Mode of action of the postharvest biocontrol yeast, Pichia guilliermondii. I. Characterization of attachment to Botrytis cinerea

Wisniewski, Michael; Biles, Charles L.; Droby, Samir; McLaughlin, R. J.; Wilson, Charles L.; Chalutz, E.

doi:10.1016/0885-5765(91)90033-e

Cited by 237 publications

(116 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Arras et al (1998) also reported attachment of P. guilliermondii onto P. italicum mycelium. Wisniewski et al (1991) reported the attachment of P. guilliermondii onto Botrytis cinerea mycelium. C. oleophila I-182 in Aspire ® by Ecogen, Langhorne, PA (Sugar and Spotts, 1999;El Ghaouth et al, 2000) and Cryptococcus albidus in Yield Plus ® (Ancor Yeast, Cape Town, South Africa), both with antagonistic activiy based on hyperparasitism, which have already been commercialized in the United States (El Ghaouth et al, 2000;Droby et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro

Coelho¹,

Celli

Ono

et al. 2007

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro

Coelho¹,

Celli

Ono

et al. 2007

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Antagonistic yeasts have been selected mainly for their capability to rapidly colonize and grow in surface wounds, and subsequently to out compete the pathogen for nutrients and space. Competition for nutrients and space is believed to be the major component of the mode of action of antagonistic yeasts [39,40]. Besides competing for nutrients and space, antagonistic yeasts parasitize major postharvest pathogens directly, through strong attachment to their hyphae.…”

Section: Resultsmentioning

confidence: 99%

“…Besides competing for nutrients and space, antagonistic yeasts parasitize major postharvest pathogens directly, through strong attachment to their hyphae. This leads to partial disruption of hyphal wall structures [40].…”

Section: Resultsmentioning

confidence: 99%

Different Approaches of Bio-control Agents for Controlling Root Rot Incidence of Some Vegetables under Greenhouse Conditions

Abdel-Kader¹,

El-Mougy²,

Aly³

et al. 2012

IJAF

View full text Add to dashboard Cite

Different approaches of some antagonistic fungal, bacterial and yeast agents applied as seed treatment or soil drench was evaluated against various soil-borne pathogens causing vegetables root rot disease under greenhouse conditions. The tested pathogenic fungi were Alternaria solani Fusarium solani, F. oxysporum, Rhizoctonia solani, Sclerotium rolfsii, Macrophomina phaseolina and Pythium sp., meanwhile the tested bio-agents were Trichoderma harzianum, T. Viride and Bacillus subtilis, Pseudomonas flourescens and Sacchromyces serivisae. Significant reduction in the disease incidence was observed in bio-agent treatments in comparison with untreated control. Root rot incidence, at pre-emergence stage, significant effect was observed in bio-agent treatments as seed soaking comparing with soil drench treatment. The treated seeds showed a protective effect for seeds germination against the invasion by soil-borne pathogenic fungi. Meanwhile, soil drenched with different bio-agents showed more efficacy for reducing root rot incidence at post-emergence growth stage of tested vegetables, Cucumber, Cantaloupe, Tomato and Pepper. The obtained results revealed that the antagonist T. harzianum showed significant superior effect to reduce diseases incidence followed by B. subtilis. Also, the antagonists T. viride and P. fluorescence occupied significantly the second degree for reducing root rot incidence. The treatment with S. serevisiae had the lowest effect on disease incidence, although it significantly lesser than check control treatment. The present study demonstrates that application of bio-agents as seed treatment and soil drench may be useful for controlling root rot disease in field.

show abstract

“…Entretanto, Zhang et al (2005) não obtiveram resultados satisfatórios no controle de Botrytis cinerea com o sobrenadante do cultivo de C. laurentii, embora atingisse o controle total ao utilizar uma suspensão de células íntegras, indicando atuação por competição de nutrientes. Wisniewsky et al (1991) atribuíram o sucesso da aplicação de leveduras à facilidade de integrar o uso ao ambiente e sistema de manejo da fruta. Usall et al (2001) obtiveram ótimos resultados utilizando C. sake (cepa CPA-1) no biocontrole de doenças pós-colheita causadas por P. expansum em maçãs, com redução na ocorrência de frutas deterioradas superior a 70%.…”

Section: Figuraunclassified

Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por Penicillium expansum

et al. 2011

View full text Add to dashboard Cite

As perdas consideráveis no armazenamento de maçãs decorrem principalmente de desordens microbiológicas, causadas por Penicillium expansum, que além de colonizar o fruto e causar dano à polpa, produz a patulina, micotoxina teratogênica e cancerígena. Entre as alternativas ao tradicional tratamento químico de doenças pós-colheita de frutos, enfoca-se o biocontrole por leveduras antagonistas, com ênfase em linhagens killer, em função da baixa possibilidade de resíduos tóxicos e com ampla inocuidade demonstrada nos processos fermentativos. O objetivo deste trabalho foi analisar o potencial antagônico de leveduras no controle de P. expansum, mediante antifungigrama em meio sólido e líquido. Do total de 44 leveduras isoladas (16 de frutas, 10 de silagem de milho e 18 de formigueiro de laboratório), 20 apresentaram antagonismo perante esporos de P. expansum em ágar Meio Para Levedura, sendo Debaryomyces hansenii C1 responsável por maior atividade associada à competição por nutrientes (zona de inibição de 31 mm) e D. hansenii C7 por antibiose/hiperparasitismo (15 mm). Entretanto, o ensaio realizado com o sobrenadante de cultivo reduziu o número de cepas ativas em cinco, sendo Pichia ohmeri 158 e Candida guilliermondii P3 as de maior atividade antagônica. No antifungigrama em meio líquido (caldo MPL) o sobrenadante do cultivo de C. guilliermondii (25ºC/72 horas) inibiu 58,15% da germinação dos esporos de P. expansum e P. ohmeri (25ºC/48 horas) inibiu o desenvolvimento de hifas em 66,17%. A atividade do meio extracelular baseado em antibiose sugeriu mecanismo associado ao caráter killer, uma vez que ambas as leveduras foram positivas perante as linhagens padrão S. cerevisiae NCYC 1006 e P. kluyveri CAY-15. A diferença entre os sobrenadantes obtidos do cultivo destas leveduras isoladamente e em interação com o fungo teste, visando estímulo adicional na produção de substância antifúngica, não foi significativa (P > 0,05). De acordo com os resultados obtidos, conclui-se que a aplicação de leveduras antagonistas constitui ferramenta promissora no controle biológico de P. expansum em pós-colheita de maçã. Palavras-chave: Penicillium expansum, leveduras antagonistas, biocontrole DOI: 10.5433/1679 Abstract Considerable losses during apple fruit storage occur due to microbiological diseases, mainly caused by Penicillium expansum, which in addition to fruit pulp deterioration produces patulin, a mycotoxin with carcinogenic and teratogenic activity. Biological control of post-harvest disease by antagonist yeasts

show abstract

Mode of action of the postharvest biocontrol yeast, Pichia guilliermondii. I. Characterization of attachment to Botrytis cinerea

Cited by 237 publications

References 16 publications

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro

Different Approaches of Bio-control Agents for Controlling Root Rot Incidence of Some Vegetables under Greenhouse Conditions

Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por Penicillium expansum

Contact Info

Product

Resources

About