Model system for multifunctional delivery nanoplatforms based on DNA-Polymer complexes containing silver nanoparticles and fluorescent dye

Kasyanenko, N. A.; Bakulev, V. M.; Perevyazko, Igor; Некрасова, Т. Н.; Назарова, О. В.; Slita, A. V.; Zolotova, Yu. I.; Panarin, E. F.

doi:10.1016/j.jbiotec.2016.08.010

Cited by 16 publications

(16 citation statements)

References 59 publications

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“…The luminescence of the DAPI dye (4′,6-diamidino-2-phenylindole) in DNA–AzoTAB solutions enabled us to identify the location of surfactant on the DNA helix. DAPI exhibits two types of binding to DNA [ 38 , 39 ] that are unambiguously distinguished with luminescence ( Figure 8 ) [ 40 ]. The intense luminescence of DAPI at low r < 0.1 (r is the ratio of DAPI to DNA phosphates molar concentrations) with a maximum at 460 nm and excitation at 340 nm ( Figure 8 a), indicates the strong A-T-specific DAPI binding to DNA in the minor groove.…”

Section: Resultsmentioning

confidence: 99%

DNA Interaction with Head-to-Tail Associates of Cationic Surfactants Prevents Formation of Compact Particles

et al. 2018

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Cationic azobenzene-containing surfactants are capable of condensing DNA in solution with formation of nanosized particles that can be employed in gene delivery. The ratio of surfactant/DNA concentration and solution ionic strength determines the result of DNA-surfactant interaction: Complexes with a micelle-like surfactant associates on DNA, which induces DNA shrinkage, DNA precipitation or DNA condensation with the emergence of nanosized particles. UV and fluorescence spectroscopy, low gradient viscometry and flow birefringence methods were employed to investigate DNA-surfactant and surfactant-surfactant interaction at different NaCl concentrations, [NaCl]. It was observed that [NaCl] (or the Debye screening radius) determines the surfactant-surfactant interaction in solutions without DNA. Monomers, micelles and non-micellar associates of azobenzene-containing surfactants with head-to-tail orientation of molecules were distinguished due to the features of their absorption spectra. The novel data enabled us to conclude that exactly the type of associates (together with the concentration of components) determines the result of DNA-surfactant interaction. Predomination of head-to-tail associates at 0.01 M < [NaCl] < 0.5 M induces DNA aggregation and in some cases DNA precipitation. High NaCl concentration (higher than 0.8 M) prevents electrostatic attraction of surfactants to DNA phosphates for complex formation. DAPI dye luminescence in solutions with DNA-surfactant complexes shows that surfactant tails overlap the DNA minor groove. The addition of di- and trivalent metal ions before and after the surfactant binding to DNA indicate that the bound surfactant molecules are located on DNA in islets.

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Section: Resultsmentioning

confidence: 99%

DNA Interaction with Head-to-Tail Associates of Cationic Surfactants Prevents Formation of Compact Particles

et al. 2018

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“…It is necessary to emphasize that DAPI binding with phosphates is observed only at a high DAPI concentration in DNA solution (the emission has a maximal intensity at z = 0.3 and λ ex = 420 nm). 22 Because of its much higher quantum yield, DAPI luminescence in the minor groove can also be observed at z = 0.3 at the excitation wavelength of 340 nm. In our experiment, we used λ ex = 380 nm because of the strong absorption of CoPc at 340 nm (see Figure 2).…”

Section: Resultsmentioning

confidence: 99%

DNA Complexes with Cobalt(II) Phthalocyanine Disodium Disulfonate

et al. 2019

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The interaction of cobalt phthalocyanine disodium disulfonate (CoPc) with calf thymus DNA in solutions was investigated by UV/vis spectrophotometry, circular dichroism (CD), and hydrodynamic methods (viscosity and flow birefringence). Two types of CoPc binding to DNA were observed. Fast CoPc interactions with DNA via external binding to phosphates were accompanied by the formation of stack-type phthalocyanine structures on the periphery of the DNA helix. The optical absorption spectra of such CoPc complexes with DNA were analyzed in order to obtain a binding constant K = (4.8 ± 0.4) × 104 M–1. CD spectra show the increasing optical activity of phthalocyanines bonded to DNA. DNA plays the role of a matrix, contributing to an increase in their stacking interactions. The CD spectrum of DNA varies slightly. The second type of cobalt-to-DNA binding manifests itself over a certain time. It can be associated with the reorganization of ligands in the cobalt coordination sphere by introducing DNA atoms. In our experiments, such binding was observed after storage of solutions for approximately 20 h at a temperature of 4 °C. It was shown that the minor groove of DNA remains free in CoPc–DNA complexes. CoPc does not bind with the most important group for metal coordinating to DNA in the major groove (N7 guanine). We completely excluded the intercalation binding model. The planes of phthalocyanines in CoPc–DNA complexes are oriented predominantly normal to the axis of the DNA helix. DNA rigidity (persistent length) does not change. This follows from the data on the measurement of the optical anisotropy and intrinsic viscosity of DNA in complexes.

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“…As we all know, Ag NPs released silver ions with high biological activity in the antibacterial process, as they penetrated the cell barrier and destroyed the organisms [22][23][24]28]. We speculated that the existence of dispersants hindered the release of silver ions from Ag NPs, thus inhibiting the antibacterial activity.…”

Section: Journal Of Nanomaterialsmentioning

confidence: 99%

“…In addition, special DNA sequences make it sensitive to HepG-2 cells [18]. Ag NPs have been developed as biosensors [19] and drug carriers [20] and have been applied in popular areas such as diagnosis and treatment of diseases [21,22], biological detection [23], and environmental analysis [24].…”

Section: Introductionmentioning

confidence: 99%

Effect of Properties of Silver Nanoparticles Coated with Polar Material and the Antibacterial Activity on Marine Pathogenic Bacteria

Feng¹,

Deng²,

Lai³

et al. 2020

Journal of Nanomaterials

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Silver nanoparticles (Ag NPs) have become one of the current research hotspots and are used in many fields such as electrochemistry, energy, bioanalysis, and environmental monitoring, especially in the field of antibacterial research. In this study, we investigated the effect of properties of Ag NPs coated with polar materials. Ag NPs covered by a dispersant that was triethylene glycol monoethyl ether was stable and conquered the aggregation of Ag NPs. The effect of the dispersant on biocompatibility was explored through interaction experiments between Ag NPs and DNA sequence. The coated Ag NPs could adsorb DNA, and the fluorescence of FAM-DNA could be quenched by Ag NPs. The adsorption and desorption experiments of DNA showed that the order of DNA functional groups on the interaction process was phosphate>T>C>A>G. Moreover, we selected marine pathogenic bacteria to test the antibacterial effect of Ag NPs coated with a polar dispersant. The polar material had a certain inhibitory effect on the antibacterial activity of Ag NPs. However, small molecules such as bases could interact on the surface Ag NPs and release Ag+ to perform the antibacterial activity. The results could contribute to the further application of Ag NPs.

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Model system for multifunctional delivery nanoplatforms based on DNA-Polymer complexes containing silver nanoparticles and fluorescent dye

Cited by 16 publications

References 59 publications

DNA Interaction with Head-to-Tail Associates of Cationic Surfactants Prevents Formation of Compact Particles

DNA Interaction with Head-to-Tail Associates of Cationic Surfactants Prevents Formation of Compact Particles

DNA Complexes with Cobalt(II) Phthalocyanine Disodium Disulfonate

Effect of Properties of Silver Nanoparticles Coated with Polar Material and the Antibacterial Activity on Marine Pathogenic Bacteria

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