Please cite this article as: S. Luz, M. Masoodian, R.R. Cesario, M. Cesario, Using a serious game to promote community-based awareness and prevention of neglected tropical diseases, Entertainment Computing (2015), doi: http://dx.doi.org/10.1016/j.entcom. 2015.11.001 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Article title: Using a serious game to promote community-based awareness and prevention of neglected tropical diseases A serious game is presented which embodies an approach to disease prevention and management that is community-based and incorporates principles of the One Health framework---a broader view of healthcare that encompasses people, animals and the environment. The game focuses specifically on two related neglected and emerging infectious tropical diseases, namely Visceral Leishmaniasis (VL) and American Cutaneous Leishmaniasis (ACL). It seeks to inform and encourage changes in behaviours and attitudes in local populations, while involving multidisciplinary teams of healthcare professionals and researchers. The design of the learning outcomes, gameplay, client interface, and architecture of the game are discussed in detail, along with the process of collaboration among a team of computer scientists, designers, medical researchers, and practitioners which underpinned the development of the game. An expert study conducted to gather feedback from a multidisciplinary group of participants comprising medical researchers, veterinarians, healthcare service administrators, and community healthcare providers is presented which forms part of our initial assessment of this approach. The results of this study and their implications for the design of similar health-related serious games are also discussed.Using a serious game to promote community-based awareness and prevention of neglected tropical diseases