Mn3O4 nanoparticles: Synthesis, characterization and their antimicrobial and anticancer activity against A549 and MCF-7 cell lines

Abstract

Due to their inexpensive and eco-friendly nature, and existence of manganese in various oxidation states and their natural abundance have attained significant attention for the formation of Mn 3 O 4 nanoparticles (Mn 3 O 4 NPs). Herein, we report the preparation of Mn 3 O 4 nanoparticles using manganese nitrate as a precursor material by utilization of a precipitation technique. The as-prepared Mn 3 O 4 nanoparticles (Mn 3 O 4 NPs) were characterized by using X-ray powder diffraction (XRD), UV–Visible spectroscopy (UV–Vis), High-Resolution Transmission electron microscopy (HRTEM), Field emission scanning electron microscopy (FESEM), Thermal gravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FT-IR). The antimicrobial properties of the as-synthesized Mn 3 O 4 nanoparticles were investigated against numerous bacterial and fungal strains including S. aureus, E. coli , B. subtilis, P. aeruginosa, A. flavus and C. albicans . The Mn 3 O 4 NPs inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 40 μg/ml and C. albicans with a MIC of 15 μg/ml. Furthermore, the Mn 3 O 4 NPs anti-cancer activity was examined using MTT essay against A549 lung and MCF-7 breast cancer cell lines. The Mn 3 O 4 NPs revealed significant activity against the examined cancer cell lines A549 and MCF-7. The IC 50 values of Mn 3 O 4 NPs with A549 cell line was found at concentration of 98 µg/mL and MCF-7 cell line was found at concentration of 25 µg/mL.

Toxicology assessment of manganese oxide nanomaterials with enhanced electrochemical properties using human in vitro models representing different exposure routes

“…The obtained results (viability determination and oxidative stress formation) showed a dose-dependent cytotoxicity within the employed concentration range (1-10 mg L -1 ). These results are in accordance with the work of Shaik et al, who showed as well a decrease in cell viability on the range of concentrations over 10-25 mg L -1 in response to Mn3O4 nanoparticles exposure (Shaik et al, 2021). Interestingly, manganese oxide NMs coated with polyethylene glycol (PEG) have shown very low toxicity, in terms of cell viability reduction, towards the same cell line, in a higher concentration range (200-1000 mg L -1 ) (Zhan et al, 2017).…”

Toxicology assessment of manganese oxide nanomaterials with enhanced electrochemical properties using human in vitro models representing different exposure routes

“…The obtained results (viability determination and ROS formation) showed a dose-dependent cytotoxicity within the employed concentration range (1–10 mg L −1 ). These results are in accordance with the work of Shaik et al who showed as well a decrease in cell viability on the range of concentrations over 10–25 mg L −1 in response to Mn 3 O 4 nanoparticles exposure 19 . Interestingly, manganese oxide NMs coated with polyethylene glycol (PEG) have shown very low toxicity, in terms of cell viability reduction, towards the same cell line, in a higher concentration range (200–1000 mg L −1 ) 20 .…”

Anti-breast Adenocarcinoma and Anti-urease Anti-tyrosinase Properties of 5-Pentylresorcinol as Natural Compound with Molecular Docking Studies

“…The absorbance values obtained from the control wells were averaged and this value was evaluated as 100％ cell viability. The absorbance values obtained from the compound treated wells were proportioned to the control absorbance value and the percent viability values were calculated 23) .…”