Mode of Inhibition of Glucose Oxidase by Metal Ions

Nakamura, Satoshi; Ogura, Yasuyuki

doi:10.1093/oxfordjournals.jbchem.a128915

Cited by 45 publications

(29 citation statements)

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“…In the same study, it was suggested that metal-induced enzyme inhibition could be due to the generation of radical oxygen species through a series of Fenton-type reactions, which can, in turn, denature the enzyme by causing conformational changes in its secondary and/or tertiary structure. On the other hand, it was also reported that the inhibition by metal ions may not be due to blocking of the essential SH group but to a direct interaction between the FAD moiety of the enzyme and metal ions [30]. However, further investigation is needed in order to elucidate this point.…”

Section: Repeatability Reproducibility and Stability Of The Biosensormentioning

confidence: 99%

Glucose oxidase inhibition in poly(neutral red) mediated enzyme biosensors for heavy metal determination

Ghica

Brett

2008

Microchim Acta

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A biosensor for the determination of heavy metal cations based on glucose oxidase enzymatic inhibition has been developed. The biosensor was assembled on carbon film electrode supports with glucose oxidase immobilised by cross-linking with glutaraldehyde on top of a film of poly(neutral red) as redox mediator, prepared by electropolymerisation. The biosensor was used to determine the metallic cations, cadmium, copper, lead and zinc in the presence of chosen amounts of glucose. The detection limits were found to be 1 μg L −1 for cadmium, 6 μg L −1 for copper, 3 μg L −1 for lead and 9 μg L −1 for zinc. Inhibition constants were determined by using the Dixon plot, and the type of inhibition induced by the metallic cations was evaluated from Cornish-Bowden plots plus Dixon plots, it being found that the inhibition is reversible and competitive for cadmium, mixed for copper and lead and uncompetitive for zinc. Copper-inhibited glucose oxidase to a greater extent followed by cadmium, lead and zinc. Regeneration of the glucose oxidase response was studied by using Ethylene diamine tetracetic acid metal-chelating agent and the nonionic surfactant Triton X-100. The suitability of the biosensor for determination in foodstuffs or beverages which contain trace concentrations of metals was investigated by performing recovery tests in commercial milk samples.

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Section: Repeatability Reproducibility and Stability Of The Biosensormentioning

confidence: 99%

Glucose oxidase inhibition in poly(neutral red) mediated enzyme biosensors for heavy metal determination

Ghica

Brett

2008

Microchim Acta

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“…4] has been isolated from microbial sources and studied by several workers (1)(2)(3)(4)(5). However, some of the results obtained are inconsistent ; for instance, various molecular features including subunit structure (4)(5)(6)(7)(8) and the role of the sulfhydryl groups of this enzyme (4,(9)(10)(11).…”

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confidence: 99%

Purification, Properties, and Molecular Features of Glucose Oxidase from Aspergillus niger

Tsuge

Natsuaki

Ohashi

1975

The Journal of Biochemistry

140

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“…The procedure was adapted from Zong et al for glucose sensing with Ag NPs [20]. Glucose (0–20 mM; 50 µL) and GOx (200 µL, 16.3314 mg·mL −1 , 15 000 U·g −1 , in pH 5.42, 50 mM NaOAc) were combined in a 96-well plate and incubated (~50 min, RT) to allow H 2 O 2 production without Ag NCs because Ag + is known to interfere with GOx activity [33]. Freshly prepared Ag NCs (50 µL) were added and mixed by pipetting.…”

Section: Methodsmentioning

confidence: 99%

Glucose Sensor Using Redox Active Oligonucleotide-Templated Silver Nanoclusters

2019

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Redox active, photoluminescent silver nanoclusters templated with oligonucleotides were developed for glucose sensing. The silver nanoclusters had a photoluminescent emission at 610 nm that reversibly changed to 530 nm upon oxidation. The reversible emission change was measured with photoluminescent spectroscopy and used to detect H2O2, which is a by-product of the reaction of glucose with glucose oxidase. The ratio of the un-oxidised emission peak (610 nm) and the oxidised analogue (530 nm) was used to measure glucose concentrations up to 20 mM, well within glucose levels found in blood. Also, the reversibility of this system enables the silver nanoclusters to be reused.

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Mode of Inhibition of Glucose Oxidase by Metal Ions

Cited by 45 publications

References 0 publications

Glucose oxidase inhibition in poly(neutral red) mediated enzyme biosensors for heavy metal determination

Glucose oxidase inhibition in poly(neutral red) mediated enzyme biosensors for heavy metal determination

Purification, Properties, and Molecular Features of Glucose Oxidase from Aspergillus niger

Glucose Sensor Using Redox Active Oligonucleotide-Templated Silver Nanoclusters

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