Mobilization of Cd from human serum albumin by small molecular weight thiols

Physiologically relevant plasma d , l -homocysteine concentrations mobilize Cd from human serum albumin

“…The flow rate was 1.0 mL/min and the void volume (v 0 ) and the inclusion volume (v i ) were determined as previously described [43]. Cd detection was accomplished by connecting the exit of the HPLC-column to the pneumatic nebulizer of a Buck Model 200A flame atomic absorption spectrometer (FAAS)(Buck Scientific, East Norwalk, CT, USA) which served as the Cd-specific detector at 228.8 nm using polypropylene tubing.…”

“…The FAAS was operated with an air/acetylene flame (oxidant pressure: 241 kPa, fuel pressure: 83 kPa). The void volume (v 0 ) was determined by the injection of blue dextrane and the inclusion volume (v i ) was determined by the injection of a Cd-EDTA complex as described previously [43].…”

“…Employing a SEC column in conjunction with a FAAS, the injection of a Cd-HSA (1:1 molar ratio) complex revealed that 1.0 mM of Cys, l-glutathione or N-acetylcysteine each effectively mobilized Cd from HSA to from Cd-SMW thiol complexes [43]. Investigations into the metabolism of Cd in animal experiments conducted by others have revealed that taurine (Fig.…”

“…We have previously applied a HPLC-based approach to probe the SMW-ligand mediated abstraction of Cd from its binding sites on HSA using PBS-buffer as the mobile phase [43]. Employing a SEC column in conjunction with a FAAS, the injection of a Cd-HSA (1:1 molar ratio) complex revealed that 1.0 mM of Cys, l-glutathione or N-acetylcysteine each effectively mobilized Cd from HSA to from Cd-SMW thiol complexes [43].…”

“…We therefore decided to employ the previously developed SEC-FAAS-approach to investigate the effect of these compounds on the stability of a Cd-HSA complex. Since our previous studies have shown that SMW sulfur compounds effectively mobilize Cd from its binding sites on HSA [43], we also investigated l-methionine (Fig. 1c), hCys (Fig.…”