Mobility of extracellular DNA within gonococcal colonies

Bender, Niklas; Hennes, Marc; Maier, Berenike

doi:10.1016/j.bioflm.2022.100078

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…In particular, the age and density of biofilm modulates the horizontal transfer of genes with more rapid spread observed in early biofilm and decreased dispersal in mature biofilms 17 . Biofilm is also considered to provide a potential eDNA reservoir as the mobility of DNA fragments through this matrix is hindered by increasing DNA length and presence of DUS, leading to accumulation of N. gonorrhoeae -specific eDNA 48 . In light of this, the potential of Lig E to modify the mobility of transformable eDNA by modulating biofilm properties, or potentially by acting on the transformation substrate itself by increasing its length or re-joining nicks resulting from nuclease or oxidative damage, is of considerable interest.…”

Section: Discussionmentioning

confidence: 99%

A role for the ATP-dependent DNA ligase Lig E ofNeisseria gonorrhoeaein biofilm formation

Pan,

Hicks,

Williamson

2023

Preprint

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The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisseria gonorrhoeae and characterised its growth and infection characteristics. This demonstrates that N. gonorrhoeae Lig E is a non-essential gene and its deletion does not cause defects in replication or survival of DNA-damaging stressors. Knock-out strains were partially defective in biofilm formation on an artificial surface as well as adhesion to epithelial cells which coupled with the predicted extracellular/ periplasmic location of Lig E indicates a role in extracellular DNA joining. In addition to in vivo characterisation, we have recombinantly expressed and assayed N. gonorrhoeae Lig E and determined the crystal structure of the enzyme-adenylate engaged with DNA substrate in an open non-catalytic conformation, providing insight into the binding dynamics of these minimal DNA ligases.

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Section: Discussionmentioning

confidence: 99%

A role for the ATP-dependent DNA ligase Lig E ofNeisseria gonorrhoeaein biofilm formation

Pan,

Hicks,

Williamson

2023

Preprint

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“…• Biofilms also provide ample opportunity for HGT, and its rates are typically higher in biofilms than in planktonic cultures 140,141 , although they may be affected by the spatial separation of donor and recipients 142 , the type of plasmid 143 , the sequence and length of the specific DNA fragment 144 , and the overall biofilm architecture (including presence of exopolysaccharides in the matrix) 145 .…”

mentioning

confidence: 99%

Biofilm antimicrobial susceptibility through an experimental evolutionary lens

Coenye

Bové

Bjarnsholt

2022

npj Biofilms Microbiomes

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Experimental evolution experiments in which bacterial populations are repeatedly exposed to an antimicrobial treatment, and examination of the genotype and phenotype of the resulting evolved bacteria, can help shed light on mechanisms behind reduced susceptibility. In this review we present an overview of why it is important to include biofilms in experimental evolution, which approaches are available to study experimental evolution in biofilms and what experimental evolution has taught us about tolerance and resistance in biofilms. Finally, we present an emerging consensus view on biofilm antimicrobial susceptibility supported by data obtained during experimental evolution studies.

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A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation

Pan,

Singh,

Hanning

et al. 2024

BMC Microbiol

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Background The ATP-dependent DNA ligase Lig E is present as an accessory DNA ligase in numerous proteobacterial genomes, including many disease-causing species. Here we have constructed a genomic Lig E knock-out in the obligate human pathogen Neisseria gonorrhoeae and characterised its growth and infection phenotype. Results This demonstrates that N. gonorrhoeae Lig E is a non-essential gene and its deletion does not cause defects in replication or survival of DNA-damaging stressors. Knock-out strains were partially defective in biofilm formation on an artificial surface as well as adhesion to epithelial cells. In addition to in vivo characterisation, we have recombinantly expressed and assayed N. gonorrhoeae Lig E and determined the crystal structure of the enzyme-adenylate engaged with DNA substrate in an open non-catalytic conformation. Conclusions These findings, coupled with the predicted extracellular/ periplasmic location of Lig E indicates a role in extracellular DNA joining as well as providing insight into the binding dynamics of these minimal DNA ligases.

show abstract

Mobility of extracellular DNA within gonococcal colonies

Cited by 4 publications

References 57 publications

A role for the ATP-dependent DNA ligase Lig E ofNeisseria gonorrhoeaein biofilm formation

A role for the ATP-dependent DNA ligase Lig E ofNeisseria gonorrhoeaein biofilm formation

Biofilm antimicrobial susceptibility through an experimental evolutionary lens

A role for the ATP-dependent DNA ligase lig E of Neisseria gonorrhoeae in biofilm formation

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