The hydrogen isotopic compositions of the leaf-wax n-alkanes
(δD) and n-alkanoic acids
(δD) are known to reflect ambient climatic
conditions (including precipitation δD values,
δD). However, the climatic conditions of exactly
which period (i.e. early or entire period of the leaf’s lifespan) these
biomarkers represent, i.e. the seasonality in δD and
δD records, is still evolving. The seasonality
studies on the δD and δD values,
done only in extra-tropical regions, mostly indicate the
δD values are biased towards the early growing season
whereas δD values are not biased towards any season.
To decipher the seasonality in the δD and
δD records from the tropics, we conducted a
long-duration experiment wherein deciduous and evergreen species were
grown using normal water (δD = −2‰) during the early stages of the
leaf’s growth and later using isotopically-labeled water (δD = 1000‰).
Our experiment revealed (i) in deciduous and evergreen species,
δD and δD values reflect
δD during the early stages of the leaf’s growth,
(ii) synchronous synthesis of n-alkanes and n-alkanoic
acids, and (iii) in deciduous species, minor incorporation of the
previous year’s photosynthates in the leaf wax pool of the current
year’s mature leaves. Our study suggests that the δD
and δD records in the tropics are biased towards the
climatic conditions prevailing during the early stages of the leaf’s
growth. This bias should be considered while comparing the
δD values generated from the leaf wax proxy
records and isotope-enabled atmospheric circulation models.