Mobilization of stem cells by granulocyte colony-stimulating factor for the regeneration of myocardial tissue after myocardial infarction

Kuethe, Friedhelm; Figulla, Hans R; Voth, M.; Richartz, Barbara M.; Opfermann, Thomas; Sayer, Herbert G.; Krack, Andreas; Fritzenwanger, Michael; Höffken, K.; Gottschild, D; Werner, Gabbi

doi:10.1055/s-2004-820062

Cited by 31 publications

(26 citation statements)

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“…Recently, however, bone marrow-derived cells (BMC) were suggested to serve as a valuable source for the regeneration of damaged tissues (5). Homing and engraftment of BMCs in damaged nonhematopoietic organs, such as vascular tissue (6), myocardium (7)(8)(9)(10)(11)(12)(13)(14)(15) brain (16)(17)(18), liver (19)(20)(21)(22), kidney (23)(24)(25), lung (26 -30), and skin (31), have been observed and were suggested to contribute to the wound-healing process. In some tissues like myocardium (9,(12)(13)(14)(15)32), kidney (24), and liver (19), even improved function has been observed.…”

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confidence: 99%

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Mobilization of Bone Marrow Stem Cells by Granulocyte Colony-Stimulating Factor Ameliorates Radiation-Induced Damage to Salivary Glands

Lombaert¹,

Wierenga²,

Kok³

et al. 2006

Clinical Cancer Research

141

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Purpose: One of the major reasons for failure of radiotherapeutic cancer treatment is the limitation in dose that can be applied to the tumor because of coirradiation of the normal healthy tissue. Late radiation-induced damage reduces the quality of life of the patient and may even be life threatening. Replacement of the radiation-sterilized stem cells with unirradiated autologous stem cells may restore the tissue function. Here, we assessed the potential of granulocyte colony-stimulating factor (G-CSF)–mobilized bone marrow–derived cells (BMC) to regenerate and functionally restore irradiated salivary glands used as a model for normal tissue damage. Experimental Design: Male-eGFP+ bone marrow chimeric female C57BL/6 mice were treated with G-CSF, 10 to 60 days after local salivary gland irradiation. Four months after irradiation, salivary gland morphology and flow rate were assessed. Results: G-CSF treatment induced homing of large number of labeled BMCs to the submandibular glands after irradiation. These animals showed significant increased gland weight, number of acinar cells, and salivary flow rates. Donor cells expressed surface markers specific for hematopoietic or endothelial/mesenchymal cells. However, salivary gland acinar cells neither express the G-CSF receptor nor contained the GFP/Y chromosome donor cell label. Conclusions: The results show that BMCs home to damaged salivary glands after mobilization and induce repair processes, which improve function and morphology. This process does not involve transdifferentiation of BMCs to salivary gland cells. Mobilization of BMCs could become a promising modality to ameliorate radiation-induced complications after radiotherapy.

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confidence: 99%

“…A clinically attractive approach is to use granulocyte colony-stimulating factor (G-CSF) to mobilize bone marrow cells to the circulation. It has been reported that G-CSF is associated with improved cardiac function and survival after myocardial infarction in mice (9,12,32,33).…”

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confidence: 99%

Mobilization of Bone Marrow Stem Cells by Granulocyte Colony-Stimulating Factor Ameliorates Radiation-Induced Damage to Salivary Glands

Lombaert¹,

Wierenga²,

Kok³

et al. 2006

Clinical Cancer Research

141

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“…It has been reported that autologous BMSC mobilization can also heal damaged tissues. [23][24][25][26] Above studies provides a proof that autologous BMSC mobilization can also restore renal tubular structure and improve renal function.…”

Section: Introductionmentioning

confidence: 94%

Effect of Stem Cell Factor and Granulocyte-Macrophage Colony-Stimulating Factor-Induced Bone Marrow Stem Cell Mobilization on Recovery from Acute Tubular Necrosis in Rats

Zhang

Bai

et al. 2012

Renal Failure

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Background: Acute tubular necrosis (ATN) is the most common reason for acute kidney injury (AKI), and there is still an absence of effective therapies. Objective: To assess the value of bone marrow cell mobilization by stem cell factor (SCF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) therapy in rats with gentamicin-induced ATN. Methods: ATN was induced in male Sprague-Dawley (SD) rats with five daily high-dose intraperitoneal injections of gentamicin. Subcutaneous injections of SCF and GM-CSF were administered simultaneously and these cytokines were observed on days 2, 5, 10, 17, 24, and 31. Peripheral blood and renal tissue CD34+ cell count, mortality rate, blood urea nitrogen (BUN), serum creatinine (SCr), creatinine clearance rate (CCr), and histopathologic lesion scores were determined. Twelve hours after bone marrow ablation (BMA) by lethal X-ray radiation, specific pathogen-free (SPF) ATN rats were given five daily injections of SCF and GM-CSF. BUN, SCr, and histopathologic lesion scores were evaluated on days 2, 5, and 10. Results: Peripheral blood CD34+ cell count increased significantly in ATN rats between 2 and 10 days after SCF and GM-CSF injection. Mortality was reduced from 34.7% in the ATN group to 18.6% in the ATN+CSF. In addition, cytokines administration significantly decreased SCr and BUN. Moreover, cytokines rapidly ameliorated tubular injury. There was no significant effect on ATN rats after BMA. Conclusions: This study demonstrated that SCF and GM-CSF effectively mobilized bone marrow cells in ATN rats, and cytokines administration partially prevented gentamicin-induced ATN. These results suggest that bone marrow stem cell (BMSC) mobilization may be an effective therapy for ATN.

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“…Several clinical studies using G-CSF in AMI patients suggest improvement in left ventricular function. 18,19 These studies as well as positive studies using adult stem cells 17 have led to tremendous enthusiasm and hype. While small trials are initially needed to establish safety and feasibility, many of the trials examining the use of stem cells for cardiovascular disease treatment have limitations that make interpretation of efficacy difficult.…”

Section: A Dose Of Realitymentioning

confidence: 99%

Attempts to Recruit Stem Cells for Repair of Acute Myocardial Infarction

Kloner¹

2006

JAMA

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Mobilization of stem cells by granulocyte colony-stimulating factor for the regeneration of myocardial tissue after myocardial infarction

Abstract: In patients with acute MI, treatment with G-CSF to mobilize BMC appears to be well tolerable under clinical conditions. Improved cardiac function and perfusion may be attributed to BMC-associated promotion of myocardial regeneration and neovascularization.

Cited by 31 publications

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Mobilization of Bone Marrow Stem Cells by Granulocyte Colony-Stimulating Factor Ameliorates Radiation-Induced Damage to Salivary Glands

Mobilization of Bone Marrow Stem Cells by Granulocyte Colony-Stimulating Factor Ameliorates Radiation-Induced Damage to Salivary Glands

Effect of Stem Cell Factor and Granulocyte-Macrophage Colony-Stimulating Factor-Induced Bone Marrow Stem Cell Mobilization on Recovery from Acute Tubular Necrosis in Rats

Attempts to Recruit Stem Cells for Repair of Acute Myocardial Infarction

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