Mn-doped ZnS quantum dots/methyl violet nanohybrids for room temperature phosphorescence sensing of DNA

Abstract

Mn-doped ZnS quantum dots /methyl violet nanohybrids were explored to develop a novel room temperature phosphorescence (RTP) sensor for the detection of DNA. Methyl violet (MV) as the electron acceptors was adsorbed on the surface of the quantum dots (QDs) to quench the RTP of the Mn-doped ZnS QDs through an electron-transfer process under excitation. The addition of DNA recovered the RTP signal of the Mn-doped ZnS QDs due to the binding of MV with DNA and the removal of MV from the surface of the Mn-doped ZnS QDs. Under the optimal conditions, the enhanced RTP intensity of the Mn-doped ZnS QDs/MV nanohybrids linearly increased with the concentration of DNA from 0.08 to 12 mg L −1 with the detection limit of 33.6 μg L −1 . The relative standard deviation for eleven replicate detections of the reagent blank was 3.7%. The developed method was applied to the detection of DNA in spiked urine samples with recoveries of 96%−103% without interference from nonspecific fluorescence. room temperature phosphorescence, quantum dots, photoinduced electron transfer, sensor, DNA

Doped quantum dots for chemo/biosensing and bioimaging

“…The electrostatic interaction of methyl violet and MPA-capped Mn-doped ZnS QDs allows the design of an RTP assay for turn-on sensing of DNA. 251 Upon electrostatic assembling with Mn-doped ZnS QDs, the phosphorescence of Mn-doped ZnS QDs is quenched by methyl violet. In the presence of double-strand DNA, methyl violet detaches from the surface of Mn-doped ZnS QDs by intercalating into the grooves of double-strand DNA, thereby the phosphorescence of Mn-doped ZnS QDs is recovered, giving a detection limit of 34 mg L À1 for double-strand DNA.…”

Phosphorescent quantum dots/ethidium bromide nanohybrids based on photoinduced electron transfer for DNA detection

“…For systems without DNA and with 0.2 mg L À1 DNA, the 11 continuous parallel detections on phosphorescence intensity had a relative standard deviation (RSD) of 1.7%. The detection limit of this method is similar to that of other nanoparticle nonspecific DNA quantitative determination methods [10]. But the system has a wider linear range and the RTP suffers from less background interference from biological fluids, so this system can detect DNA content in complex biological fluids without complex pretreatments.…”

“…PIET-based mechanism has been speculated to rely on the electrostatic attraction between the quencher and the surface groups of QDs as a result of luminescent quenching, and the emission restoration by a receptor could bind with the quencher and remove it from the surface of QDs [9]. Previous studies are based on the fluorescence properties of QDs [10][11][12][13][14]. However, photoinduced electron transfer (PIET) sensors based on room temperature phosphorescence (RTP) of QDs have been rarely reported.…”

A novel phosphorescence sensor for Co2+ ion based on Mn‐doped ZnS quantum dots

“…Tu et al (22) synthesized amine-capped Mn-doped ZnS nanocrystals for fluorescence analysis. Yan group (23)(24)(25)(26) extended such nanocrystals to RTP sensing DNA, persistent organic pollutants in water, enoxacin and glucose in biological fluids. Wu and Fan (27) also reported doped ZnS QDs as phosphorescence sensors for detecting raceanisodamie hydrochloride and atropine sulfate in biological fluids.…”