Mobile phone radiation causes changes in gene and protein expression in human endothelial cell lines and the response seems to be genome‐ and proteome‐dependent

Nylund, R; Leszczyński, D

doi:10.1002/pmic.200600076

Cited by 93 publications

(72 citation statements)

References 20 publications

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“…We have previously determined that the 900 MHz GSM mobile phone radiation signal alters expression of several tens of proteins in the human endothelial cell line EA.hy926 (Leszczynski, et al, 2002;Nylund and Leszczynski, 2004;Nylund and Leszczynski, 2006). In the present study we have examined whether the 1800 MHz GSM mobile phone radiation signal exposure will also affect protein expression in EA.hy926 cells.…”

Section: Introductionmentioning

confidence: 92%

Proteomic Analysis of the Response of Human Endothelial Cell Line EA.hy926 to 1800 GSM Mobile Phone Radiation

Nylund¹,

H²,

Kuster³

et al. 2009

J Proteomics Bioinform

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Background:We have earlier shown that exposure of human endothelial cell line EA.hy926 to 900 MHz GSM mobile phone radiation causes changes in the expression of numerous proteins. Here, we have examined the effects of 1800 MHz GSM mobile phone signal on the proteome of the same cell line.Results: EA.hy926 cells were exposed for one hour to 1800 MHz GSM signal, simulating mobile phone talking conditions, at an average specific absorption rate (SAR) of 2.0 W/kg at 37±0.3°C. Sham samples were produced simultaneously in the same conditions but without the radiation exposure. Cells were harvested immediately after 1-hour exposure to the radiation, and proteins were extracted and separated using 2-dimensional electrophoresis (2DE). In total, 10 experimental replicates were generated from both exposed and sham samples. About 900 protein spots were detected in the 2DE-gels using PDQuest software and eight of them were found to be differentially expressed in exposed cells (p<0.05, t-test). Three out of these eight proteins were identified using Maldi-ToF mass spectrometry (MS). These proteins are: spermidine synthase (SRM), 78 kDa glucose-regulated protein (55 kDa fragment) (GRP78) and proteasome subunit alpha type 1 (PSA1). Due to the lack of the availability of commercial antibodies we were able to further examine expression of only GRP78. Using SDS-PAGE and western blot method we were not able to confirm the result obtained for GRP78 using 2DE. Additionally, we have not seen any effect of 1800GSM exposure on the expression of vimentin and Hsp27 -proteins that were affected by the 900 MHz GSM exposure in our earlier studies. Conclusions:Our results suggest that the 900GSM and 1800GSM exposures might affect the expression of some proteins in the EA.hy926 cell line. The observed here discrepancy between the expression changes of GRP78 detected with 1DE and 2DE confirms the importance of validation of the results obtained with 2DE using other methods, e.g. western blot.

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Section: Introductionmentioning

confidence: 92%

Proteomic Analysis of the Response of Human Endothelial Cell Line EA.hy926 to 1800 GSM Mobile Phone Radiation

Nylund¹,

H²,

Kuster³

et al. 2009

J Proteomics Bioinform

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“…Experimental data obtained from our study indicated that 1800 MHz of EMR exposure for 6 weeks (2 hrs. /day) resulted in mutation in BRCA1 and TP53 genes [ [31] found that EMR caused different effects on c-Fos gene expression in the rat brain, Nylund & Leszezynski [32] showed that gene and protein expression were altered, in two variants of human endothelial cell line, in response to one hour mobile phone radiation exposure. Karien, et al [33] showed that the EMR alters protein expression in human endothelial cell line.…”

Section: Discussionmentioning

confidence: 99%

Adverse Effect of Mobile Phone on Tp53, Brcai Genes and Dna Fragmentation in Albino Rat Liver

Em¹,

Mk²,

Sa³

2013

Int J Genom Proteomics

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“…For example, Lee et al (2005) used the serial analysis of gene expression (SAGE) technique and found that 2.45 GHz RF radiation at a 10-W/kg SAR for 2∼6 h affected the expression of 221 genes, including apoptosis-related genes and cell cycle genes, in human HL-60 cells without a significant increase of HSP expression. Nylund et al (2006) reported that two variants of a human endothelial cell line responded differently to 900 MHz GSM signal mobile phone radiation (Nylund et al, 2006). With regard to the effect of RF exposure to rat brain, Belyaev et al (2006) reported that GSM at 915 MHz induced no detectable DNA double-stranded breaks or changes in chromatin conformation but affected the expression of genes related to neurotransmitter regulation, blood brain barrier, and melatonin production in rat brain cells.…”

Section: Introductionmentioning

confidence: 99%

“…Although most studies on the biological effects of RF exposure could not detect any molecular changes, some reports demonstrated RF-specific gene expression (Belyaev et al, 2006;Lee et al, 2005;Nylund et al, 2006). For example, Lee et al (2005) used the serial analysis of gene expression (SAGE) technique and found that 2.45 GHz RF radiation at a 10-W/kg SAR for 2∼6 h affected the expression of 221 genes, including apoptosis-related genes and cell cycle genes, in human HL-60 cells without a significant increase of HSP expression.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Response of Normal WI-38 Human Fibroblast Cells to 1,763 MHz Radiofrequency Radiation

Im¹,

Kim²,

Park³

et al. 2010

Genomics & Informatics

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Increased exposure of human to RF fields has raised concerns for its potential adverse effects on our health. To address the biological effects of RF radiation, we used genome wide gene expression as the indicator. We exposed normal WI-38 human fibroblast cells to 1763 MHz mobile phone RF radiation at a specific absorption rate (SAR) of 60 W/kg with an operating cooling system for 24 h. There were no alterations in cell numbers or morphology after RF exposure. Through microarray analysis, we identified no differentially expressed genes (DEGs) at the 0.05 significance level after controlling for multiple testing errors with the Benjaminiochberg false discovery rate (BH FDR) method. Meanwhile, 82 genes were differentially expressed between RF-exposed cells and controls when the significance level was set at 0.01 without correction for multiple comparisons. We found that 24 genes (0.08% of the total genes examined) were changed by more than 1.5-fold on RF exposure. However, significant enrichment of any gene set or pathway was not observed from the functional annotation analysis. From these results, we did not find any evidence that non-thermal RF radiation at a 60-W/kg SAR significantly affects cell proliferation or gene expression in WI-38 cells.

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Mobile phone radiation causes changes in gene and protein expression in human endothelial cell lines and the response seems to be genome‐ and proteome‐dependent

Cited by 93 publications

References 20 publications

Proteomic Analysis of the Response of Human Endothelial Cell Line EA.hy926 to 1800 GSM Mobile Phone Radiation

Proteomic Analysis of the Response of Human Endothelial Cell Line EA.hy926 to 1800 GSM Mobile Phone Radiation

Adverse Effect of Mobile Phone on Tp53, Brcai Genes and Dna Fragmentation in Albino Rat Liver

Genome-wide Response of Normal WI-38 Human Fibroblast Cells to 1,763 MHz Radiofrequency Radiation

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