Model systems for regeneration:<i>Arabidopsis</i>

Mathew, Mabel Maria; Prasad, Kalika

doi:10.1242/dev.195347

Cited by 25 publications

(13 citation statements)

References 114 publications

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“…The many advantages of Arabidopsis thaliana and the wide repertoire of regenerative responses in this species have provided some insight into the molecular mechanisms underlying tissue repair and organ regeneration [ 2 , 3 ]. Two broad categories of regeneration models have been established; hereafter, these models are referred to as tissue culture-induced regeneration and wound-induced regeneration [ 4 ]. Key transcription factor families and hormone crosstalk mechanisms involved in plant regeneration have been extensively studied in A. thaliana [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Metabolic Reprogramming during Wound-Induced Organ Formation in Tomato Hypocotyl Explants

Larriba

Sánchez-García

Martínez‐Andújar

et al. 2021

IJMS

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Plants have remarkable regenerative capacity, which allows them to survive tissue damage after exposure to biotic and abiotic stresses. Some of the key transcription factors and hormone crosstalk mechanisms involved in wound-induced organ regeneration have been extensively studied in the model plant Arabidopsis thaliana. However, little is known about the role of metabolism in wound-induced organ formation. Here, we performed detailed transcriptome analysis and used a targeted metabolomics approach to study de novo organ formation in tomato hypocotyl explants and found tissue-specific metabolic differences and divergent developmental pathways. Our results indicate that successful regeneration in the apical region of the hypocotyl depends on a specific metabolic switch involving the upregulation of photorespiratory pathway components and the differential regulation of photosynthesis-related gene expression and gluconeogenesis pathway activation. These findings provide a useful resource for further investigation of the molecular mechanisms involved in wound-induced organ formation in crop species such as tomato.

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Section: Introductionmentioning

confidence: 99%

Tissue-Specific Metabolic Reprogramming during Wound-Induced Organ Formation in Tomato Hypocotyl Explants

Larriba

Sánchez-García

Martínez‐Andújar

et al. 2021

IJMS

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“…In certain circumstances (e.g. wounding), differentiated plant cells can reprogram to become new stem cells, divide and redifferentiate for organ regeneration [57,58]. P. patens is an excellent system to investigate cell reprogramming and regeneration due to its fast and broadly occuring cell pluripotency [59].…”

Section: Regenerationmentioning

confidence: 99%

“…In angiosperms, regeneration is often reduced to localised stem cell pools (e.g. the base of leaves), takes longer to establish, and it is multicellular and asynchronous at the explant level [58,67].…”

Section: Regenerationmentioning

confidence: 99%

Mosses: Accessible Systems for Plant Development Studies

Floriach-Clark¹,

Tang²,

Willemsen³

2022

Model Organisms in Plant Genetics

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Mosses are a cosmopolitan group of land plants, sister to vascular plants, with a high potential for molecular and cell biological research. The species Physcomitrium patens has helped gaining better understanding of the biological processes of the plant cell, and it has become a central system to understand water-to-land plant transition through 2D-to-3D growth transition, regulation of asymmetric cell division, shoot apical cell establishment and maintenance, phyllotaxis and regeneration. P. patens was the first fully sequenced moss in 2008, with the latest annotated release in 2018. It has been shown that many gene functions and networks are conserved in mosses when compared to angiosperms. Importantly, this model organism has a simplified and accessible body structure that facilitates close tracking in time and space with the support of live cell imaging set-ups and multiple reporter lines. This has become possible thanks to its fully established molecular toolkit, with highly efficient PEG-assisted, CRISPR/Cas9 and RNAi transformation and silencing protocols, among others. Here we provide examples on how mosses exhibit advantages over vascular plants to study several processes and their future potential to answer some other outstanding questions in plant cell biology.

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“…Although the type and magnitude of regeneration between species varies greatly, the totipotent nature of plants make them amenable systems for studying this remarkable phenomenon (Ikeuchi et al, 2019). One way to classify the repertoire of plant regenerative responses is on the basis of mode of regeneration, namely mechanical injury-induced and tissue-cultureinduced regeneration (Mathew and Prasad, 2021). While mechanical injury-induced regeneration mostly involves the replacement of a lost cell, tissue, or organ (van den Berg et al, 1995;Durgaprasad et al, 2019;Efroni et al, 2016;Heyman et al, 2016;Radhakrishnan et al, 2020;Xu et al, 2006), tissueculture-induced regeneration mediates the rebuilding of an entire organism from scratch.…”

Section: Introductionmentioning

confidence: 99%