Mobilisation of satellite cells following ischaemia and reperfusion in primate skeletal muscle

Gregory, M.A.; Mars, Maurice

doi:10.17159/2078-516x/2004/v16i1a189

Cited by 8 publications

(4 citation statements)

References 44 publications

(65 reference statements)

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“…Also activated satellite cells were observed with increased volume together with ribosomes in cytoplasm [41] . This finding was in agreement with other research [42] . Remarkably, it was reported that PRP produces a more pronounced increase of myogenic precursor cells together with an expansion of the myogenic cell pool necessary for myofiber formation [30] .…”

Section: Discussionsupporting

confidence: 94%

Histological Study of the Effect of Platelet Rich Plasma on Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats

Badr

Elbakary

Laag

et al. 2019

Egyptian Journal of Histology

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Background: Muscle injuries are common and may be associated with impaired functional capacity, especially among athletes. Ordinary lines of treatment result in improper healing of the damaged tissue with prolonged time for recovery, so it was essential to try new lines of treatment for these injuries to get advantageous effect on the healing process. One of these new treatments is platelet-rich plasma (PRP), which is used to improve healing of many lesions such as skin and sports injury. Aim of the work: This work was carried out to evaluate the role of platelet rich plasma in healing of experimentally induced skeletal muscle injury in adult male albino rat. Materials and methods: Thirty adult male albino rats were divided Into: Group I: donor group to obtain PRP from their peripheral blood and muscle specimens from their gasterocnemius muscle. Group II: was exposed to bilateral gastrocnemius muscles injury and was left without treatment. Group III: Bilateral gastrocnemius muscles were injured and immediately treated locally with PRP intramuscularly. Muscle specimens were excised after 1, 7and 21 days from the onset of injury and were processed for light and electron microscopic examination. Results: PRP treated group demonstrated early formation of many myotubes starting from the 7th day after injury onwards in association with ultrastructural features of activated satellite cells together with restoration of normal histological features of muscle fibers. Conclusion: local injection of PRP into the injured gastrocnemius muscle resulted in early activation, proliferation and differentiation of satellite cells resulting in earlier and enhanced myogenesis.

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Section: Discussionsupporting

confidence: 94%

Histological Study of the Effect of Platelet Rich Plasma on Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats

Badr

Elbakary

Laag

et al. 2019

Egyptian Journal of Histology

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“…Such activity prevents exit from the cell cycle which is required for cellular differentiation [41]. The process of myogenesis in adult ischaemic tissue may be incomplete as while satellite cells increase in number, these are generally not activated cells and cannot achieve maturity or differentiation [42]. Further work to elucidate the pathway that activates and causes the proliferation of quiescent satellite cells into myoblasts or renews the population of these active myogenic precursors would be the next logical step.…”

Section: Discussionmentioning

confidence: 99%

Critical Limb Ischaemia in Adult Human Skeletal Muscle Increases Satellite Cell Proliferation but Not Differentiation

Hart¹,

Tsui²,

Khan³

et al. 2015

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Critical Limb Ischaemia (CLI) is defined as the presence of rest pain, ulcers and/or gangrene in a limb for over 2 weeks. Associated exercise intolerance is caused by muscle fibre atrophy, fibrofatty infiltration, nerve dysfunction, mitochondrial damage and myofibril disorder. We aimed to determine the behaviour of satellite cells, responsible for the repair and regeneration of damaged muscle, in repairing the damage caused to critically ischaemic adult human skeletal muscle. CD34, pax7 and MyoD are all markers of satellite cells at different stages of the cell cycle and allow for an assessment of their number and activity in ischaemia. Local ethical committee approval and informed consent was obtained. Samples of harvested gastrocnemius muscle of patients undergoing major perigenicular amputation for CLI (n = 10) were analysed and compared to a control group undergoing coronary artery bypass grafting (n = 10). Using immunohistochemistry, the expression of pax7, CD34 and MyoD was assessed in five sequential blinded randomly generated fields. Statistical testing of the data collected was made via the Mann Whitney U test. Protein electrophoresis was used to confirm overall protein expression of the satellite cell markers. There was a significant increase in the number of satellite cells observed in CLI muscle sections as demonstrated by the expression of pax7 (2.4× fold p < 0.0001). CD34 expressing Haematopoietic Stem Cells (HSCs) and satellite cells were also more abundant, with a 2× fold increase observed (p < 0.0001) whilst those cells expressing both CD34 and pax7 and identified as quiescent satellite cells, were significantly greater in number in the CLI samples (2.9× fold p < 0.0001), confirmed via immunohistochemistry and protein electrophoresis. There was a significant decrease in the expression of MyoD positive or activated satellite cells (p < 0.0001). This indicates an increase in the proliferation of the satellite cell population as a response to CLI but less active cells are observed.

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“…On the periphery of this severely damaged region (level 4) are myofibres that, whilst damaged, are still supplied with a limited blood supply. These myofibres may be repaired and remodelled by satellite cell or sarcolemmal fusion (Gregory & Mars 2004). The central region of the muscle is essentially cushioned from the crush component of the contusion and the vascular bed remains largely intact.…”

Section: Discussionmentioning

confidence: 99%

The creation of a measurable contusion injury in skeletal muscle

Deane¹,

Gregory²,

Mars³

2014

J S Afr Vet Assoc

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The effect that compressed air massage (CAM) has on skeletal muscle has been ascertained by the morphological and morphometric evaluation of healthy vervet monkey and rabbit skeletal muscle. How CAM may influence the process of healing following a contusion injury is not known. To determine how CAM or other physiotherapeutic modalities may influence healing, it is necessary to create a minor injury that is both reproducible and quantifiable at the termination of a pre-determined healing period. An earlier study described changes in the morphology of skeletal muscle following a reproducible contusion injury. This study extended that work in that it attempted to quantify the 'severity' of such an injury. A 201 g, elongated oval-shaped weight was dropped seven times through a 1 m tube onto the left vastus lateralis muscle of four New Zealand white rabbits. Biopsies were obtained 6 days after injury from the left healing juxta-bone and sub-dermal muscle and uninjured (control) right vastus lateralis of each animal. The tissue was fixed in formal saline, embedded in wax, cut and stained with haematoxylin and phosphotungstic haematoxylin. The muscle was examined by light microscopy and quantification of the severity of injury made using a modified, 'in-house' morphological index and by the comparative morphometric measurement of the cross-sectioned epimysium and myofibres in injured and control muscle. The results showed that a single contusion causes multiple, quantifiable degrees of injury from skin to bone - observations of particular importance to others wishing to investigate contusion injury in human or animal models.

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Mobilisation of satellite cells following ischaemia and reperfusion in primate skeletal muscle

Cited by 8 publications

References 44 publications

Histological Study of the Effect of Platelet Rich Plasma on Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats

Histological Study of the Effect of Platelet Rich Plasma on Experimentally Induced Skeletal Muscles Injury in Adult Male Albino Rats

Critical Limb Ischaemia in Adult Human Skeletal Muscle Increases Satellite Cell Proliferation but Not Differentiation

The creation of a measurable contusion injury in skeletal muscle

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