Modeling and simulation of fructooligosaccharides synthesis in a batch basket reactor

Detofol, M. R.; Aguiar‐Oliveira, Elizama; Bustamante-Vargas, Cindy Elena; Soares, Alexandre Batista de Jesus; Soares, Mónica Beatriz Alvarado; Maugeri, Francisco

doi:10.1016/j.jbiotec.2015.06.410

Cited by 13 publications

(8 citation statements)

References 25 publications

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“…The yield obtained in this work (0.65 g FOS /g initial Sucrose ) was considerably higher than the maximum yield usually found for FOS production with purified (Nishizawa et al, 2001) and crude (Sangeetha et al, 2005) microorganism enzymes, in a two-step fermentation (0.55-0.60 g FOS /g initial Sucrose ). For example, in a work conducted in a batch basket reactor using an adsorbed and partially purified β-fructofuranosidase from Rhodotorula sp., a FOS yield of 0.60 g FOS /g initial Sucrose was obtained after 96 h fermentation (Detofol et al, 2015). The highest yield found in the present study represents an improvement that may have high impact at industrial level, since the lower yield of the two-step fermentation is the main drawback of the process.…”

Section: Fos Production In Bioreactormentioning

confidence: 49%

Process development for the production of prebiotic fructo-oligosaccharides by penicillium citreonigrum

Nobre

Nascimento

Silva

et al. 2019

Bioresource Technology

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A new isolated P. citreonigrum URM was selected to produce fructooligosaccharides (FOS) in an efficient, economical and fast one-step fermentation. Optimal culture conditions were stablished by experimental design. Experiments run in bioreactor resulted in a high yield, content, productivity and purity of FOS (0.65 ± 0.06 g FOS /g initial Sucrose , 126.3 ± 0.1 g/L, 2.28 ± 0.08 g/L.h and 61 ± 0%). The FOS mixture was purified up to 92% (w/w) with an activated charcoal column. FOS produced were able to promote lactobacilli and bifidobacteria growth. Higher bacteria cell density was obtained for microbial-FOS mixtures than commercial Raftilose ® P95. Some strains grew even faster in the FOS mixture produced than in all other carbon sources. FOS were resistant to the simulated gastrointestinal conditions. A high amount of a reducing trisaccharide was identified in the FOS produced mixture, possibly neokestose, which may explain the great prebiotic potential of the FOS.

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Section: Fos Production In Bioreactormentioning

confidence: 49%

Process development for the production of prebiotic fructo-oligosaccharides by penicillium citreonigrum

Nobre

Nascimento

Silva

et al. 2019

Bioresource Technology

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“…The yield of sucrose conversion into FOS is highly species‐dependent, and, in general, the enzymes produced by fungi belonging to the genus Aspergillus are responsible for the highest industrial yields, around 60% . This genus is deeply explored to produce industrial enzymes due to the ability of its members to grow on solid or liquid substrates and to largely produce extracellular enzymes.…”

Section: Introductionmentioning

confidence: 99%

“…The yield of sucrose conversion into FOS is highly species-dependent, and, in general, the enzymes produced by fungi belonging to the genus Aspergillus are responsible for the highest industrial yields, around 60%. 8 This genus is deeply explored to produce industrial enzymes due to the ability of its members to grow on solid or liquid substrates and to largely produce extracellular enzymes. Moreover, some Aspergillus strains are generally recognized as safe (GRAS) microorganisms, according to the Food and Drug Administration, and used for human and animal nutrition or production of various enzymes used in the food industry.…”

mentioning

confidence: 99%

Biochemical characterization and kinetic/thermodynamic study of Aspergillus tamarii URM4634 β‐fructofuranosidase with transfructosylating activity

Oliveira

Silva

Converti

et al. 2019

Biotechnology Progress

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This study reports on the biochemical characterization as well as the kinetic and thermodynamic study of Aspergillus tamarii URM4634 β‐fructofuranosidase (FFase) with transfructosylating activity. Conditions for FFase activity were optimized by means of a central composite rotational design using pH and temperature as the independent variables, while residual activity tests carried out in the temperature range of 45–65°C enabled us to investigate FFase thermostability and estimate the kinetic and thermodynamic parameters of enzyme denaturation. Optimal conditions for sucrose hydrolysis and fructosyl transfer catalyzed by crude FFase were 50°C, and pH 6.0 and 7.4, respectively. The thermodynamic properties of irreversible enzyme inactivation were found to be activation energy of 293.1 kJ mol−1, and activation enthalpy, entropy, and Gibbs free energy in the ranges 290.3–290.4 kJ mol−1, 568.7–571.0 J mol−1 K−1, and 97.9–108.8 kJ mol−1, respectively. The results obtained in this study point out satisfactory enzyme activity and thermostability at temperatures commonly used for industrial fructo‐oligosaccharide (FOS) synthesis; therefore, this novel FFase appears to be a promising biocatalyst with great potential for long‐term FOS synthesis and invert sugar production. To the best of our knowledge, this is the first report on kinetic and thermodynamic parameters of an A. tamarii FFase.

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“…Response surface methodology (RSM) provides information about optimum levels of each variables, interaction between them and their effects to generate product with higherrate (Dilipkumar, Rajamohan, & Rajsimman, 2013;Mutanda et al, 2008;Rao & Satyanarayana, 2003). A packed-bed reactor can continuously generate products of enzyme action in controlled environment (Detofol et al, 2015;Ganaie et al, 2014;Jung et al, 2011;Nakamura et al, 1995). The present study describes production and characterization of exoinulinase from Aspergillus niger and use of inulin rich extract obtained from dandelion root as an alternative material for continuous generation of fructose syrup using packed-bed reactor.…”

Section: Introductionmentioning

confidence: 99%

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

Rawat

Soni

Kango

et al. 2017

Biocatalysis and Agricultural Biotechnology

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Modeling and simulation of fructooligosaccharides synthesis in a batch basket reactor

Cited by 13 publications

References 25 publications

Process development for the production of prebiotic fructo-oligosaccharides by penicillium citreonigrum

Process development for the production of prebiotic fructo-oligosaccharides by penicillium citreonigrum

Biochemical characterization and kinetic/thermodynamic study of Aspergillus tamarii URM4634 β‐fructofuranosidase with transfructosylating activity

Continuous generation of fructose from Taraxacum officinale tap root extract and inulin by immobilized inulinase in a packed-bed reactor

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