MnSOD mediates shear stress-promoted tumor cell migration and adhesion

Ma, Shijun; Fu, Afu; Lim, Sierin; Chiew, Geraldine Giap Ying; Luo, Kathy Qian

doi:10.1016/j.freeradbiomed.2018.09.004

Cited by 23 publications

(19 citation statements)

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“…MnSOD level was significantly higher in tumor tissues compared to matched control ( Figure 4 ), which is in agreement with other studies reporting on breast cancer patients ( 34 ). Several studies have reported that overexpression of MnSOD positively regulates cancer progression from a localized to an invasive phenotype ( 35 , 36 ) and tumor cell adhesion ( 37 , 38 ) in a panel of breast cancer cells. In vivo and in vitro studies demonstrated Nrf2-driven MnSOD upregulation postulated from degradation of Caveolin-1 resulted in enhanced cell survival, metastasis, and drug resistance in circulating breast cancer cells ( 39 , 40 ).…”

Section: Discussionmentioning

confidence: 99%

Expression of Autophagy and Mitophagy Markers in Breast Cancer Tissues

et al. 2021

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Mitochondria play important roles in regulating cell bioenergetics status and reactive oxygen species (ROS) generation. ROS-induced mitochondrial damage is among the main intracellular signal inducers of autophagy. Autophagy is a cellular catabolic process that regulates protein and organelle turnover, while a selective form of autophagy, mitophagy, specifically targets dysfunctional mitochondrial degradation. This study aims to measure the levels of autophagy, mitophagy, oxidative stress, and apoptosis in invasive breast carcinoma tissues using immunohistochemistry (IHC). Tissue microarrays of 76 patients with breast cancer were stained with six IHC markers (MnSOD, Beclin-1, LC3, BNIP3, Parkin, and cleaved caspase 3). The expression intensity was determined for each tumor tissue and the adjacent tumor-matched control tissues. Intermediate and strong staining scores of MnSOD, Beclin-1, LC-3, BNIP-3, and Parkin were significantly higher in tumor tissues compared to the adjacent matched control. The scoring intensity was further classified into tissues with negative staining and positive staining, which showed that positive scores of Beclin-1 and Parkin were significantly high in tumor tissues compared to other markers. Positive association was also noted between BNIP-3 and Beclin-1 as well as LC-3 and cleaved caspase-3 immunostaining. To our knowledge, this is one of the first studies that measure both mitophagy and autophagy in the same breast cancer tissues and the adjacent matched control. The findings from this study will be of great potential in identifying new cancer biomarkers and inspire significant interest in applying anti-autophagy therapies as a possible treatment for breast cancer.

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Section: Discussionmentioning

confidence: 99%

Expression of Autophagy and Mitophagy Markers in Breast Cancer Tissues

et al. 2021

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“…An in vitro Transwell assay was conducted to investigate the deformability of cancer cells as reported. [ 56 ] In brief, MCF7, HeLa and MDA‐MB‐231 cell lines were collected from culture dish by trypsin. After quantify the cell density, cells were washed once with serum free medium and resuspended in serum free medium at a density of 1 × 105 cells per mL.…”

Section: Methodsmentioning

confidence: 99%

Active Particle Based Selective Transport and Release of Cell Organelles and Mechanical Probing of a Single Nucleus

Yossifon

2020

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Self‐propelling micromotors are emerging as a promising microscale tool for single‐cell analysis. The authors have recently shown that the field gradients necessary to manipulate matter via dielectrophoresis can be induced at the surface of a polarizable active (“self‐propelling”) metallo‐dielectric Janus particle (JP) under an externally applied electric field, acting essentially as a mobile floating microelectrode. Here, the application of the mobile floating microelectrode to trap and transport cell organelles in a selective and releasable manner is successfully extended. This selectivity is driven by the different dielectrophoretic (DEP) potential wells on the JP surface that is controlled by the frequency of the electric field, along with the hydrodynamic shearing and size of the trapped organelles. Such selective and directed loading enables purification of targeted organelles of interest from a mixed biological sample while their dynamic release enables their harvesting for further analysis such as gene/RNA sequencing or proteomics. Moreover, the electro‐deformation of the trapped nucleus is shown to be in correlation with the DEP force and hence, can act as a promising label‐free biomechanical marker. Hence, the active carrier constitutes an important and novel ex vivo platform for manipulation and mechanical probing of subcellular components of potential for single cell analysis.

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“…Afterwards, the RISC uses its RNase activity to cleave the target RNA [14]. RNAi technology has been widely used to silence gene expression in human cells in the last twenty years [15][16][17][18]. The past several years have witnessed the rapid growth of RNAi-based clinical trials for viral infections such as Epstein-Barr virus (EBV) [19], hepatitis B virus (HBV) [20], human immunodeficiency virus (HIV) [21], SARS-CoV [22] and the raging SARS-CoV-2 [13].…”

Section: Ivyspringmentioning

confidence: 99%

Developing effective siRNAs to reduce the expression of key viral genes of COVID-19

Wu¹,

Luo²

2021

Int. J. Biol. Sci.

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The COVID-19 pandemic has been raging worldwide for more than a year. Many efforts have been made to create vaccines and develop new antiviral drugs to cope with the disease. Here, we propose the application of short interfering RNAs (siRNAs) to degrade the viral genome, thus reducing viral infection. By introducing the concept of the probability of binding efficiency (PBE) and combining the secondary structures of RNA molecules, we designed 11 siRNAs that target the consensus regions of three key viral genes: the spike (S), nucleocapsid (N) and membrane (M) genes of SARS-CoV-2. The silencing efficiencies of the siRNAs were determined in human lung and endothelial cells overexpressing these viral genes. The results suggested that most of the siRNAs could significantly reduce the expression of the viral genes with inhibition rates above 50% in 24 hours. This work not only provides a strategy for designing potentially effective siRNAs against target genes but also validates several potent siRNAs that can be used in the clinical development of preventative medication for COVID-19 in the future.

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MnSOD mediates shear stress-promoted tumor cell migration and adhesion

Cited by 23 publications

References 50 publications

Expression of Autophagy and Mitophagy Markers in Breast Cancer Tissues

Expression of Autophagy and Mitophagy Markers in Breast Cancer Tissues

Active Particle Based Selective Transport and Release of Cell Organelles and Mechanical Probing of a Single Nucleus

Developing effective siRNAs to reduce the expression of key viral genes of COVID-19

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