Modeling and experimental studies of enhanced cooling by medical gauze for cell cryopreservation by vitrification

Zhang, Yuntian; Zhao, Gang; Hossain, Safwan; He, Xiaoming

doi:10.1016/j.ijheatmasstransfer.2017.06.036

Cited by 21 publications

(24 citation statements)

References 27 publications

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“…The smooth thermal history indicate that there was no appreciable latent heat release during the cooling process, suggest that the CPA solution underwent glass transition during cooling. 62 Overall, the temperature in the sample with nano-warming was increased, probably due to the improved warming rate during the first few seconds under an AC magnetic field.…”

Section: Resultsmentioning

confidence: 97%

Dual Suppression Effect of Magnetic Induction Heating and Microencapsulation on Ice Crystallization Enables Low-Cryoprotectant Vitrification of Stem Cell–Alginate Hydrogel Constructs

Liu

Zhao

Chen

et al. 2018

ACS Appl. Mater. Interfaces

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Stem cells microencapsulated in hydrogel as stem cell-hydrogel constructs have wide applications in the burgeoning cell-based medicine. Due to their short shelf life at ambient temperature, long-term storage or banking of the constructs is essential to the "off-the-shelf" ready availability needed for their widespread applications. As a high-efficiency, easy-to-operate, low-toxicity, and low-cost method for long-term storage of the constructs, low-cryoprotectant (CPA) vitrification has attracted tremendous attention recently. However, we found many cells in the stem cell-alginate constructs (∼500 μm in diameter) could not attach to the substrate post low-CPA vitrification with ∼2 M penetrating CPAs. To address this problem, we introduced nanowarming via magnetic induction heating (MIH) of FeO nanoparticles to minimize recrystallization and devitrification during the warming step of the low-CPA vitrification procedure. Our results indicate that high-quality stem cell-alginate hydrogel constructs with an intact microstructure, high immediate cell survival (>80%), and greatly improved attachment efficiency (by nearly three times, 68% versus 24%) of the encapsulated cells could be obtained post-cryopreservation with nanowarming. Moreover, the cells encapsulated in the cell-hydrogel constructs post-cryopreservation maintained normal proliferation under 3D culture and retained intact biological function of multilineage differentiation. This novel low-CPA vitrification approach for cell cryopreservation enabled by the combined use of alginate hydrogel microencapsulation and FeO nanoparticles-mediated nanowarming may be valuable in facilitating the widespread application of stem cells in the clinic.

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Section: Resultsmentioning

confidence: 97%

Dual Suppression Effect of Magnetic Induction Heating and Microencapsulation on Ice Crystallization Enables Low-Cryoprotectant Vitrification of Stem Cell–Alginate Hydrogel Constructs

Liu

Zhao

Chen

et al. 2018

ACS Appl. Mater. Interfaces

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“…For the nylon mesh, the density was set to be 1.15 g/ml, temperature-dependent thermal conductivity and heat capacity obtained from National Institute of Standards and Technology (NIST) 35 – 37 . Convective heat flux was used as the boundary conditions with convective heat transfer coefficient set as 300 W/(m 2 *K) 38 , 39 . Warming rates at different cross-sections through the center point of embryos were compared for two extreme conditions.…”

Section: Methodsmentioning

confidence: 99%

Cryopreservation method for Drosophila melanogaster embryos

Zhan

Hays

et al. 2021

Nat Commun

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The development of a widely adopted cryopreservation method remains a major challenge in Drosophila research. Here we report a robust and easily implemented cryopreservation protocol of Drosophila melanogaster embryos. We present innovations for embryo permeabilization, cryoprotectant agent loading, and rewarming. We show that the protocol is broadly applicable, successfully implemented in 25 distinct strains from different sources. We demonstrate that for most strains, >50% embryos hatch and >25% of the resulting larvae develop into adults after cryopreservation. We determine that survival can be significantly improved by outcrossing to mitigate the effect of genetic background for strains with low survival after cryopreservation. We show that flies retain normal sex ratio, fertility, and original mutation after successive cryopreservation of 5 generations and 6-month storage in liquid nitrogen. Lastly, we find that non-specialists are able to use this protocol to obtain consistent results, demonstrating potential for wide adoption.

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“…Forced convective heat transfer coefficient of 500 W m K −1 was used. [ 47 ]…”

Section: Methodsmentioning

confidence: 99%

Conduction Cooling and Plasmonic Heating Dramatically Increase Droplet Vitrification Volumes for Cell Cryopreservation

et al. 2021

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Droplet vitrification has emerged as a promising ice‐free cryopreservation approach to provide a supply chain for off‐the‐shelf cell products in cell therapy and regenerative medicine applications. Translation of this approach requires the use of low concentration (i.e., low toxicity) permeable cryoprotectant agents (CPA) and high post cryopreservation viability (>90%), thereby demanding fast cooling and warming rates. Unfortunately, with traditional approaches using convective heat transfer, the droplet volumes that can be successfully vitrified and rewarmed are impractically small (i.e., 180 picoliter) for <2.5 m permeable CPA. Herein, a novel approach to achieve 90–95% viability in micro‐liter size droplets with 2 m permeable CPA, is presented. Droplets with plasmonic gold nanorods (GNRs) are printed onto a cryogenic copper substrate for improved cooling rates via conduction, while plasmonic laser heating yields >400‐fold improvement in warming rates over traditional convective approach. High viability cryopreservation is then demonstrated in a model cell line (human dermal fibroblasts) and an important regenerative medicine cell line (human umbilical cord blood stem cells). This approach opens a new paradigm for cryopreservation and rewarming of dramatically larger volume droplets at lower CPA concentration for cell therapy and other regenerative medicine applications.

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Modeling and experimental studies of enhanced cooling by medical gauze for cell cryopreservation by vitrification

Cited by 21 publications

References 27 publications

Dual Suppression Effect of Magnetic Induction Heating and Microencapsulation on Ice Crystallization Enables Low-Cryoprotectant Vitrification of Stem Cell–Alginate Hydrogel Constructs

Dual Suppression Effect of Magnetic Induction Heating and Microencapsulation on Ice Crystallization Enables Low-Cryoprotectant Vitrification of Stem Cell–Alginate Hydrogel Constructs

Cryopreservation method for Drosophila melanogaster embryos

Conduction Cooling and Plasmonic Heating Dramatically Increase Droplet Vitrification Volumes for Cell Cryopreservation

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