Mobilization of electroosmotic flow markers in capillary zone electrophoresis

Martínková, Eva; Křížek, Tomáš; Kubíčková, Anna; Coufal, Pavel

doi:10.1002/elps.202000301

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…For measurement of the EOF velocity and mobility, the selection of a suitable electroneutral marker is important. As shown in a recent article [97], some of the commonly used EOF markers may interact with the BGE constituents and may move with nonzero electrophoretic mobility. This may result in the biased EOF mobility of the markers and effective mobility of the analytes.…”

Section: Suppression Of Peptide Sorption and Control Of Eofmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.

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Section: Suppression Of Peptide Sorption and Control Of Eofmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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“…For measurement of the EOF velocity and mobility, the selection of a suitable electroneutral marker is important. Martinkova et al [120] have shown that some of the commonly used EOF markers may interact with the BGE constituents and may move with nonzero electrophoretic mobility. This may result in the biased EOF mobility of the markers and effective mobility of the analytes.…”

Section: Suppression Of Peptide Adsorption and Regulation Of Eofmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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“…The EOF in capillary columns and microchannels is important for CE and electroosmotic pumping applications [7][8][9]. However, because of the difficulties of fabricating microchannels by FDM 3D printing, few works [10,11] were found in the literature studying EOF for the most common materials used as thermoplastic filaments.…”

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confidence: 99%

Electroosmotic flow in fused deposition modeling (FDM) 3D‐printed microchannels

et al. 2022

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Electroosmotic flow (EOF) was determined in tridimensional (3D)‐printed microchannels with dimensions smaller than 100 µm. Fused deposition modeling 3D printing using thermoplastic filaments of PETG (polyethylene terephthalate glycol), PLA (polylactic acid), and ABS (acrylonitrile butadiene styrene) were used to fabricate the microchannels. The current monitoring method and sodium phosphate solutions at different pH values (3–10) were used for the EOF mobility (µEOF) measurements, which ranged from 2.00 × 10−4 to 12.52 × 10−4 cm2 V−1 s−1. The highest and the smallest µEOF were obtained for the PLA and PETG microchannels, respectively. Adding the cationic surfactant cetyltrimethylammonium bromide to the sodium phosphate solution caused EOF direction reversion in all the studied microchannels. The obtained results can be interesting for developing 3D‐printed microfluidic devices, in which EOF is relevant.

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Mobilization of electroosmotic flow markers in capillary zone electrophoresis

Cited by 4 publications

References 42 publications

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Electroosmotic flow in fused deposition modeling (FDM) 3D‐printed microchannels

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