Mobile origami immunosensors for the rapid detection of urinary tract infections

Adrover-Jaume, Cristina; Rojo-Molinero, Estrella; Clemente, Antonio; Russell, Steven M.; Arranz, Javier; Oliver, Antonio; Rica, Roberto de la

doi:10.1039/d0an01218a

Cited by 17 publications

(13 citation statements)

References 29 publications

(37 reference statements)

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“…This approach has been used to develop immunosensors made solely of paper that detect pathogens and biomarkers with ultrafast immunoreaction steps of only 5 min. [89][90][91] It has also been used to deliver nanoparticles to other materials with entrapped analytes. For example, pressing the reservoirs against surgical face masks worn by COVID-19 patients enabled the detection of viral antigens trapped in the polypropylene layer.…”

Section: Reservoirsmentioning

confidence: 99%

“…Reagents reservoir to detect LDH [84] Rolling cercle amplification (RCA) reagents encapsulation [85] Pullulan/trehalose Enzymes and DNAzymes to detect E. coli [86] CMC Store GOX and HRP to detect glucose in urine [87] PSS Store and release antibody decorated gold nanoparticles [88][89][90][91][92] Figure 5. Examples of reservoirs for paper-based biosensors.…”

Section: Pullulanmentioning

confidence: 99%

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Polymer Components for Paper‐Based Analytical Devices

Campo

Vaquer

Rica

2022

Adv Materials Technologies

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In recent years, paper has become an essential substrate material for developing different types of sensors, from wearable devices to single‐use test strips and biosensors. In parallel, a polymer‐based toolbox has emerged in order to add additional functions to these paper‐based analytical devices. In this article, examples are compiled from the recent literature showing microfluidic systems based on printing impermeable polymer barriers with different methods as well as the implementation of electrochemical, fluorescent, and colorimetric polymer‐based transducers in paper‐based analytical platforms. Externally actuated or dissolvable polymer valves and reservoirs, analyte concentrators, and separation devices, as well as polymer‐based recognition elements (molecularly imprinted polymers) printed on paper substrates are also reviewed. The search has revealed a plethora of possibilities for developing complex lab‐on‐chip devices implementing different polymer‐based components in them. The ability of patterning these polymers with common printing methods that do not require specialized facilities paves the way for mass producing this kind of advanced paper‐based analytical device.

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Section: Reservoirsmentioning

confidence: 99%

Section: Pullulanmentioning

confidence: 99%

Polymer Components for Paper‐Based Analytical Devices

Campo

Vaquer

Rica

2022

Adv Materials Technologies

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“…A mobile origami sensor was developed by Adrover-Jaume et al [102] capable of detecting UTIs caused by Escherichia coli in less than 7 min. A single piece of paper containing antibody-decorated Au NPs was made from an origami nanosensor.…”

Section: Diagnosis Of Utis By Nanotechnologymentioning

confidence: 99%

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

et al. 2021

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The diagnosis and treatment of urinary tract infections (UTIs) remain challenging due to the lack of convenient assessment techniques and to the resistance to conventional antimicrobial therapy, showing the need for novel approaches to address such problems. In this regard, nanotechnology has a strong potential for both the diagnosis and therapy of UTIs via controlled delivery of antimicrobials upon stable, effective and sustained drug release. On one side, nanoscience allowed the production of various nanomaterial-based evaluation tools as precise, effective, and rapid procedures for the identification of UTIs. On the other side, nanotechnology brought tremendous breakthroughs for the treatment of UTIs based on the use of metallic nanoparticles (NPs) for instance, owing to the antimicrobial properties of metals, or of surface-tailored nanocarriers, allowing to overcome multidrug-resistance and prevent biofilm formation via targeted drug delivery to desired sites of action and preventing the development of cytotoxic processes in healthy cells. The goal of the current study is therefore to present the newest developments for the diagnosis and treatment of UTIs based on nanotechnology procedures in relation to the currently available techniques.

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“…Likewise, gold nanoclusters have also been employed in the detection of pathogenic bacteria via plasmon enhanced fluorescence . In another study, antibody linked Au NPs have been applied for the designing of an origami platform for the facile detection of a bacterial infection . Apart from nanoparticle based detection, various nanostructured SERS substrates have also been studied for the diagnosis of UTIs targeting both bacterial ,− and genomic , detection pathways.…”

Section: Introductionmentioning

confidence: 99%

“…50 In another study, antibody linked Au NPs have been applied for the designing of an origami platform for the facile detection of a bacterial infection. 51 Apart from nanoparticle based detection, various nanostructured SERS substrates have also been studied for the diagnosis of UTIs targeting both bacterial 45,52−54 and genomic 55,56 detection pathways. However, a very minimal focus has been made toward pathogen detection targeting UTIs based on optoplasmonic metal nanotwins.…”

Section: Introductionmentioning

confidence: 99%

Point-of-Care Biosensing of Urinary Tract Infections Employing Optoplasmonic Surfaces Embedded with Metal Nanotwins

Basak

Mitra

Gogoi

et al. 2022

ACS Appl. Bio Mater.

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We report the synthesis of gold nanotwins (Au NTs) on a solid and transparent glass substrate which in turn has been employed for the selective optoplasmonic detection of Escherichia coli (EC) bacteria in human urine for the point-of-care diagnosis of urinary tract infections (UTIs). As compared to the single nanoparticle systems (Au NPs), the Au NTs show an enriched localized surface plasmon resonance (LSPR) due to the enhancement of the electric field under electromagnetic irradiation, e.g., photon, which helps in improving the limits of detection. For this purpose, initially a simple glass surface has been coated with Au NPs, with the help of the linker 3-aminopropyl-triethoxysilane – APTES. The surface has been linked further with another Au NP with the help of the 1,10-alkane-dithiol linker with two thiol ends, which eventually leads to the development of the optoplasmonic surface with Au NTs and an enhanced LSPR response. Subsequently, the EC specific aptamer has been chemically immobilized on the surface of Au NTs with the blocking of free sites via bovine serum albumin (BSA). Remarkably, Raman spectroscopy unfolds a 7-fold increase in the peak intensities with the Au NTs on the glass surface as compared to the surface coated with isolated Au NPs. The enhancement in the LSPR response of glass substrates coated with Au NTs and the EC specific aptamer has been further utilized for the selective and sensitive detection of UTIs. The results have been verified with the help of UV–visible spectroscopy to establish the utility of the proposed sensing methodology. An extensive interference study with other bacterial species unveils the selectivity and specificity of the proposed optoplasmonic sensors toward EC with a detection range of 5 × 103 to 107 CFU/mL. Intuitively, the method is more versatile in a sense that the sensor can be made specific to any other pathogens by simply changing the design of the aptamer. Finally, a low-cost, portable, and point-of-care optoplasmonic transduction setup is designed with a laser light illumination source, a sample holder, and a sensitive photodetector for the detection of UTIs in human urine.

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Mobile origami immunosensors for the rapid detection of urinary tract infections

Abstract: Immunosensors made of paper interfaced with smartphones diagnose UTIs in less than 10 minutes.

Cited by 17 publications

References 29 publications

Polymer Components for Paper‐Based Analytical Devices

Polymer Components for Paper‐Based Analytical Devices

Nanomaterials for the Diagnosis and Treatment of Urinary Tract Infections

Point-of-Care Biosensing of Urinary Tract Infections Employing Optoplasmonic Surfaces Embedded with Metal Nanotwins

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