The leakage of gases and chemical vapors is a common accident in laboratory processes that requires a rapid response to avoid harmful effects if humans and instruments are exposed to this leakage. In this paper, the performance of a portable sensor node designed for integration with mobile and stationary robots used to transport chemical samples in automated laboratories was tested and evaluated. The sensor node has four main layers for executing several functions, such as power management, control and data preprocessing, sensing gases and environmental parameters, and communication and data transmission. The responses of three metal oxide semiconductor sensors, BME680, ENS160, and SGP41, integrated into the sensing layer have been recorded for various volumes of selected chemicals and volatile organic compounds, including ammonia, pentane, tetrahydrofuran, butanol, phenol, xylene, benzene, ethanol, methanol, acetone, toluene, and isopropanol. For mobile applications, the sensor node was attached to a sample holder on a mobile robot (ASTI ProBOT L). In addition, the sensor nodes were positioned close to automation systems, including stationary robots. The experimental results revealed that the tested sensors have a different response to the tested volumes and can be used efficiently for hazardous gas leakage detection and monitoring.