Virtual Reality (VR) has not yet been exploited in the assessment of spatial memory. Current VR systems for the assessment of spatial memory include interaction by using traditional devices (e.g., computer screens, mouses or keyboards). This classical type of interaction with the system is ineffective because the user's sense of presence is very poor. If the user does not have the feeling of "being there" in the virtual environment, the spatial memory may not be assessed correctly. The user's physical movement can contribute to a high level of presence. Therefore, by developing a system that allows the participants to become active in a virtual environment, spatial memory can be assessed as the same way it could be evaluated in a real environment. Firstly, I would like to express my sincere gratitude to my supervisors Prof. M. Carmen Juan and Dr. Magdalena Méndez López for the continuous support of my Ph.D. thesis and related research, for their patience, motivation, and immense knowledge. Their guidance helped me during the realization of this Ph.D. I would also like to extend my thanks to my lab mates of the