Modeling of biomass production by Spirulina platensis as function of phosphate concentrations and pH regimes

Çelekli, Abuzer; Yavuzatmaca, Mehmet; Bozkurt, Hüseyin

doi:10.1016/j.biortech.2009.02.055

Cited by 69 publications

(46 citation statements)

References 25 publications

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“…Because nitrogen appears in the chlorophyll molecule, there is a relationship between nitrogen addition and chlorophyll contents (Rangel-Yagui et al 2004). Phosphorus affects the amount of chl a produced by S. platensis, and the optimum phosphate concentration was determined as 0.5 g/l (Costa et al 2003;Ç elekli et al 2009). According to our results, chl a content in group II was lower than the control.…”

Section: Discussionmentioning

confidence: 99%

Effect of an organic fertilizer on growth of blue-green alga Spirulina platensis

2011

Aquacult Int

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In this study, the influence of an agricultural liquid organic fertilizer on growth and biomass composition of Spirulina platensis Geitler was studied. In the first trial, five concentrations of organic fertilizer were arranged, e.g., 0.1, 0.25, 0.5, 1 and 2 ml/l, in which no other extra nutrients except bicarbonate were used. Maximum dry weight, chlorophyll a and specific growth rate were achieved in control group. In the second trial, initial fertilizer levels of the three groups were 0.1 ml/l and all groups contained sodium bicarbonate. One of the groups had 2.5 g/l NaNO 3 , while the other was supported with 0.1 ml/l fertilizer by two-day intervals to see the effect of nitrogen. In order to understand the effects of phosphate, 0.4 g K 2 HPO 4 was added to the other group as well as 2.5 g NaNO 3 . Between the experimental groups, the highest chlorophyll a value was found in control group as 53.97 mg/l. The chlorophyll value at group II (37.65 mg/l) was lower than the control group. The maximum dry weight and crude protein recorded in control group were 1,330 mg/l and 60.17%, respectively, when compared with 1,256 mg/l and 59.92% in group II. No significant differences were observed in crude protein values of Spirulina grown in control and group II (P \ 0.05). Consequently, it was seen that commercial liquid organic fertilizers may be replaced by Zarrouk's medium except for nitrate, phosphate and bicarbonate in S. platensis cultures in order to decrease the production costs.

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Section: Discussionmentioning

confidence: 99%

Effect of an organic fertilizer on growth of blue-green alga Spirulina platensis

2011

Aquacult Int

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“…Well-documented nutritional and therapeutic properties of A. platensis , mainly as a tool against malnutrition (3), have elicited a growing market of several thousand tons yearly. As an extremophile photosynthetic microorganism, A. platensis is suitable for mass-production (4), including for the mass-production of highly valued biopolymers (5). The high pH (up to 11.5) of its fully mineral culture medium provides an efficient barrier against other bacterial contamination (6).…”

Section: Genome Announcementmentioning

confidence: 99%

Whole-Genome Shotgun Sequence of Arthrospira platensis Strain Paraca, a Cultivated and Edible Cyanobacterium

et al. 2014

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“…Inoculums was done at the exponential phase of Spirulina in Zarrouk's medium which inoculated to 50 mL Zarrouk's medium in 100 mL glass Erlenmeyer up to standard 1 McFarland. Spirulina was grown on an illuminated Shaker Incubator, at 32°C, with a light intensity of 8200 Lux and a light period of 12:12 (15,16,(20)(21)(22).…”

Section: Spirulina Culturementioning

confidence: 99%

“…A selectable system is based on sensitivity of organism to an antibiotic and a resistant gene which contribute the resistant to this antibiotic. This research was aimed to find out the suitable selection resistant gene for Spirulina screening (14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Spirulina platensis Resistance to Different Antibiotics to Find a Selectable Marker for Genetic Transformation

Moazez

Memari

Ofoghi

et al. 2013

Jundishapur J Microbiol

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Background: Spirulina is one of the most profitable known microalgae in the world, which is used as food and superfood. In the other hand, Spirulina is a useful source of healthy components. It seems that the Spirulina is transformable so that the introduction of a selectable marker is needed. Objectives: The purpose of this study was to determine a suitable selection marker for Spirulina platensis. Thus, this experiment was designed to survey the response of Spirulina to different concentrations of candidate antibiotics including kanamycin, hygromycin, phosphinothricin, chloramphenicol and streptomycin in standard Zarrouk medium. Materials and Methods: S. platensis culture matched to 1 McFarland standard and its resistance to different antibiotics was studied by serial dilution of kanamycin, hygromycin, phosphinothricin, chloramphenicol and streptomycin. Biomass was detected after 7days using spectrophotometer and related growth graphs were illustrated. Results: Fresh culture of S. platensis reached to exponential phase on day 4 of experiment. While this phase was presented on day 5 for cultures containing kanamycin or hygromycin. The Spirulina cultured in medium supplied with basta, did not reach to growth phase however, chloramphenicol can stop the growth of Spirulina cells. Spirulina showed resistance to streptomycin with the concentration of 40 mg/L and higher. Therefore, streptomycin can use as selectable marker to detect GM (Genetic Modified) Spirulina spp. Conclusions:The streptomycin can be used as suitable selection marker in comparison with other introduced markers. For using this selectable marker, integration of a streptomycin resistant gene is needed. The gene aadA is a potential candidate. The aadA gene can be expressed under the control of upstream and downstream elements of S. platensis.

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Modeling of biomass production by Spirulina platensis as function of phosphate concentrations and pH regimes

Cited by 69 publications

References 25 publications

Effect of an organic fertilizer on growth of blue-green alga Spirulina platensis

Effect of an organic fertilizer on growth of blue-green alga Spirulina platensis

Whole-Genome Shotgun Sequence of Arthrospira platensis Strain Paraca, a Cultivated and Edible Cyanobacterium

Evaluation of Spirulina platensis Resistance to Different Antibiotics to Find a Selectable Marker for Genetic Transformation

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