Modeling and optimization of antibacterial activity of the chitosan‐based hydrogel films using central composite design

Abstract

In the present study, hydrogel films composed of chitosan-poly(vinyl alcohol)-gelatin-thyme honey were successfully prepared by casting method, and their anti-bacterial properties were modeled and optimized. Antibacterial properties of the prepared films were analyzed by applying agar diffusion method. Staphylococcus aureus and Pseudomonas aeruginosa were tested as Gram-positive and Gram-negative bacteria, respectively. In order to obtain the composition of the film with maximum inhibition zone against both above-mentioned bacterial strains, the experiments were designed using response surface methodology based on five-level central composite design with four parameters, including concentrations of chitosan, poly(vinyl alcohol), gelatin, and honey. The results indicated that the prepared samples had good antibacterial activities against these two studied bacteria strains. Response surface method is conducted to develop mathematical models for process responses. Variance analysis on the experimental data shows that inhibition zone can be predicted effectively with quadratic models. In addition, swelling properties and rate of water vapor transmission of the prepared hydrogel films were studied. Due to the successful results, this hydrogel film has an excellent potential to be explored further as a wound healing material. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2544-2553, 2016.

Rheological and Mechanical Analyses of Felbinac Cataplasms by Using Box–Behnken Design

“…The ANOVA results for Y1 and Y2 provided F values of 50.65 and 18.78, as compared to the critical values from the cutoff points for the F distribution (=0.05), i.e., 3.9 and 4.1, respectively. These F values suggest that the derived quadratic models had significant influences on the responses [ 8 ]. R 2 and the adjusted R 2 values for Y1 were 98.92% and 96.96%, and for Y2 were 91.25% and 86.40%, respectively, which demonstrate the accuracy of the tests and the fitness of the results with the proposed models [ 9 ].…”

A functional chitosan-based hydrogel as a wound dressing and drug delivery system in the treatment of wound healing

“…Chitosan-PVA hydrogels have been widely used as wound dressing, and a series of studies have shown these composite materials enhanced wound healing as well as antibacterial activities. 53,[113][114][115][116][117] Related research also proved that chitosan-PVA hydrogel exhibited a good bactericidal activity against E. coli. The hydrogel with greater chitosan concentration (60% and 80%), had a better cell viability, proliferation, and blood clotting ability.…”

“…114 If honey or bee venom was added into the chitosan-PVA hydrogel, it strengthened the anti-inammatory effect and antibacterial activity, hence, enhanced wound healing. [115][116][117] All of these researches indicated chitosan-PVA hydrogels have excellent potential as wound dressings.…”

Development of an Intelligent Photosensitive Antibacterial Wound Dressing: Simultaneous Detection and Treatment of Bacterial Infection for Accelerated Wound Healing

“…However, given that Ag ions have been proven to be highly toxic, wound healing is often delayed. Thus, modified chitosan or chitosan derivatives and new alginate‐based antibacterial hydrogels dressings have attracted increased research attention …”

“…Thus, modified chitosan or chitosan derivatives and new alginate-based antibacterial hydrogels dressings have attracted increased research attention. [9][10][11][12][13] Despite the advancements and the wide range of dressings available, wound management remains extremely challenging because of the susceptibility of a wound to bacterial infection. [14] Especially, diagnosis of bacterial infection is not straightforward and speed of detection is essential in clinical conditions.…”