MnASI1 Mediates Resistance to Botrytis cinerea in Mulberry (Morus notabilis)

Wang, Donghao; Gong, Na; Liu, Chaorui; Li, Suxia; Guo, Zhancheng; Wang, Gefan; Shang, Qiqi; Wang, Dongming; Ji, Xiaodong; Xin, Youchao

doi:10.3390/ijms232113372

Cited by 9 publications

(5 citation statements)

References 38 publications

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“…Reduced antioxidant enzyme activity after treatment of pathogen mycelium with crude extracts of fermentation broth, leading to the accumulation of ROS, can cause destruction of pathogen cells [ 39 ]. Therefore, as oxidative damage intensifies, the cell membrane system is disrupted and the level of malondialdehyde (MDA), one of the main products of the oxidation of lipid substances in the membrane system, increases [ 40 ]. In our results, after treatment of B. sorokiniana mycelium with different levels of crude extracts of strain JK-25, the mycelial antioxidant enzyme activity was significantly reduced compared to the control and the MDA content was significantly increased, which is consistent with previous studies.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Kang

Niu

Zhang

et al. 2023

Plants

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Common root rot caused by Bipolaris sorokiniana infestation in wheat is one of the main reasons for yield reduction in wheat crops worldwide. The bacterium strain JK-25 used in the current investigation was isolated from wheat rhizosphere soil and was later identified as Bacillus halotolerans based on its morphological, physiological, biochemical, and molecular properties. The strain showed significant antagonism to B. sorokiniana, Fusarium oxysporum, Fusarium graminearum, and Rhizoctonia zeae. Inhibition of B. sorokiniana mycelial dry weight and spore germination rate by JK-25 fermentation supernatant reached 60% and 88%, respectively. The crude extract of JK-25 was found, by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), to contain the surfactin that exerted an inhibitory effect on B. sorokiniana. The disruption of mycelial cell membranes was observed under laser scanning confocal microscope (LSCM) after treatment of B. sorokiniana mycelium with the crude extract. The antioxidant enzyme activity of B. sorokiniana was significantly reduced and the oxidation product malondialdehyde (MDA) content increased after treatment with the crude extract. The incidence of root rot was significantly reduced in pot experiments with the addition of JK-25 culture fermentation supernatant, which had a significant biological control effect of 72.06%. Its ability to produce siderophores may help to promote wheat growth and the production of proteases and pectinases may also be part of the strain’s role in suppressing pathogens. These results demonstrate the excellent antagonistic effect of JK-25 against B. sorokiniana and suggest that this strain has great potential as a resource for biological control of wheat root rot strains.

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Section: Discussionmentioning

confidence: 99%

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Kang

Niu

Zhang

et al. 2023

Plants

View full text Add to dashboard Cite

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“…Reduced antioxidant enzyme activity after treatment of pathogen mycelium with crude extracts of fermentation broth, leading to accumulation of ROS and thus destruction of pathogen cells [39]. Therefore, as oxidative damage intensifies, the cell membrane system is disrupted and the level of malondialdehyde (MDA), one of the main products of the oxidation of lipid substances in the membrane system, increases [40]. In our results, after treatment of B. sorokiniana mycelium with different levels of crude extracts of strain JK-25, the mycelial antioxidant enzyme activity was significantly reduced compared to the control and the MDA content was significantly increased, which is consistent with previous studies.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Strain <em>Bacillus Halotolerans</em> JK-25 Mediate the Biocontrol of Wheat Common Root Rot Caused by <em>Bipolaris Sorokiniana </em>

Kang¹,

Zhang²,

Niu³

et al. 2023

Preprint

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Common root rot caused by Bipolaris sorokiniana infestation of wheat is one of the main reasons of yield reduction in wheat crops worldwide. In current study, strain JK-25 was isolated from soil of wheat rhizosphere and identified as Bacillus halotolerans based on morphological, physiological, biochemical characteristics and molecular identification. The strain showed significant antagonism to B.sorokiniana and broad-spectrum resistance to Fusarium oxysporum, Fusarium graminearum and Rhizoctonia zeae. Inhibition of Bipolaris sorokiniana mycelial dry weight and spore germination rate by JK-25 fermentation supernatant reached 60% and 88% respectively. The crude extract of JK-25 was found by MALDI-TOF-MS to contain the surfactin that exerted an inhibitory effect on B.sorokiniana. The disruption of mycelial cell membranes was observed under microscopy (LSCM) after treatment of B.sorokiniana mycelium with the crude extract. The antioxidant enzyme activity of B.sorokiniana was significantly reduced and the oxidation product MDA content increased after treatment with the crude extract. The incidence of root rot was significantly reduced in pot experiments with the addition of JK-25 culture ferment, which had a significant biological control effect of 72.06%. Its ability to produce siderophores may help to promote wheat growth, and the production of proteases and pectinases may also be part of the strain's role in suppressing pathogens. These results demonstrate the excellent antagonistic effect of JK-25 against B.sorokiniana and suggest that this strain has great potential as a resource for biological control of wheat root rot strains.

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“…It can infect more than 200 economically important crops worldwide (Williamson et al 2007, Dean et al 2012, AbuQamar et al 2017). Meanwhile, it stands as one of the primary fungal diseases affecting mulberry plants (Xin et al 2020, Wang et al 2022). There are few studies on the mechanism of plant miRNAs in response to B. cinerea , especially in woody plants.…”

Section: Introductionmentioning

confidence: 99%

Mno‐miR164a and MnNAC100 regulate the resistance of mulberry to Botrytis cinerea

Wang,

Chen,

Liu

et al. 2024

Physiologia Plantarum

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Although microRNAs (miRNAs) regulate the defense response of a variety of plant species against a variety of pathogenic fungi, the involvement of miRNAs in mulberry's defense against Botrytis cinerea has not yet been documented. In this study, we identified responsive B. cinerea miRNA mno‐miR164a in mulberry trees. After infection with B. cinerea, the expression of mno‐miR164a was reduced, which was fully correlated with the upregulation of its target gene, MnNAC100, responsible for encoding a transcription factor. By using transient infiltration/VIGS mulberry that overexpressed mno‐miR164a or knocked‐down MnNAC100, our study revealed a substantial enhancement in mulberry's resistance to B. cinerea when mno‐miR164a was overexpressed or MnNAC100 expression was suppressed. This enhancement was accompanied by increased catalase (CAT) activity and reduced malondialdehyde (MDA) content. In addition, mno‐miR164a‐mediated inhibition of MnNAC100 enhanced the expression of a cluster of defense‐related genes in transgenic plants upon exposure to B. cinerea. Meanwhile, MnNAC100 acts as a transcriptional repressor, directly suppressing the expression of MnPDF1.2. Our study indicated that the mno‐miR164a‐MnNAC100 regulatory module manipulates the defense response of mulberry to B. cinerea infection. This discovery has great potential in breeding of resistant varieties and disease control.

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MnASI1 Mediates Resistance to Botrytis cinerea in Mulberry (Morus notabilis)

Cited by 9 publications

References 38 publications

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Antagonistic Strain Bacillus halotolerans Jk-25 Mediates the Biocontrol of Wheat Common Root Rot Caused by Bipolaris sorokiniana

Antagonistic Strain <em>Bacillus Halotolerans</em> JK-25 Mediate the Biocontrol of Wheat Common Root Rot Caused by <em>Bipolaris Sorokiniana </em>

Mno‐miR164a and MnNAC100 regulate the resistance of mulberry to Botrytis cinerea

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