Mode of AmyR Binding to the CGGN₈AGG Sequence in theAspergillus oryzaetaaG2Promoter

Comprehensive Analysis of the DNA-Binding Specificity of an <i>Aspergillus nidulans</i> Transcription Factor, AmyR, Using a Bead Display System

“…The researchers suggested that rather than binding directly to an 11-base CGGAAATTTAA, AmyR interacted with a 14-base CGGAAATTT-AAAGG sequence as a dimer. 21) In 2010, bead display was employed for AmyR-binding profile characterization. From two partially randomized libraries, Kojima et al obtained CGG triplets that were considered critical for effective AmyR-DNA interaction, and one other sequence that was found in the upstream region of AmyR-regulated gene amyB.…”

“…Hence, additional quantitative analysis is necessary for quality control of high-throughput screening approaches. Quantitative methods, including EMSA and surface plasmon resonance (SPR) have been used to investigate the binding affinity against AmyR, 13,16,21) but these methods are generally time and labor consuming, and thus incompatible with today's high-throughput screening platforms.…”

Inducer-Dependent Nuclear Localization of a Zn(II)2Cys6Transcriptional Activator, AmyR, inAspergillus nidulans

“…[25][26][27] AmyR binds to the direct repeat of CGG triplets separated by 8 bases in the various amylase promoter regions or the CGGN 8 AGG site of the Taka-amylase A promoter. 25,[28][29][30] In this study, subcellular localization studies and C-terminal truncation analysis of AmyR were carried out to obtain insight into the mechanisms of AmyR mediated induction. We found that nuclear accumulation of the GFP-AmyR fusion protein is triggered by the -amylase inducer isomaltose.…”

Molecular Analysis of AsamyR Gene Encoding Transcriptional Factor for Amylolytic Gene from Shoyu Koji Mold, Aspergillus sojae KBN1340

“…The DNA binding domain of AsAmyR shares a completely identical sequence with those of A. nidulans and A. oryzae (Fig. 1B), implying that AsAmyR recognizes and binds the same sequence, CGGN 8 (C/ A)GG, as these AmyRs (Petersen et al, 1999, Gomi et al, 2000, Tani et al, 2001a, Tani et al, 2001b, Itoh et al, 2004, Nakamura et al, 2006. Furthermore, the other four domains of AsAmyR showed significant sequence identity with the A. on the conserved domain structure between A. nidulans AmyR and Saccharomyces cerevisiae Mal63p, Makita et al presumed that the inducer-dependent nuclear localization of AmyR in A. nidulans is controlled by Hsp90/Hsp70-based chaperone machinery.…”

“…AmyR is a specific transcriptional activator with Zn(II) 2 Cys 6 binuclear cluster DNA-binding motif. AmyR binds the CGGN 8 AGG sequence at approximately -200 in the taaG2 gene promoter which is required for inducible expression by starch or maltose (Petersen et al, 1999, Gomi et al, 2000, Tani et al, 2001a, Itoh et al, 2004.…”