Model of gentamicin-induced nephrotoxicity and its amelioration by calcium and thyroxine

Ernest, Sylvain

doi:10.1016/0306-9877(89)90061-3

Cited by 10 publications

(1 citation statement)

References 63 publications

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“…Carageorgiou and cols (2005) also observed an inhibition of brain Na + , K + -ATPase in hyperthyroidism by using a similar hyperthyroidism animal model and proposed that it may be due to the increase of intracellular calcium by L-T4 (Ernest, 1989;Carageorgiou et al, 2005). They also evaluated TAS (total antioxidant status) as an oxidative stress marker and observed that hyperthyroid animals seem to have a shorter lifespan and, at advanced age, present a myelin deficit and that this may be due to the damage produced by the oxidative stress generated by an excess of THs (Pasquini and Adamo, 1994).…”

Section: Discussionmentioning

confidence: 91%

Effects of Thyroid Hormones on Memory and on Na+, K+-ATPase Activity in Rat Brain

Reis-Lunardelli¹,

Castro²,

Bavaresco³

et al. 2007

CNR

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Thyroid hormones (THs), including triiodothyronine (T3) and tetraiodothyronine (T4), are recognized as key metabolic hormones of the body. THs are essential for normal maturation and function of the mammalian central nervous system (CNS) and its deficiency, during a critical period of development, profoundly affects cognitive function. Sodium-potassium adenosine 5'-triphosphatase (Na(+), K(+)-ATPase) is a crucial enzyme responsible for the active transport of sodium and potassium ions in the CNS necessary to maintain the ionic gradient for neuronal excitability. Studies suggest that Na(+), K(+)-ATPase might play a role on memory formation. Moreover, THs were proposed to stimulate Na(+), K(+)-ATPase activity in the heart of some species. In this work we investigated the effect of a chronic administration of L-thyroxine (L-T4) or propylthiouracil (PTU), an antithyroid drug, on some behavioral paradigms: inhibitory avoidance task, open field task, plus maze and Y-maze, and on the activity of Na(+), K(+)-ATPase in the rat parietal cortex and hippocampus. By using treatments which have shown to induce alterations in THs levels similar to those found in hyperthyroid and hypothyroid patients, we aimed to understand the effect of an altered hyperthyroid and hypothyroid state on learning and memory and on the activity of Na(+), K(+)-ATPase. Our results showed that a hyper and hypothyroid state can alter animal behavior and they also might indicate an effect of THs on learning and memory.

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Section: Discussionmentioning

confidence: 91%

Effects of Thyroid Hormones on Memory and on Na+, K+-ATPase Activity in Rat Brain

Reis-Lunardelli¹,

Castro²,

Bavaresco³

et al. 2007

CNR

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Inhibition of Gentamicin Uptake into Cultured Mouse Proximal Tubule Epithelial Cells by L‐Lysine

Kaunitz

Cummins

Mishler

et al. 1993

The Journal of Clinical Pharma

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Gentamicin uptake and toxicity was studied in a nontransformed cell line obtained from the S1 segment of the proximal tubule epithelium of a transgenic mouse. Cytotoxicity was assayed using the dye 3-(4,-5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Gentamicin uptake was assayed by a fluorescence polarization assay. No differences in toxicity were found among cells incubated for 4 hours in complete culture medium, enriched Kreb's buffer alone, or enriched Krebs' buffer with added 300 micrograms/mL gentamicin, 0.5 mmol/L L-lysine, or gentamicin plus L-lysine. Uptake of 300 micrograms/mL gentamicin was minimal at zero time and increased as a function of time. Uptake of gentamicin at 4 hours was positively correlated with medium gentamicin concentration. Addition of 0.5 mmol/L L-lysine inhibited uptake of 300 micrograms/mL gentamicin 38.9 +/- 10.2%. No other amino acid, including D-lysine or arginine, significantly changed gentamicin uptake. The authors conclude that gentamicin and L-lysine share a specific uptake mechanism located in the apical membrane of renal proximal tubule cells.

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Pfaller¹,

Willinger²,

Heyman³

et al. 1998

Clinical Nephrotoxins

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Model of gentamicin-induced nephrotoxicity and its amelioration by calcium and thyroxine

Cited by 10 publications

References 63 publications

Effects of Thyroid Hormones on Memory and on Na+, K+-ATPase Activity in Rat Brain

Effects of Thyroid Hormones on Memory and on Na+, K+-ATPase Activity in Rat Brain

Inhibition of Gentamicin Uptake into Cultured Mouse Proximal Tubule Epithelial Cells by L‐Lysine

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