is upregulated in the blood of patients with sepsis through transcriptomic analysis. 32 In the present study, we found that the expression of MMP8 in PBMCs in the early stage of sepsis was significantly higher than that in non-septic ICU patients and increased even further in patients with a septic shock.…”

Section: F I G U R E 8 Five Proteins From the Intersection: Non-septimentioning

confidence: 99%

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Data‐independent acquisition‐based quantitative proteomic analysis reveals differences in host immune response of peripheral blood mononuclear cells to sepsis

Tong

et al. 2019

Scand J Immunol

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This study was aimed to uncover proteins that are differentially expressed in sepsis. Data‐independent acquisition (DIA) was used for analysis to identify differentially expressed proteins in peripheral blood mononuclear cells (PBMCs) of patients. A total of 24 non‐septic intensive care unit (ICU) patients, 11 septic shock patients and 27 patients diagnosed with sepsis were recruited for the mass spectrometry (MS) discovery. PBMCs were isolated from routine blood samples and digested into peptides. A DIA workflow was developed using a quadrupole‐Orbitrap liquid chromatography LC‐MS system, and mass spectra peaks were extracted by Skyline software. Orthogonal partial least‐squares discriminant analysis (OPLS‐DA) and partial least‐squares discriminant analysis (PLS‐DA) were applied to distinguish the patient groups at the level of fragment ion and peptide. Differentially expressed proteins in the patient groups were verified by enzyme‐linked immunosorbent assay (ELISA). Receiver‐operating characteristic (ROC) curves were used to evaluate the protein expression. A total of 1062 fragment ions and 122 proteins were identified in the MS‐DIA analysis conducted by Skyline software. Using gene ontology clustering analysis, we discovered that 51 of the 122 identified proteins were associated with biological processes, including carbon metabolism, biosynthesis of antibiotics, platelet activation, bacterial invasion of epithelial cells and complement, and coagulation cascades. Among them, five proteins (high‐mobility group box1 [HMGB1], matrix metalloproteinase 8 [MMP8], neutrophil gelatinase‐associated lipocalin [NGAL], lactotransferrin [LTF] and grancalcin [GCA]) were identified by ELISA as closely related to the development of sepsis. The ROC curves displayed good sensitivity and specificity.

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Section: Discussionmentioning

confidence: 99%

Section: F I G U R E 8 Five Proteins From the Intersection: Non-septimentioning

confidence: 99%

Data‐independent acquisition‐based quantitative proteomic analysis reveals differences in host immune response of peripheral blood mononuclear cells to sepsis

Tong

et al. 2019

Scand J Immunol

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“…Microbial overload in the airways could also induce the release of myeloperoxidase from neutrophils, generate cell debris and reactive oxygen species (ROS), and aggravate damage to pulmonary structures. 56,57 In response to DAMPs, PRRs such as TLRs on neutrophils in peripheral blood and lung tissue are also over-expressed, thereby amplify neutrophil recruitment and activation. 36,58 Excessive infiltration of neutrophils in the lungs leads to the release of MMPs and neutrophil elastase (stored in azurophilic granules), which mediate a protease-antiprotease imbalance in the lung.…”

Section: Neutrophilsmentioning

confidence: 99%

<p>How Do Innate Immune Cells Contribute to Airway Remodeling in COPD Progression?</p>

