Mode of Action and Synergy of Ceftazidime and Baicalein against &lt;i&gt;Streptococcus pyogenes&lt;/i&gt;

Siriwong, Supatcharee; Pimchan, Thippawan; Naknarong, Wanatkamon; Eumkeb, Griangsak

doi:10.4314/tjpr.v14i4.12

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…28 Baicalein can enhance the effects of ampicillin and gentamicin against oral bacteria (fractional inhibitory concentration index, FICI < 0.375–0.5 and fractional bactericidal concentration index, FBCI < 0.5). 29 Baicalein also exhibits synergy with ceftazidime against Streptococcus pyogenes 30 and cefotaxime against K. pneumoniae . 31 Baicalein inhibits the activity of penicillinase to enhance the antibacterial effects of penicillins in a dose-dependent manner.…”

Section: Antibacterial Herbs and Their Componentsmentioning

confidence: 99%

Antimicrobial activities and mechanisms of extract and components of herbs in East Asia

Liang

Huang

2022

RSC Adv.

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Section: Antibacterial Herbs and Their Componentsmentioning

confidence: 99%

Antimicrobial activities and mechanisms of extract and components of herbs in East Asia

Liang

Huang

2022

RSC Adv.

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“…Currently, Fourier transform infrared (FTIR) microspectroscopy is widely used as a tool to monitor the changes in various biological samples such as bacteria, apoptotic and necrotic cell death, stem cell differentiation, and adipocyte cells [14][15][16][17]. FTIR technique is based on the absorption of infrared (IR) light of chemical bonds by vibrational transitions.…”

Section: Introductionmentioning

confidence: 99%

Intracellular ROS Scavenging and Anti‐Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN‐γ‐Activated RAW264.7 Macrophages

Dunkhunthod

Talabnin

Murphy

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Oroxylum indicum (L.) Kurz has been used as plant-based food and herbal medicine in many Asian countries. The aim of the present study was to examine the antioxidant and anti-inflammatory activities of O. indicum extract (O. indicum) in RAW264.7 cells activated by LPS plus IFN-γ. The phytochemical compounds in O. indicum were identified by GC-MS and LC-MS/MS. Five flavonoids (luteolin, apigenin, baicalein, oroxylin A, and quercetin) and 27 volatile compounds were found in O. indicum. O. indicum presented antioxidant activities, including reducing ability by FRAP assay and free radical scavenging activity by DPPH assay. Moreover, O. indicum also suppressed LPS plus IFN-γ-activated reactive oxygen species generation in RAW264.7 macrophages. It possessed the potent anti-inflammatory action through suppressing nitric oxide (NO) and IL-6 secretion, possibly due to its ability to scavenge intracellular ROS. The synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy results showed the alteration of signal intensity and integrated areas relating to lipid and protein of the activated RAW264.7 macrophages compared to unactivated cells. This is the first report of an application of the SR-FTIR technique to evaluate biomolecular changes in activated RAW264.7 cells. Our results indicate that O. indicum may be used as a potential source of nutraceutical for the development of health food supplement or a novel anti-inflammatory herbal medicine.

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“…Previous studies found that ceftazidime had synergistic activity with baicalein, luteolin or quercetin, which shared Chromone structure, against Streptococcus pyogenase [ 23 , 44 ]. In the same way, Eumkeb and co-workers reported that certain β-lactam drugs plus galangin, quercetin or baicalein, which also shared chromone structure, showed synergistic activity against penicillin-resistant S. aureus [ 9 ].…”

Section: Resultsmentioning

confidence: 99%

Synergism and the mechanism of action of the combination of α-mangostin isolated from Garcinia mangostana L. and oxacillin against an oxacillin-resistant Staphylococcus saprophyticus

et al. 2016

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BackgroundGlobally, staphylococci have developed resistance to many antibiotics. New approaches to chemotherapy are needed and one such approach could be to use plant derived actives with conventional antibiotics in a synergestic way. The purpose of this study was to isolate α-mangostin from the mangosteen (Garcinia mangostana L.; GML) and investigate antibacterial activity and mechanisms of action when used singly and when combined with oxacillin against oxacillin-resistant Staphylococcus saprophyticus (ORSS) strains. The isolated α-mangostin was confirmed by HPLC chromatogram and NMR spectroscopy. The minimum inhibitory concentration (MIC), checkerboard and killing curve were determined. The modes of action of these compounds were also investigated by enzyme assay, transmission electron microscopy (TEM), confocal microscopic images, and cytoplasmic membrane (CM) permeabilization studies.ResultsThe MICs of isolated α-mangostin and oxacillin against these strains were 8 and 128 μg/ml, respectively. Checkerboard assays showed the synergistic activity of isolated α-mangostin (2 μg/ml) plus oxacillin (16 μg/ml) at a fractional inhibitory concentration index (FICI) of 0.37. The kill curve assay confirmed that the viability of oxacillin-resistant Staphylococcus saprophyticus DMST 27055 (ORSS-27055) was dramatically reduced after exposure to isolated α-mangostin (2 μg/ml) plus oxacillin (16 μg/ml). Enzyme assays demonstrated that isolated α-mangostin had an inhibitory activity against β-lactamase in a dose-dependent manner. TEM results clearly showed that these ORSS-27055 cells treated with this combination caused peptidoglycan and cytoplasmic membrane damage, irregular cell shapes and average cell areas were significantly larger than the control. Clearly, confocal microscopic images confirmed that this combination caused considerable peptidoglycan damage and DNA leakage. In addition, the CM permeability of ORSS-27055 was also increased by this combination of actives.ConclusionsThese findings provide evidence that isolated α-mangostin alone has not only some activity but also shows the synergistic activity with oxacillin against ORSS-27055. The chromone and isoprenyl structures could play a significant role in its action. This synergistic activity may involve three mechanisms of action. Firstly, potential effects of cytoplasmic membrane disruption and increases permeability. Secondly, inhibit β-lactamase activity. Finally, also damage to the peptidoglycan structure. We proposes the potential to develop a novel adjunct phytopharmaceutical to oxacillin for the treatment of ORSS. Future studies require clinical trials to establish if the synergy reported can be translated to animals and humans.

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Mode of Action and Synergy of Ceftazidime and Baicalein against <i>Streptococcus pyogenes</i>

Cited by 7 publications

References 23 publications

Antimicrobial activities and mechanisms of extract and components of herbs in East Asia

Antimicrobial activities and mechanisms of extract and components of herbs in East Asia

Intracellular ROS Scavenging and Anti‐Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN‐γ‐Activated RAW264.7 Macrophages

Synergism and the mechanism of action of the combination of α-mangostin isolated from Garcinia mangostana L. and oxacillin against an oxacillin-resistant Staphylococcus saprophyticus

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