Modeling drug-induced mitochondrial toxicity with human primary cardiomyocytes

Tang, Xiaoli; Liu, Hong; Rao, Rongjia; Huang, Yafei; Dong, Mengqi; Xu, Miaomiao; Feng, Shanshan; Shi, Xun; Wang, Li; Wang, Zengwu; Zhou, Bingying

doi:10.1007/s11427-023-2369-3

Cited by 3 publications

(1 citation statement)

References 112 publications

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“…Adult human primary cardiomyocytes have increasingly become an important in vitro model in cardiovascular research due to their outstanding prediction of proarrhythmic risks, mitochondrial toxicity prediction, and delineation of disease mechanisms [ [3] , [4] , [5] ]. We have previously reported on the use of a tissue slicing-assisted method for the efficient isolation of human primary cardiomyocytes (hPCMs), a protocol that involves the use of mechanical agitation of the digestion solution to facilitate tissue dissociation [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Methylcellulose improves dissociation quality of adult human primary cardiomyocytes

Shi,

Rao,

et al. 2024

Heliyon

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Section: Introductionmentioning

confidence: 99%

Methylcellulose improves dissociation quality of adult human primary cardiomyocytes

Shi,

Rao,

et al. 2024

Heliyon

View full text Add to dashboard Cite

Cardioprotective strategies in myocardial ischemia-reperfusion injury: Implications for improving clinical translation

Tong,

Zhou

2024

Journal of Molecular and Cellular Cardiology Plus

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Effect and mechanism of T lymphocytes on human induced pluripotent stem cell-derived cardiomyocytes via Proteomics

Ye,

Xu,

Liu

et al. 2024

Stem Cell Res Ther

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Background Abnormalities in T cell activation play an important role in the pathogenesis of myocarditis, and persistent T cell responses can lead to autoimmunity and chronic cardiac inflammation, as well as even dilated cardiomyopathy. Although previous work has examined the role of T cells in myocarditis in animal models, the specific mechanism for human cardiomyocytes has not been investigated. Methods In this study, we constructed the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and established the T cell-mediated cardiac injury model by co-culturing with activated CD4 + T or CD8 + T cells that were isolated from peripheral mononuclear blood to elucidate the pathogenesis of myocardial cell injury caused by inflammation. Results By combination of quantitative proteomics with tissue and cell immunofluorescence examination, we established a proteome profile of inflammatory myocardia from hiPSC-CMs with obvious cardiomyocyte injury and increased levels of lactate dehydrogenase content, creatine kinase isoenzyme MB and cardiac troponin. A series of molecular dysfunctions of hiPSC-CMs was observed and indicated that CD4 + cells could produce direct cardiomyocyte injury by activating the NOD-like receptor signals pathway. Conclusions The data presented in our study established a proteome map of inflammatory myocardial based on hiPSC-CMs injury model. These results can provide guidance in the discovery of improved clinical treatments for myocarditis.

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Modeling drug-induced mitochondrial toxicity with human primary cardiomyocytes

Cited by 3 publications

References 112 publications

Methylcellulose improves dissociation quality of adult human primary cardiomyocytes

Methylcellulose improves dissociation quality of adult human primary cardiomyocytes

Cardioprotective strategies in myocardial ischemia-reperfusion injury: Implications for improving clinical translation

Effect and mechanism of T lymphocytes on human induced pluripotent stem cell-derived cardiomyocytes via Proteomics

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