Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Coetzee, J. N.

doi:10.1099/00221287-108-1-103

Cited by 17 publications

(8 citation statements)

References 26 publications

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“…It is perhaps worth noting that such origins differ from those of RP4 (Towner & Vivian, 19763). Alternatively, the results obtained are not inconsistent with multiple sites of origin as found by Coetzee (1978) for plasmid R772 in Proteus mirabilis.…”

Section: Discussionsupporting

confidence: 52%

Gene Transfer in Acinetobacter calcoaceticus: Fertility Variants of the Sex Factor pAV1

Hinchliffe¹,

Vivian²

1980

Microbiology

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The naturally occurring transmissible plasmid pAVl mediates chromosome transfer and can exhibit two distinct levels of transmissibility in Acinetobacter calcoaceticus strain EBF65/65. The two states of pAV1 have been arbitrarily designated pAVl a (low frequency variant) and pAVl b (high frequency variant). Both variants have the same incompatibility and host range properties and each mobilizes two non-transmissible resistance determinants for tetracycline and neomycin. Sex factor activity has been shown to be stable: however, pAVl b fertility variants can be derived from pAVla donors following conjugal transfer of pAV1 into new recipient strains of EBF65/65.

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Section: Discussionsupporting

confidence: 52%

Gene Transfer in Acinetobacter calcoaceticus: Fertility Variants of the Sex Factor pAV1

Hinchliffe¹,

Vivian²

1980

Microbiology

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“…IncP group plasmids R26, R527, R702, R751, R906, R839, R934, R938, and R1033 (30), pUZ8 (31), R91A (32), and R995 (isolated by R. W. Hedges) were supplied by G. A. Jacoby. R772 (33) was provided by T. V. Potts; and pJP4 (34), by J. M. Pemberton. pJP4.1 (pJP4::Tn3) was isolated in this laboratory.…”

Section: Methodsmentioning

confidence: 99%

Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.

Figurski¹,

Pohlman²,

Bechhofer³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

116

143

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Cloning of specific regions of RK2, a broad host range incompatibility group P plasmid, has revealed three genes: kilA, kIB, and kiC. Each of these genes can cause loss of viability of an Escherichia col host. This effect on the host is normally prevented by the functions ofthree additional RK2 genes: korA, korB, and korC. Each kor gene is specific for a particular kil gene. The kil and kor genes are located in four distinct regions of the RK2 genome. The three kil genes are not clustered and, with the possible exception of kilA, they are also well separated from their corresponding kor genes. We have found that the korA and korB determinants are not peculiar to RK2 but instead are highly conserved throughout the incompatibility group P plasmids.

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“…1), that, like Tn511-3, has no associated phenotype. They also imply that the maintenance of R772 in its clinical strain (Coetzee, 1978), and since then in laboratory strains, was due to aminoglycoside selection pressure. Indeed, the latter may be a strict requirement, since expression of aphAI imposes a cost burden on its bacterial host (Kim et al, 2006).…”

Section: The Aphai Region Of Tn511 Is Inherently Unstablementioning

confidence: 99%

“…The accessory genes that occur in the two regions are not necessarily located at the same site in the backbone, so that plasmids can be distinguished phylogenetically based on the position and type of insert that is present (Kamachi et al, 2006;Sota et al, 2007). Of relevance to this study are the IncPb plasmids pB10 (Schlüter et al, 2003), pJP4 (Trefault et al, 2004), R772 (Coetzee, 1978) and R906 (Terakado & Mitsuhashi, 1974). The first two of these have been fully sequenced, and molecular maps, supported by limited sequence data, are available for R772 (~61 kb) and R906 (~58 kb) , 1989Smith et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Embedded elements in the IncPβ plasmids R772 and R906 can be mobilized and can serve as a source of diverse and novel elements

Petrovski

Stanisich

2011

Microbiology

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IncP plasmids are important contributors to bacterial adaptation. Their phenotypic diversity is due largely to accessory regions located in one or two specific parts of the plasmid. The accessory regions are themselves diverse, as judged from sequenced plasmids mostly isolated from nonclinical sources. To further understand the diversity, evolutionary history and functional attributes of the accessory regions, we compared R906 and R772, focusing on the oriV-trfA accessory region. These IncPb plasmids were from porcine and clinical sources, respectively. We found that the accessory regions formed potentially mobile elements, Tn510 (from R906) and Tn511 (from R772), that differed internally but had identical borders. Both elements appeared to have evolved from a TnAO22-like mer transposon that had inserted into an ancestral IncPb plasmid and then accrued additional transposable elements and genes from various proteobacteria. Structural comparisons suggested that Tn510 (and a descendent in pB10), Tn511 and the mer element in pJP4 represent three lineages that evolved from the same widely dispersed IncPb carrier. Functional studies on Tn511 revealed that its mer module is inactive due to a merT mutation, and that its aphAI region is prone to deletion. More significantly, we showed that by providing a suitable transposase gene in trans, the defective Tn510 and Tn511 could transpose intact or in part, and could also generate new elements (stable cointegrates and novel transposons). The ingredients for assisted transposition events similar to those observed here occur in natural microcosms, providing non-self-mobile elements with avenues for dispersal to new replicons and for structural diversification. This work provides an experimental demonstration of how the complex embedded elements uncovered in IncP plasmids and in other plasmid families may have been generated.

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Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Cited by 17 publications

References 26 publications

Gene Transfer in Acinetobacter calcoaceticus: Fertility Variants of the Sex Factor pAV1

Gene Transfer in Acinetobacter calcoaceticus: Fertility Variants of the Sex Factor pAV1

Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.

Embedded elements in the IncPβ plasmids R772 and R906 can be mobilized and can serve as a source of diverse and novel elements

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