MOCH-1 Cells: An Oligodendrocyte Cell Line Generated Using a Transgenic Approach

Popko, Brian; Hayes, Carol; Li, Yan; Kelly, Douglas G.; Murayama, Shigeo; Suzuki, Kinuko

doi:10.1007/978-1-4615-2435-9_11

Cited by 2 publications

(1 citation statement)

References 33 publications

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“…I), which have been shown to express IFN-7 receptors (Torres et al, 1995). We have examined the effects of IFN-7 on oligodendrocytes using the MOCH-1 cell line, which was derived from an oligodendroglioma that developed in a transgenic line of mice and expresses a variety of features of oligodendrocytes (Hayes et al, 1992;Li et aL, 1995 Popko et al, 1994). Furthermore, these cells are immunoreactive against antibodies to galactocerebroside, a glycolipid marker of mature oligodendrocytes, and abundantly express myelin protein genes (Hayes et al, 1992).…”

Section: Cellular Sites Of Ifn-y Action In Immune-mediated Demyelinationmentioning

confidence: 99%

The effects of interferon-γ on the central nervous system

et al. 1997

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Interferon-gamma (IFN-gamma) is a pleotropic cytokine released by T-lymphocytes and natural killer cells. Normally, these cells do not traverse the blood-brain barrier at appreciable levels and, as such, IFN-gamma is generally undetectable within the central nervous system (CNS). Nevertheless, in response to CNS infections, as well as during certain disorders in which the CNS is affected, T-cell traffic across the blood-brain barrier increases considerably, thereby exposing neuronal and glial cells to the potent effects of IFN-gamma. A larger portion of this article is devoted to the substantial circumstantial and experimental evidence that suggests that IFN-gamma plays an important role in the pathogenesis of the demyelinating disorder multiple sclerosis (MS) and its animal model experimental allergic encephalomyelitis (EAE). Moreover, the biochemical and physiological effects of IFN-gamma are discussed in the context of the potential consequences of such activities on the developing and mature nervous systems.

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Section: Cellular Sites Of Ifn-y Action In Immune-mediated Demyelinationmentioning

confidence: 99%

The effects of interferon-γ on the central nervous system

et al. 1997

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Morphological and molecular response of the MOCH‐1 oligodendrocyte cell line to serum and interferon‐γ: Possible implications for demyelinating disorders

Atashi

Hayes

et al. 1995

J of Neuroscience Research

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The regional loss of oligodendrocytes is thought to be an important pathological event in a variety of demyelinating diseases of the central nervous system (CNS). Various components of serum, which are normally excluded from the CNS by the blood-brain barrier, have been implicated as mediators of demyelinating disorders. We have examined the effects of high concentrations of serum (10% fetal bovine serum, FBS), as well as the cytokine interferon-gamma (IFN-gamma), on an oligodendrocyte cell line, MOCH-1 cells. These cells changed from phase-bright, small round cells with multiple thin, branched processes in 1% FBS medium to flat, fibroblast-like cells with large cell bodies when cultured in 10% FBS medium or 1% FBS medium containing IFN-gamma. These morphological changes were accompanied by a large increase in expression of the astrocyte marker, glial fibrillary acidic protein (GFAP), as detected by Northern and Western blot analyses. In addition, Northern blot and fluorescence-activated cell sorting analyses revealed that IFN-gamma induced a very large increase in major histocompatibility complex (MHC) class I expression in MOCH-1 cells. MHC class II mRNA induction by IFN-gamma was also seen. In contrast, 10% FBS did not elevate either MHC class I or class II mRNA levels in MOCH-1 cells. The morphological and molecular effects of 10% FBS and IFN-gamma were reversible. We suggest that the response of MOCH-1 cells to high concentrations of serum and IFN-gamma may reflect an important in vivo response to oligodendrocytes to perturbations that occur in demyelinating disorders.

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MOCH-1 Cells: An Oligodendrocyte Cell Line Generated Using a Transgenic Approach

Cited by 2 publications

References 33 publications

The effects of interferon-γ on the central nervous system

The effects of interferon-γ on the central nervous system

Morphological and molecular response of the MOCH‐1 oligodendrocyte cell line to serum and interferon‐γ: Possible implications for demyelinating disorders

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