Model experiment for practical application of inseminated sperm method for production of interspecific hybrids (Clarias gariepinus × Heterobranchus longifilis)

Quyến, Nguyễn Ngọc; Alebachew, Getachew Worku; Kucska, Balázs; Kovács, Gyula; Halasi-Kovács, Béla; Ferincz, Árpád; Staszny, Ádám; Horváth, László; Urbányi, Béla; Müller, Tamás

doi:10.1016/j.aqrep.2022.101418

Aquaculture Reports

2022

DOI: 10.1016/j.aqrep.2022.101418

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Model experiment for practical application of inseminated sperm method for production of interspecific hybrids (Clarias gariepinus × Heterobranchus longifilis)

Nguyễn Ngọc Quyến¹,

Getachew Worku Alebachew²,

Balázs Kucska³

et al.

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2024

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Effect of cryoprotectant and concentration on the sperm quality of walking catfish, Clarias batrachus, post-cryopreservation

Handayani,

Maulida,

Rahayu

et al. 2024

cryo letters

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BACKGROUND: Walking catfish, Clarias batrachus is one of the native and most popular freshwater catfish species in Indonesia. However, cultivation faces challenges, particularly due to the scarcity of larvae resulting from underdeveloped breeding technologies. Cryopreservation is a method of storing sperm to maintain viability for a long period and support the breeding technology of the fish. Cryoprotectant, in this context, plays an important role in determining the success of sperm cryopreservation. OBJECTIVE: To determine the best type and concentration of cryoprotectant for cryopreservation of walking catfish sperm. MATERIALS AND METHODS: A total of five different types of cryoprotectants, namely DMSO, glycerol, ethyl glycol, ethanol, and methanol, were tested at four concentration levels namely 0%, 5%, 10%, 15%, and 20%, each with four replications. RESULTS: The type and concentration of cryoprotectant had a significant effect on sperm motility and viability (P<0.05). The best outcomes were obtained with 5% DMSO and ethyl glycol, 10% glycerol and methanol, as well as 15% ethanol. CONCLUSION: The highest motility and viability values were obtained with 5% DMSO, resulting in its recommendation for cryopreservation of walking catfish sperm.

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Effect of cryoprotectant and concentration on the sperm quality of walking catfish, Clarias batrachus, post-cryopreservation

Handayani,

Maulida,

Rahayu

et al. 2024

cryo letters

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show abstract

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Model experiment for practical application of inseminated sperm method for production of interspecific hybrids (Clarias gariepinus × Heterobranchus longifilis)

Cited by 1 publication

References 54 publications

Effect of cryoprotectant and concentration on the sperm quality of walking catfish, Clarias batrachus, post-cryopreservation

Effect of cryoprotectant and concentration on the sperm quality of walking catfish, Clarias batrachus, post-cryopreservation

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