Mode of Action of Herbicidal Derivatives of Aminomethylenebisphosphonic Acid. Part II. Reversal of Herbicidal Action by Aromatic Amino Acids

Forlani, Giuseppe; Kafarski, Paweł; Lejczak, Barbara; Wieczorek, Piotr

doi:10.1007/pl00006989

Cited by 28 publications

(26 citation statements)

References 8 publications

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“…'Wisconsin') plants caused by glyphosate and N -pyridylaminomethylenebisphosphonic acids. Herbicidal effects were reversed by aromatic amino acids using both bacterial and plant models, a finding that strongly supports this hypothesis (Forlani et al, 1997).…”

Section: Introductionmentioning

confidence: 61%

Amino Acid Application Can Be an Alternative to Prevent Glyphosate Injury in Glyphosate-Resistant Soybeans

Zobiole

Oliveira

Constantin

et al. 2012

Journal of Plant Nutrition

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2 Glyphosate-resistant (GR) soybeans have continuously increased; however, this expansion significantly increased the use of glyphosate and therefore, in some cases, has resulted in injury symptoms observed in GR soybean, known as "yellow flashing". Previous reports of interference of glyphosate with nutrient availability and utilization by GR soybean may be linked to this injury symptom. Also, because glyphosate interferes with amino acid synthesis, supplementation with exogenous amino acids may help GR soybean recover from adverse effects of glyphosate. Therefore, an experiment was designed to evaluate different amino acid concentrations. Near-isogenic and GR soybean varieties were grown in the greenhouse in two soils with and without glyphosate at different rates and amino acids were foliarly applied with and without glyphosate. In general, the photosynthetic variables, nutrient contents, and shoot and root dry biomass parameters were affected by glyphosate, however, use of amino acid formulations suppressed harmful effects of glyphosate on these parameters.

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Section: Introductionmentioning

confidence: 61%

Amino Acid Application Can Be an Alternative to Prevent Glyphosate Injury in Glyphosate-Resistant Soybeans

Zobiole

Oliveira

Constantin

et al. 2012

Journal of Plant Nutrition

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“…Thus the occurrence of a target in plant aromatic metabolism was investigated. While in vitro activity of EPSPS was found to be unaffected (Forlani et al 1997), that of the plastidic and Mn ++ -stimulated isoform of the first enzyme in the shikimate pathway, 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase (DAHPS, EC 4.1.2.15), was markedly reduced. A kinetic analysis indicated that the most effective compound N-2-(5-chloro-pyridyl)aminomethylene bisphosphonic acid (Cl-pyr-AMBPA) inhibits enzyme activity competitively with respect to the substrate erythrose-4-phosphate (E4P), ruling out the possibility of an inhibition simply based upon its metal-chelating properties (Forlani et al 1996).…”

Section: Introductionmentioning

confidence: 98%

The herbicidally active compound N-2-(5-chloro-pyridyl) aminomethylene bisphosphonic acid acts by inhibiting both glutamine and aromatic amino acid biosynthesis

Forlani¹,

Lejczak²,

Kafarski³

2000

Functional Plant Biol.

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Abstract. The effect of the herbicidally active compound N-2-(5-chloro-pyridyl)aminomethylene bisphosphonic acid (Cl-pyr-AMBPA), previously found in vitro to inhibit the activity of the first enzyme in the shikimate pathway 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, was investigated in vivo on suspension cultured cells of Nicotiana plumbaginifolia Viviani. Amino acid pool measurement showed an actual reduction of tyrosine, tryptophan and phenylalanine level following the addition of the compound to the growth medium. However, an even stronger effect was noticed for other amino acids, mainly glutamine. When the activity of the enzymes involved in the glutamate cycle was measured in the presence of Cl-pyr-AMBPA, glutamate synthase was unaffected, while glutamine synthetase was significantly inhibited. Contrary to the herbicide phosphinothricin, the inhibitor bound reversibly to the enzyme. Kinetic analysis accounted for an inhibition of uncompetitive type with respect to ammonium, glutamate and ATP, with K i values of 113, 97 and 39 µM, respectively. Only the exogenous supply of a mixture of glutamine and aromatic amino acids relieved cell growth inhibition, suggesting that the phytotoxic properties of Cl-pyr-AMBPA are due to inhibition of key enzymes in both the corresponding pathways.

