Modeling of Flow Rate, Pore Size, and Porosity for the Dispensing-Based Tissue Scaffolds Fabrication

Li, M. G.; Tian, Xiaoyu; Chen, Daniel

doi:10.1115/1.3123331

Cited by 47 publications

(40 citation statements)

References 13 publications

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“…The structure of the scaffolds for the model developmentis shown in Figure 2, in which the strand diameter (D) and the horizontal span(Y) can be controlled during the scaffold fabrication [16,19]. Also shown in Figure2 are the distance between two adjacent horizontal (h xy ) and vertical (h z ) strands, which together represent the pore size.…”

Section: Scaffold Used For Model Developmentmentioning

confidence: 99%

“…While both the vertical pore size (h z ) and the horizontal distance (h xy ) are associated with the strand diameter (D) and the horizontal span (Y), respectively, the vertical pore size (h z ) is also affected by the scaffold material properties due to the fusion of the two strands. Based on the previous research in our group [16], the vertical pore size (h z ) is determined by the diameter of the strand (D), the density of the scaffold material (ρ), the elastic limit stress (τ e ), the horizontal span (Y) and the angle between the two layers (θ) (Figure 2). The approximate relationship can be described as follows:…”

Section: Scaffold Used For Model Developmentmentioning

confidence: 99%

“…In the present study, a chitosan solution with 40% hyroxylapatite (HA) gel is assumed to be used for the scaffold fabrication and its elastic limit stress (τ e ) is 11.0 Pa as identified in [16]. In the present study, the strand diameter D was varied from 0.2 to 0.4 mm, while the horizontal span Y was varied from 0.5 to 0.9 mm.The corresponding vertical pore sizes are given in Table 1.…”

Section: Scaffold Used For Model Developmentmentioning

confidence: 99%

“…In these studies, the scaffolds with irregular internal structures were significantly simplified in the model development, thus causing to the errors in the following simulation. Currently,scaffolds manufactured by rapid prototyping (RP) techniques have shown promising in various tissue engineering applications due to their controllable microstructure [15,16] For such scaffolds, the structure is regular and the geometry can be readily modelled by CAD methods. Singh et al [17,18] utilized commercial CFD software to create models of such scaffolds in bioreactorsand studied the influence of mechanical stimuli on the velocity and shear stress distribution.…”

Section: Introductionmentioning

confidence: 99%

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Modeling of the Flow within Scaffolds in Perfusion Bioreactors

Yan¹,

Chen²,

Bergstrom³

2012

AJBE

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Tissue engineering aims to produce artificial organs and tissues for transplant treatments, in which cultivating cells on scaffolds in bioreactors is of critical importance. To control the cultivating process, the knowledge of the fluid flow inside and around a scaffold in the bioreactor is essential. However, due to the complicated microstructure of a scaffold, it is difficult, or even impossible, to gain such knowledge experimentally. In contrast, numerical methods employing computational fluid dynamics (CFD) have proven promising to alleviate the problem. In this research the fluid flow in perfusion bioreactors is studied with numerical methods. The emphasis is on investigating the effect of the controllable parameters in both the scaffold fabrication (i.e., the diameter of scaffold strand and the distance between two strands) and cell culture process (i.e., the flow rate) on the distribution of shear stress within the scaffold in a perfusion bioreactor. The knowledge obtained in this study will allow for improved control strategies in scaffold fabrication and cell culturing experiments.

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Section: Scaffold Used For Model Developmentmentioning

confidence: 99%

Section: Scaffold Used For Model Developmentmentioning

confidence: 99%

Section: Scaffold Used For Model Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modeling of the Flow within Scaffolds in Perfusion Bioreactors

Yan¹,

Chen²,

Bergstrom³

2012

AJBE

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“…Fabricating a scaffold with the desired pore size and porosity is of great importance in tissue engineering (Li et al 2009). For bacterial cellulose scaffold, the definition of a specific pore size in a bacterial cellulose fibrous hydrogel is not relevant because the nanofibrils can be pushed aside by migrating cells (Bäckdahl et al 2006).…”

Section: Introductionmentioning

confidence: 99%

The influence of fermentation conditions and post-treatment methods on porosity of bacterial cellulose membrane

Tang

Jia

et al. 2009

World J Microbiol Biotechnol

151

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Bacterial cellulose has been found to be attractive as a novel scaffold material due to its unique material properties. Porosity is the most important morphological parameter in the design of scaffolds for tissue engineering. The effects of fermentation conditions (cultivation time and inoculation volume) and post-treatment methods (alkali treatment and drying methods) on the porosities of bacterial cellulose membranes were investigated. With extended cultivation time and increased inoculation volume, more micro-fibrils were secreted by bacteria, which resulted in a more compact structure and diminished porosity. The porosities of alkali-treated bacterial cellulose membranes was in the order of K 2 CO 3 [ Na 2 CO 3 [ KOH [ NaOH. Freeze-dried membranes had much higher porosity (92%) than the hot air-dried ones (65%). The experimental results suggested that bacterial cellulose with controlled porosities could be prepared by varying fermentation conditions and post-treatment methods. The resulting bacterial cellulose is regarded as a scaffold material of great potentialities.

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Design and Printing Strategies in 3D Bioprinting of Cell‐Hydrogels: A Review

Lee

Yeong

2016

Adv Healthcare Materials

275

189

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Bioprinting is an emerging technology that allows the assembling of both living and non‐living biological materials into an ideal complex layout for further tissue maturation. Bioprinting aims to produce engineered tissue or organ in a mechanized, organized, and optimized manner. Various biomaterials and techniques have been utilized to bioprint biological constructs in different shapes, sizes and resolutions. There is a need to systematically discuss and analyze the reported strategies employed to fabricate these constructs. We identified and discussed important design factors in bioprinting, namely shape and resolution, material heterogeneity, and cellular‐material remodeling dynamism. Each design factors are represented by the corresponding process capabilities and printing parameters. The process‐design map will inspire future biomaterials research in these aspects. Design considerations such as data processing, bio‐ink formulation and process selection are discussed. Various printing and crosslinking strategies, with relevant applications, are also systematically reviewed. We categorized them into 5 general bioprinting strategies, including direct bioprinting, in‐process crosslinking, post‐process crosslinking, indirect bioprinting and hybrid bioprinting. The opportunities and outlook in 3D bioprinting are highlighted. This review article will serve as a framework to advance computer‐aided design in bioprinting technologies.

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Modeling of Flow Rate, Pore Size, and Porosity for the Dispensing-Based Tissue Scaffolds Fabrication

Cited by 47 publications

References 13 publications

Modeling of the Flow within Scaffolds in Perfusion Bioreactors

Modeling of the Flow within Scaffolds in Perfusion Bioreactors

The influence of fermentation conditions and post-treatment methods on porosity of bacterial cellulose membrane

Design and Printing Strategies in 3D Bioprinting of Cell‐Hydrogels: A Review

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