Mode of pollen spread in clonal seed orchard of Pinus koraiensis

Abstract

The patterns of effective pollen dispersal and the relationships between pollen dispersal and genetic composition in Pinus koraiensis are still unclear. Hence, we investigated the mode of pollen dispersal of P. koraiensis in a clonal seed orchard in Lushuihe Forestry Bureau, Jilin Province, using SSR molecular markers tech-nique and the method of maximum likelihood. A total of 13 pairs of nuclear microsatellites po-lymorphic primers were used in the paternity analysis. We analyzed 100 progenies and 150 paternities. A total of 56 alleles were detected in 13 loci with 3-6 alleles (4.3 alleles in average) in a single locus. The primers of SsrPt_ctg7170 and SsrPt_ctg5333 had the maximum (6) and the minimum (3) alleles, respectively. The averaged values of observed heterozygosity, expected heterozygosity and polymorphism information content of experimental groups were 0.648, 0.601 and 0.533, respectively. The mating dis-tance of P. koraiensis followed an approximately normal distribution. The most effective pollen for the female parent came from male parent trees 15-45 m away with an average mating dis-tance of 32.60 m and the longest of 67.88 m. The pollen source of offspring was not random but greatly affected by the wind direction during the pollination season of P. koraiensis. These re-sults have important implications for seed or-chard design to improve the genetic quality of seeds and seed production

Phenotypic Variability and Genetic Diversity in a Pinus koraiensis Clonal Trial in Northeastern China

“…However, the values determined in this study were higher compared to results reported by [ 76 , 77 ], which analyzed the mating system in a complete P. koraiensis population containing seeds, male and female trees using 14 SSR markers. Our results corroborated previous studies [ 78 , 79 ], which indicated higher genetic diversity in Chinese natural Korean pine populations compared to that of other populations in the Russian region.…”

Genetic Diversity and Population Structure of Natural Pinus koraiensis Populations

“…In addition, for P. koraiensis, the level of genetic diversity in this study was higher than that in previous reports employing other means of detection, such as allozymes (H e = 0.183) [2], RAPD molecular markers (H e = 0.169) [20], and ISSR molecular markers (H e = 0.601) [3]. These differences may be due to the different numbers and types of genetic detection technologies used in the studies or to the different populations and biased sample sizes.…”

“…et Zucc., also known as Korean pine, is an evergreen tree belonging to the Pinus genus and Pinaceae family [1]. It is mainly distributed in the mountainous area of Northeast China, as well as in North Korea, Japan, and Far Eastern region of Russia [1][2][3]. P. koraiensis plays a key ecological role as the most important component of natural broad-leaved Korean pine forests, and it is also famous for its economic value, such as the production of good quality timber and edible seeds [4,5].…”

Estimation of Genetic Parameters and Wood Yield Selection Index in a Clonal Trial of Korean Pine (Pinus koraiensis) in Northeastern China

“…The improvement of Korean pine dates back more than a half century [7]. To date, significant gains have been realized, and several studies reported some improvements in growth rate, and seed and wood quality [8][9][10][11]. Facing multiple needs and a permanent global change, multi-objective approaches have been suggested for the sustainable management of forest resources [12].…”