Modeling growth, lipid accumulation and lipid turnover in submerged batch cultures of Umbelopsis isabellina

Meeuwse, Petra; Akbari, Payman; Tramper, J.; Rinzema, A.

doi:10.1007/s00449-011-0632-x

Cited by 32 publications

(30 citation statements)

References 25 publications

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“…), while also in several cases the process of the previously accumulated lipid degradation was considered (Meeuwse et al . ). Moreover, transcriptomic, proteomic and metabolic network state‐of‐the‐art studies have also been realized in micro‐organisms enabling the de novo lipid production process in which, dual substrates (i.e.…”

Section: Physiology Of Lipid Accumulation Processmentioning

confidence: 97%

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Lipids from yeasts and fungi: physiology, production and analytical considerations

et al. 2018

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The last years there has been a significant rise in the number of publications in the international literature that deal with the production of lipids by microbial sources (the 'single cell oils; SCOs' that are produced by the so-called 'oleaginous' micro-organisms). In the first part of the present review article, a general overview of the oleaginous micro-organisms (mostly yeasts, algae and fungi) and their potential upon the production of SCOs is presented. Thereafter, physiological and kinetic events related with the production of, mostly, yeast and fungal lipids when sugars and related substrates like polysaccharides, glycerol, etc. (the de novo lipid accumulation process) or hydrophobic substrates like oils and fats (the ex novo lipid accumulation process) were employed as microbial carbon sources, are presented and critically discussed. Considerations related with the degradation of storage lipid that had been previously accumulated inside the cells, are also presented. The interplay of the synthesis of yeast and fungal lipids with other intracellular (i.e. endopolysaccharides) or extracellular (i.e. citric acid) secondary metabolites synthesized is also presented. Finally, aspects related with the lipid extraction and lipidome analysis of the oleaginous micro-organisms are presented and critically discussed.

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Section: Physiology Of Lipid Accumulation Processmentioning

confidence: 97%

“…, ; Meeuwse et al . , , ). In several cases, the production of other storage materials than the microbial lipids (i.e.…”

Section: Physiology Of Lipid Accumulation Processmentioning

confidence: 99%

Lipids from yeasts and fungi: physiology, production and analytical considerations

et al. 2018

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“…Figure shows a simplified scheme of a lipid‐accumulating (oleaginous) organism . The organism uses an N‐source and a C‐source to produce cell mass, while C‐source alone can also be used for lipid production, carbohydrate production and maintenance.…”

Section: The Evaluated Processesmentioning

confidence: 99%

“…In this paper, we estimate the energy input needed for large‐scale production of lipids for biodiesel from organic waste material using lipid‐accumulating yeast and fungi. Lipid yields in several cultivation systems are calculated with recently developed kinetic models for the filamentous fungus Umbelopsis isabellina ; two of these models can also describe data from yeasts, which shows their general applicability . Using basic engineering principles, we estimate the energy use during cultivation and part of the substrate pre‐treatment and downstream processing steps.…”

Section: Introductionmentioning

confidence: 99%

Lipids from yeasts and fungi: Tomorrow's source of biodiesel?

Meeuwse

Sanders

Tramper

et al. 2013

Biofuels Bioprod Bioref

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In the search for new transport fuels from renewable resources, biodiesel from microbial lipids comes into view. We have evaluated the lipid yield and energy use of a process for production of biodiesel from agricultural waste using lipid‐accumulating yeast and fungi. We included different bioreactors for submerged and solid‐state fermentation in our evaluation. Using existing kinetic models, we predict lipid yields on substrate between 5% and 19% (w/w), depending on the culture system. According to the same models, improvement of the yield to 25–30% (w/w) is possible, for example by genetic modification of the micro‐organisms. The net energy ratio of the non‐optimized systems varies between 0.8 and 2.5 MJ produced per MJ used; energy use for pre‐treatment and for oxygen transfer are most important. For the optimized systems, the net energy ratio increases to 2.9–5.5 MJ produced per MJ used, which can compete very well with other biofuels such as bioethanol or algal biodiesel. This shows that, although quite some work still has to be done, microbial lipids have the potential to be tomorrow's source of biodiesel. © 2013 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Nonetheless, other models have been applied for different yeasts, molds or fungi [5], but only [11], [12] modeled the coupled production of citric acid and lipids growing on glucose and a mixture of glucose/stearin respectively. These models involved linear kinetics for citric acid production; Williams, Monod, Haldane, and Verhlust kinetics to describe growth; and Monod, linear, and substrate inhibition kinetic types for describing lipid accumulation.…”

Section: Introductionmentioning

confidence: 99%

Dynamical metabolic modelling for lipid accumulation by Yarrowia lipolytica growing on glucose

Robles-Rodriguez

Guillouet

Bideaux

et al. 2015

2015 23rd Mediterranean Conference on Control and Automation (MED)

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The oleaginous yeast Yarrowia lipolytica has been extensively studied due to its capacity to accumulate great amounts of lipids triggered by the excess of the carbon source and the limitation of nitrogen. However, under these conditions this yeast can also produce citric acid, which decreases lipid conversion yield. Few dynamical models are only available to describe lipid metabolism based on mass balances and different kinetic configurations. Nevertheless, cybernetic modeling has allowed extending optimization through the inclusion of internal regulation to identify the controlling steps and metabolic fluxes. In the present work a dynamic metabolic model was developed and applied with two variations, and was compared with experimental data carried out in fed-batch cultures of yeast Y. lipolytica on glucose as carbon source. Two independent data sets regarding nitrogen limitation and deficiency were used for parameter estimation. Results show a good fit of parameters on describing the dynamics of lipids and citric acid production.

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Modeling growth, lipid accumulation and lipid turnover in submerged batch cultures of Umbelopsis isabellina

Cited by 32 publications

References 25 publications

Lipids from yeasts and fungi: physiology, production and analytical considerations

Lipids from yeasts and fungi: physiology, production and analytical considerations

Lipids from yeasts and fungi: Tomorrow's source of biodiesel?

Dynamical metabolic modelling for lipid accumulation by Yarrowia lipolytica growing on glucose

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