Bu¹,

Wang²,

Yin³

2020

COPD

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Recently, the therapeutic potential of immune-modulation during the progression of chronic obstructive pulmonary disease (COPD) has been attracting increasing interest. However, chronic inflammatory response has been oversimplified in descriptions of the mechanism of COPD progression. As a form of first-line airway defense, epithelial cells exhibit phenotypic alteration, and participate in epithelial layer disorganization, mucus hypersecretion, and extracellular matrix deposition. Dendritic cells (DCs) exhibit attenuated antigen-presenting capacity in patients with advanced COPD. Immature DCs migrate into small airways, where they promote a pro-inflammatory microenvironment and bacterial colonization. In response to damageassociated molecular patterns (DAMPs) in lung tissue affected by COPD, neutrophils are excessively recruited and activated, where they promote a proteolytic microenvironment and fibrotic repair in small airways. Macrophages exhibit decreased phagocytosis in the large airways, while they demonstrate high pro-inflammatory potential in the small airways, and mediate alveolar destruction and chronic airway inflammation. Natural killer T (NKT) cells, eosinophils, and mast cells also play supplementary roles in COPD progression; however, their cellular activities are not yet entirely clear. Overall, during COPD progression, "exhausted" innate immune responses can be observed in the large airways. On the other hand, the innate immune response is enhanced in the small airways. Approaches that inhibit the inflammatory cascade, chemotaxis, or the activation of inflammatory cells could possibly delay the progression of airway remodeling in COPD, and may thus have potential clinical significance.

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“…Of the 2 studies on MMP -9 in EBC from patients with COPD, one showed no correlation between MMP -9 and spirometry parameters (either in stable disease or exacerbation), 8 while in the other, such an analysis could not have been performed because MMP--9 was not found in EBC from any of the study participants. 20 We found lower total MMP -9 concentrations in patients with COPD who were treated with ICS when compared to those who were steroid -naive. Such a difference was not documented when active MMP -9 was compared; moreover, there was DISCUSSION Our study is one of the very few reports on MMP -9 concentrations in EBC in patients with COPD.…”

Section: Matrix Metalloproteinase 9 Levels and Functional And Clinicamentioning

confidence: 62%

Exhaled matrix metalloproteinase 9 in patients with stable COPD - an observational study

Maskey-Warzęchowska¹,

Górska²,

Nejman-Gryz³

et al. 2018

Polish Archives of Internal Medicine

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Introduction Data on the measurement of matrix metalloproteinase 9 (MMP‑9) in exhaled breath condensate (EBC) from patients with chronic obstructive pulmonary disease (COPD) are scarce and inconsistent. Objectives We aimed to assess the usefulness of enzyme‑linked immunosorbent assay (ELISA) and immunoenzymatic assay (IEA) for the measurement of MMP‑9 in EBC, the agreement between the results of both methods, and the relationships between total and active MMP‑9 in EBC and clinical and functional COPD characteristics. Patients and methods Total (ELISA and IEA) and active (IEA) MMP‑9 levels were assessed in EBC from 70 patients with stable COPD and 21 controls and correlated with pulmonary function and COPD symptom severity. Results MMP‑9 levels did not reach the sensitivity threshold of the ELISA kit in any of the COPD patients and in 11 controls. Total and active MMP‑9 (IEA) levels did not differ between COPD patients and controls. In COPD patients, total MMP‑9 levels correlated positively with forced expiratory volume in 1 second (FEV1) and FEV1 to forced vital capacity ratio and inversely with residual volume to total lung capacity ratio. A weak positive correlation between active MMP‑9 concentrations and COPD Assessment Test (CAT) score was found (r = 0.31, P = 0.01). Conclusions The utility of ELISA in MMP‑9 assessment in EBC is limited in COPD patients, while MMP‑9 measurement in EBC by IEA is feasible. The positive correlation between active MMP‑9 and CAT score in our patients and the inverse relationship between total MMP‑9 concentration and the degree of airway obstruction reflect the complex role of MMP‑9 in COPD.

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MMP-8, MMP-9 and Neutrophil Elastase in Peripheral Blood and Exhaled Breath Condensate in COPD

Cited by 44 publications

References 42 publications

Data‐independent acquisition‐based quantitative proteomic analysis reveals differences in host immune response of peripheral blood mononuclear cells to sepsis

Data‐independent acquisition‐based quantitative proteomic analysis reveals differences in host immune response of peripheral blood mononuclear cells to sepsis

<p>How Do Innate Immune Cells Contribute to Airway Remodeling in COPD Progression?</p>

Exhaled matrix metalloproteinase 9 in patients with stable COPD - an observational study

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