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“…Esses mesmos resultados foram confirmados por Santos et al (2005), os quais descreveram que a adição desses aminoácidos aromáticos ao meio de cultura com B. japonicum preveniu a inibição do crescimento pelo glyphosate, pelo fato de esse microrganismo possuir esta enzima sensível ao glyphosate (Moorman et al, 1992;De Maria et al, 2006). Forlani et al (1997) também relatam que a adição de aminoácidos aromáticos preveniu parcialmente a inibição do crescimento de raízes de pepino (Cucumis sativus) causada pelo glyphosate e pelo ácido N-pirimidilaminometilenobifosfônico (Forlani et al, 1997).…”

Section: Introductionunclassified

Uso de aminoácido exógeno na prevenção de injúrias causadas por glyphosate na soja RR

Zobiole¹,

Jr²,

Constantin³

et al. 2010

Planta daninha

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RESUMO -O aumento da área cultivada de soja resistente ao glyphosate (RR) no Brasil é resultado do benefício dessa tecnologia no manejo de plantas daninhas. No entanto, a expansão da área de soja RR aumentou significativamente o uso de glyphosate e consequentemente, em alguns casos, têm sido observados sintomas de injúrias na soja RR conhecidos como yellow flashing ou amarelecimento das folhas superiores. Nesse sentido, dois experimentos, em diferentes anos, foram conduzidos em delineamento inteiramente casualizado, com quatro repetições. O primeiro teve o objetivo de avaliar a influência do glyphosate na soja RR nas variáveis fotossintéticas, nos parâmetros de nodulação e na biomassa seca da parte aérea e raiz, realizando-se a comparação entre os tratamentos BRS 242 RR sem glyphosate, BRS 242 RR + glyphosate e a isolinha parental não-RR cv. Embrapa 58, submetidas a uma dose de glyphosate de 1.200 g e.a. ha, aplicada no estádio V4. O segundo experimento foi conduzido visando reavaliar as variáveis fotossintéticas, de produção de biomassa e nodulação afetadas na soja RR pelo glyphosate no primeiro experimento. Entretanto, no segundo experimento foi avaliada a utilização de diversas modalidades de aplicação de aminoácidos (a.a.), sendo os diferentes tratamentos (sem a.a.; tratamento de semente com a.a; tratamento de semente com a.a. + aplicação foliar de a.a.; sem tratamento de sementes com a.a; e com aplicação foliar de a.a) combinados com diferentes doses de glyphosate (1.200 e 2.400 g e.a. ha ), objetivando uma provável recuperação das plantas de soja com sua utilização exógena. Em ambos os experimentos, as variáveis fotossintéticas, os parâmetros de nodulação e biomassa seca da parte aérea e raiz foram afetados pela aplicação do glyphosate, porém o segundo experimento evidenciou, de modo geral, que o uso de aminoácidos via tratamento de sementes associado com aplicação foliar pode ser uma estratégia para prevenir os efeitos indesejáveis desse herbicida na cultura da soja RR.Palavras-chave: fotossíntese, biomassa, nodulação, herbicida, Rhizobium.ABSTRACT -Cultivation of glyphosate-resistant (GR) soybeans has increased in Brazil as a result of the application of this technology in weed management systems developed for this crop. However, the expansion of GR soybean production has significantly increased the use of glyphosate and, in some cases, resulted in injury symptoms observed in GR soybean, known as "yellow flashing" or yellowing of the upper leaves. Thus, two experiments were conducted in different years. The first experiment aimed to evaluate the influence of glyphosate on GR soybeans regarding the photosynthetic variables, nodule parameters, and shoot and root dry biomass by comparing cultivar BRS 242 GR without glyphosate and BRS 242 RR + glyphosate at 1.200 g ha -1 at V4 growth stage, to the near isogenic non-GR parental line cv. Embrapa 58. The second experiment aimed to reassess the same parameters in GR soybeans at the V4 stage treated with glyphosate, plus the application of various amino acids, to...

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Mode of Action of Herbicidal Derivatives of Aminomethylenebisphosphonic Acid. Part II. Reversal of Herbicidal Action by Aromatic Amino Acids

Cited by 28 publications

References 8 publications

Amino Acid Application Can Be an Alternative to Prevent Glyphosate Injury in Glyphosate-Resistant Soybeans

Amino Acid Application Can Be an Alternative to Prevent Glyphosate Injury in Glyphosate-Resistant Soybeans

The herbicidally active compound N-2-(5-chloro-pyridyl) aminomethylene bisphosphonic acid acts by inhibiting both glutamine and aromatic amino acid biosynthesis

Uso de aminoácido exógeno na prevenção de injúrias causadas por glyphosate na soja RR

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