The ignition timing of an SI engine is a critical parameter. The influence on residual gas, effective release energy, and emissions characteristics of ignition timing for the V-twin engine is investigated in this research. For this purpose, an experiment system was built with a dynamometer, and a model of the simulation was created. In this research, the ignition timing was varied from 10 to 45 degrees BTDC under full load operating conditions, with engine speeds ranging from 3000 to 10,000 rpm. Based on the output data, ignition timing has a major impact on the proportion of residual gas, efficient release energy, performance of the engine, and the emission characteristics. The smallest proportion of residual gas was 0.07% at 8000 rpm and ignition timing of 10 °CA. At 15 °CA of ignition timing, the highest efficient release energy was 0.817 kJ at 4000 rpm, while at 8000 rpm and 25 °CA of ignition timing, it was 0.8305 kJ. At 6000 rpm, the greatest braking torque of the engine was 21.57 Nm, while the minimal BSFC was 343.821 g/kWh. The nitrogen oxide emission and HC emission increase with the advanced ignition timing, but CO emission decreases.