Model systems for the study of Enterococcal colonization and infection

Goh, Hwee Mian Sharon; Yong, Adeline Mei Hui; Chong, Kelvin Kian Long; Kline, Kimberly

doi:10.1080/21505594.2017.1279766

Cited by 102 publications

(84 citation statements)

References 254 publications

(402 reference statements)

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“…Supplementary Table S4 reports the modulation in expression of the genes characterized as related to virulence and antimicrobial resistance 4 , 34 on daptomycin exposure, based on this transcriptomic study. Among the 24 potential virulence genes described in E .…”

Section: Resultsmentioning

confidence: 99%

Small RNAs in vancomycin-resistant Enterococcus faecium involved in daptomycin response and resistance

Sinel

Augagneur

Sassi

et al. 2017

Sci Rep

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Vancomycin-resistant Enterococcus faecium is a leading cause of hospital-acquired infections and outbreaks. Regulatory RNAs (sRNAs) are major players in adaptive responses, including antibiotic resistance. They were extensively studied in gram-negative bacteria, but less information is available for gram-positive pathogens. No sRNAs are described in E. faecium. We sought to identify a set of sRNAs expressed in vancomycin-resistant E. faecium Aus0004 strain to assess their roles in daptomycin response and resistance. Genomic and transcriptomic analyses revealed a set of 61 sRNA candidates, including 10 that were further tested and validated by Northern and qPCR. RNA-seq was performed with and without subinhibitory concentrations (SICs) of daptomycin, an antibiotic used to treat enterococcal infections. After daptomycin SIC exposure, the expression of 260 coding and srna genes was altered, with 80 upregulated and 180 downregulated, including 51% involved in carbohydrate and transport metabolisms. Daptomycin SIC exposure significantly affected the expression of seven sRNAs, including one experimentally confirmed, sRNA_0160. We studied sRNA expression in isogenic mutants with increasing levels of daptomycin resistance and observed that expression of several sRNAs, including sRNA_0160, was modified in the stepwise mutants. This first genome-wide sRNA identification in E. faecium suggests that some sRNAs are linked to antibiotic stress response and resistance.

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Section: Resultsmentioning

confidence: 99%

Small RNAs in vancomycin-resistant Enterococcus faecium involved in daptomycin response and resistance

Sinel

Augagneur

Sassi

et al. 2017

Sci Rep

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“…First, Enterococcus faecalis and Pseudomonas aeruginosa were identified as two highly collagenolytic bacteria capable of causing leaks in both rats and mice. The discovery of E. faecalis as a leak pathogen is particularly important as it is the most common pathogen to be cultured from a leaking anastomosis in patients and the most difficult to eradicate from the intestinal tract. Although E. faecalis is classified as a low‐abundance commensal species among the normal microbiota, when properly provoked, it can bloom and behave as a virulent pathogen.…”

Section: Discussionmentioning

confidence: 99%

Low-fat/high-fibre diet prehabilitation improves anastomotic healing via the microbiome: an experimental model

Hyoju

Adriaansens

Wienholts

et al. 2020

Journal of British Surgery

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Background: Both obesity and the presence of collagenolytic bacterial strains (Enterococcus faecalis) can increase the risk of anastomotic leak. The aim of this study was to determine whether mice chronically fed a high-fat Western-type diet (WD) develop anastomotic leak in association with altered microbiota, and whether this can be mitigated by a short course of standard chow diet (SD; low fat/high fibre) before surgery.Methods: Male C57BL/6 mice were assigned to either SD or an obesogenic WD for 6 weeks followed by preoperative antibiotics and colonic anastomosis. Microbiota were analysed longitudinally after operation and correlated with healing using an established anastomotic healing score. In reiterative experiments, mice fed a WD for 6 weeks were exposed to a SD for 2, 4 and 6 days before colonic surgery, and anastomotic healing and colonic microbiota analysed.Results: Compared with SD-fed mice, WD-fed mice demonstrated an increased risk of anastomotic leak, with a bloom in the abundance of Enterococcus in lumen and expelled stool (65-90 per cent for WD versus 4-15 per cent for SD; P = 0⋅010 for lumen, P = 0⋅013 for stool). Microbiota of SD-fed mice, but not those fed WD, were restored to their preoperative composition after surgery. Anastomotic healing was significantly improved when WD-fed mice were exposed to a SD diet for 2 days before antibiotics and surgery (P < 0⋅001).

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“…Kan dolaşım enfeksiyon insidansı yoğun bakım hastalarında %1'den kemik iliği transplantı yapılmış hastalarda %36'ya kadar değişmektedir (4) . Gram pozitif bakteriler ile oluşan kan dolaşım enfeksiyonlarının %45'i enterokoklar ile ilişkilidir (5) .…”

Section: Introductionunclassified

In Vitro Daptomycin and Linezolid Susceptibility Profiles of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Blood Cultures

Öcal¹,

Gürbüz²,

Dansuk³

et al. 2017

TMCD

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Kan Kültürlerinden İzole Edilen Enterococcus faecium ve ABSTRACT In vitro Daptomycin and Linezolid Susceptibility Profiles of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Blood Cultures Objective: Enterococcal infections are among the leading causes of health care-associated infections, and in the treatment of these infections gradually increasing rate of antimicrobial resistance issue is encountered all over theworld. Resistance to glycopeptides and high-level aminoglycosides also increases the use of antibiotics such as linezolid and daptomycin against resistant gram-positive bacteria. Detection of in vitro efficacies of daptomycin and linezolid against Enterococcus faecium and Enterococcus faecalis strains isolated from blood cultures was aimed in this study Material and Methods

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Model systems for the study of Enterococcal colonization and infection

Cited by 102 publications

References 254 publications

Small RNAs in vancomycin-resistant Enterococcus faecium involved in daptomycin response and resistance

Small RNAs in vancomycin-resistant Enterococcus faecium involved in daptomycin response and resistance

Low-fat/high-fibre diet prehabilitation improves anastomotic healing via the microbiome: an experimental model

In Vitro Daptomycin and Linezolid Susceptibility Profiles of Enterococcus faecium and Enterococcus faecalis Strains Isolated from Blood Cultures

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